Abstract：
:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile study using GeneChip Wheat Genome Array and Fluidigm 96.96 Dynamic Arrays was performed to obtain insights into the mode of action of Pm3b and to elucidate the molecular basis of pleiotropic effects observed in three out of four independent transgenic events under field conditions. A cluster analysis of the microarray data and a principal component analysis of the Fluidigm 96.96 Dynamic Arrays data showed that transgenic lines and null segregants grouped together. The microarray analysis of samples from fungicide-treated plants revealed that significantly fewer genes were differentially expressed in Pm3b#1 than in Pm3b#2, which had a pleiotropic phenotype in the field, compared to their null segregants. Together, our data provide evidence that the environment influenced gene expression in the Pm3b lines more than the transgene itself.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Quijano CD,Brunner S,Keller B,Gruissem W,Sautter Cdoi
10.1007/s11248-014-9821-0subject
Has Abstractpub_date
2015-02-01 00:00:00pages
87-97issue
1eissn
0962-8819issn
1573-9368journal_volume
24pub_type
杂志文章abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9563-1
更新日期：2012-06-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9494-x
更新日期：2011-12-01 00:00:00
abstract：:We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9275-y
更新日期：2009-12-01 00:00:00
abstract：:Standard methods to mark Xenopus laevis individuals like tattooing or clipping toenails are inappropriate for the fast growing and regenerating small froglets and the previously used transponders are too large. In this study we successfully adapted micro transponders to tag these animals. Using these new transponders ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9061-z
更新日期：2007-08-01 00:00:00
abstract：:The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2), is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01980212
更新日期：1996-11-01 00:00:00
abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00198-8
更新日期：2020-06-01 00:00:00
abstract：:Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9091-1
更新日期：2008-04-01 00:00:00
abstract：:Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9556-0
更新日期：2012-06-01 00:00:00
abstract：:Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-0021-4
更新日期：2006-08-01 00:00:00
abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008915007271
更新日期：1999-06-01 00:00:00
abstract：:The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-015-9899-z
更新日期：2015-10-01 00:00:00
abstract：:One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9932-x
更新日期：2016-06-01 00:00:00
abstract：:We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9517-7
更新日期：2012-02-01 00:00:00
abstract：:To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title：Transgenic research
pub_type： 信件
doi：10.1007/s11248-012-9605-3
更新日期：2012-12-01 00:00:00
abstract：:Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022904805028
更新日期：2003-04-01 00:00:00
abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9942-8
更新日期：2016-08-01 00:00:00
abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9388-3
更新日期：2011-02-01 00:00:00
abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976498
更新日期：1995-01-01 00:00:00
abstract：:Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976173
更新日期：1993-11-01 00:00:00
abstract：:The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/b:trag.0000040053.23687.9c
更新日期：2004-08-01 00:00:00
abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-006-0024-1
更新日期：2006-08-01 00:00:00
abstract：:To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9328-2
更新日期：2010-06-01 00:00:00
abstract：:The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9909-1
更新日期：2015-12-01 00:00:00
abstract：:Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9763-y
更新日期：2014-02-01 00:00:00
abstract：:Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vasc...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9224-1
更新日期：2009-04-01 00:00:00
abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9117-8
更新日期：2008-06-01 00:00:00
abstract：:Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9403-8
更新日期：2011-06-01 00:00:00
abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1025814814823
更新日期：2003-10-01 00:00:00
abstract：:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-5413-8
更新日期：2005-04-01 00:00:00
abstract：:The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9461-y
更新日期：2011-08-01 00:00:00
