Aberrant transcription and post-transcriptional processing of hepatitis C virus non-structural genes in transgenic mice.


:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been achieved in transgenic approaches for development of rodent models of HCV, transgenic expression of the complete HCV polyprotein or an entire set of the viral non-structural (NS) proteins continues to be a serious challenge. Using northern blot and 5' rapid amplification of cDNA ends (RACE), we unraveled two possible mechanisms that can impede HCV NS transgene expression in the mouse liver. Several truncated transcripts are produced from alternate transcription start sites along the HCV NS sequence within the murine environment, in vivo. Translation of these shorter transcripts is blocked either by the positioning of a contextual stop codon or through a shift in the reading frame. In addition, the complete NS transcript undergoes trans-splicing through 5' recombination with a non-transgene-derived, spliced leader sequence that appends a potential stop codon upstream of the translation start. These findings thus demonstrate that HCV NS-derived transgenes are subject to aberrant transcriptional initiation and post-transcriptional processing in the nucleus of a mouse host. Strategies to prevent such aberrant transcription start/RNA processing might be key to the development of a successful HCV transgenic mouse model.


Transgenic Res


Transgenic research


Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS




Has Abstract


2011-12-01 00:00:00












  • Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.

    abstract::The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

    更新日期:2011-12-01 00:00:00

  • The advances and perspectives of recombinant protein production in the silk gland of silkworm Bombyx mori.

    abstract::The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Xu H

    更新日期:2014-10-01 00:00:00

  • The effect of additional virulence genes on transformation efficiency, transgene integration and expression in rice plants using the pGreen/pSoup dual binary vector system.

    abstract::We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Vain P,Harvey A,Worland B,Ross S,Snape JW,Lonsdale D

    更新日期:2004-12-01 00:00:00

  • Development of transgenic watermelon resistant to Cucumber mosaic virus and Watermelon mosaic virus by using a single chimeric transgene construct.

    abstract::Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lin CY,Ku HM,Chiang YH,Ho HY,Yu TA,Jan FJ

    更新日期:2012-10-01 00:00:00

  • A germline-competent embryonic stem cell line from NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ (NSG) mice.

    abstract::The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Landel CP,Dunlap J,Patton JB,Manser T

    更新日期:2013-02-01 00:00:00

  • Genetically engineered livestock for biomedical models.

    abstract::To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Rogers CS

    更新日期:2016-06-01 00:00:00

  • Bovine alpha s1-casein gene sequences direct high level expression of human granulocyte-macrophage colony-stimulating factor in the milk of transgenic mice.

    abstract::The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

    更新日期:1997-01-01 00:00:00

  • Genetic polymorphisms among C57BL/6 mouse inbred strains.

    abstract::Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zurita E,Chagoyen M,Cantero M,Alonso R,González-Neira A,López-Jiménez A,López-Moreno JA,Landel CP,Benítez J,Pazos F,Montoliu L

    更新日期:2011-06-01 00:00:00

  • Inductively coupled mass spectrometry analysis of biometals in conditional Hamp1 and Hamp1 and Hamp2 transgenic mouse models.

    abstract::Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lu S,Seravalli J,Harrison-Findik D

    更新日期:2015-08-01 00:00:00

  • Specific pathogen free conditions prevent transthyretin amyloidosis in mouse models.

    abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Inoue S,Ohta M,Li Z,Zhao G,Takaoka Y,Sakashita N,Miyakawa K,Takada K,Tei H,Suzuki M,Masuoka M,Sakaki Y,Takahashi K,Yamamura K

    更新日期:2008-10-01 00:00:00

  • Evaluation of the potential for interspecific hybridization between Camelina sativa and related wild Brassicaceae in anticipation of field trials of GM camelina.

    abstract::Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Julié-Galau S,Bellec Y,Faure JD,Tepfer M

    更新日期:2014-02-01 00:00:00

  • Expression of the isopentenyl transferase gene is regulated by auxin in transgenic tobacco tissues.

    abstract::The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zhang XD,Letham DS,Zhang R,Higgins TJ

    更新日期:1996-01-01 00:00:00

  • Effect of DNA concentration on transgenesis rates in mice and pigs.

    abstract::A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Nottle MB,Haskard KA,Verma PJ,Du ZT,Grupen CG,McIlfatrick SM,Ashman RJ,Harrison SJ,Barlow H,Wigley PL,Lyons IG,Cowan PJ,Crawford RJ,Tolstoshev PL,Pearse MJ,Robins AJ,d'Apice AJ

    更新日期:2001-12-01 00:00:00

  • Introduction and expression of the bacterial glyoxylate cycle genes in transgenic mice.

    abstract::The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC and malate synthase (MS; EC, is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Saini KS,Byrne CR,Leish Z,Pruss CA,Rigby NW,Brownlee AG,Nancarrow CD,Ward KA

    更新日期:1996-11-01 00:00:00

  • BAC mediated transgenic Large White boars with FSHα/β genes from Chinese Erhualian pigs.

    abstract::Follicle-stimulating hormone (FSH) is a critical hormone regulating reproduction in mammals. Transgenic mice show that overexpression of FSH can improve female fecundity. Using a bacterial artificial chromosome (BAC) system and somatic cell nuclear transfer, we herein generated 67 Large White transgenic (TG) boars har...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Xu P,Li Q,Jiang K,Yang Q,Bi M,Jiang C,Wang X,Wang C,Li L,Qiao C,Gong H,Xing Y,Ren J

    更新日期:2016-10-01 00:00:00

  • Gene targeting, genome editing: from Dolly to editors.

    abstract::One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Tan W,Proudfoot C,Lillico SG,Whitelaw CB

    更新日期:2016-06-01 00:00:00

  • Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells.

    abstract::We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Dorin JR,Dickinson P,Emslie E,Clarke AR,Dobbie L,Hooper ML,Halford S,Wainwright BJ,Porteous DJ

    更新日期:1992-03-01 00:00:00

  • Synonymous mutation gene design to overexpress ACCase in creeping bentgrass to obtain resistance to ACCase-inhibiting herbicides.

    abstract::Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Heckart DL,Schwartz BM,Raymer PL,Parrott WA

    更新日期:2016-08-01 00:00:00

  • Muscle-specific transgenic expression of porcine myostatin propeptide enhances muscle growth in mice.

    abstract::Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Wang K,Li Z,Li Y,Zeng J,He C,Yang J,Liu D,Wu Z

    更新日期:2013-10-01 00:00:00

  • Transgenic production of arachidonic acid in oilseeds.

    abstract::We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Petrie JR,Shrestha P,Belide S,Mansour MP,Liu Q,Horne J,Nichols PD,Singh SP

    更新日期:2012-02-01 00:00:00

  • A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature.

    abstract::The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

    更新日期:2008-06-01 00:00:00

  • Cell-type specific activation of the polyomavirus F9-1 regulatory region in transgenic mice.

    abstract::To characterize the activity of the polyomavirus regulatory region, two hybrid marker genes were constructed. In the first construct, the early promoter regulates expression of the CAT gene and the late promoter regulates expression of the lacZ gene. In the second construct, the lacZ gene was placed under the control ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Neubauer A,Manitz MP,Napirei M,Krippl B

    更新日期:1996-11-01 00:00:00

  • Protection against vascular leak in neprilysin transgenic mice with complex overexpression pattern.

    abstract::Neprilysin (NEP) is a cell surface metallopeptidase found in many tissues. Based mostly on pharmacological manipulations, NEP has been thought to protect blood vessels from plasma extravasation. We have suggested that NEP may protect against pulmonary vascular injury. However, these prior studies did not utilize mice ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Wick MJ,Loomis ZL,Harral JW,Le M,Wehling CA,Miller YE,Dempsey EC

    更新日期:2016-12-01 00:00:00

  • Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (Oreochromis niloticus).

    abstract::Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be relate...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Rahman MA,Hwang GL,Razak SA,Sohm F,Maclean N

    更新日期:2000-12-01 00:00:00

  • The expression of a mammalian proteinase inhibitor, bovine spleen trypsin inhibitor in tobacco and its effects on Helicoverpa armigera larvae.

    abstract::The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Christeller JT,Burgess EP,Mett V,Gatehouse HS,Markwick NP,Murray C,Malone LA,Wright MA,Philip BA,Watt D,Gatehouse LN,Lövei GL,Shannon AL,Phung MM,Watson LM,Laing WA

    更新日期:2002-04-01 00:00:00

  • Transgenic rice expressing the cry2AX1 gene confers resistance to multiple lepidopteran pests.

    abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Chakraborty M,Reddy PS,Mustafa G,Rajesh G,Narasu VM,Udayasuriyan V,Rana D

    更新日期:2016-10-01 00:00:00

  • Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola.

    abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

    更新日期:2004-06-01 00:00:00

  • Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori.

    abstract::To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

    更新日期:2010-06-01 00:00:00

  • The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco.

    abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Carstens M,Vivier MA,Pretorius IS

    更新日期:2003-08-01 00:00:00

  • A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.

    abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

    journal_title:Transgenic research

    pub_type: 杂志文章,meta分析


    authors: Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

    更新日期:2014-04-01 00:00:00