Aberrant transcription and post-transcriptional processing of hepatitis C virus non-structural genes in transgenic mice.


:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been achieved in transgenic approaches for development of rodent models of HCV, transgenic expression of the complete HCV polyprotein or an entire set of the viral non-structural (NS) proteins continues to be a serious challenge. Using northern blot and 5' rapid amplification of cDNA ends (RACE), we unraveled two possible mechanisms that can impede HCV NS transgene expression in the mouse liver. Several truncated transcripts are produced from alternate transcription start sites along the HCV NS sequence within the murine environment, in vivo. Translation of these shorter transcripts is blocked either by the positioning of a contextual stop codon or through a shift in the reading frame. In addition, the complete NS transcript undergoes trans-splicing through 5' recombination with a non-transgene-derived, spliced leader sequence that appends a potential stop codon upstream of the translation start. These findings thus demonstrate that HCV NS-derived transgenes are subject to aberrant transcriptional initiation and post-transcriptional processing in the nucleus of a mouse host. Strategies to prevent such aberrant transcription start/RNA processing might be key to the development of a successful HCV transgenic mouse model.


Transgenic Res


Transgenic research


Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS




Has Abstract


2011-12-01 00:00:00












  • Establishment and characterization of fetal fibroblast cell lines for generating human lysozyme transgenic goats by somatic cell nuclear transfer.

    abstract::This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Liu J,Luo Y,Zheng L,Liu Q,Yang Z,Wang Y,Su J,Quan F,Zhang Y

    更新日期:2013-10-01 00:00:00

  • Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse.

    abstract::Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

    更新日期:2010-08-01 00:00:00

  • Protection against vascular leak in neprilysin transgenic mice with complex overexpression pattern.

    abstract::Neprilysin (NEP) is a cell surface metallopeptidase found in many tissues. Based mostly on pharmacological manipulations, NEP has been thought to protect blood vessels from plasma extravasation. We have suggested that NEP may protect against pulmonary vascular injury. However, these prior studies did not utilize mice ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Wick MJ,Loomis ZL,Harral JW,Le M,Wehling CA,Miller YE,Dempsey EC

    更新日期:2016-12-01 00:00:00

  • Production of two vaccinating recombinant rotavirus proteins in the milk of transgenic rabbits.

    abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Soler E,Le Saux A,Guinut F,Passet B,Cohen R,Merle C,Charpilienne A,Fourgeux C,Sorel V,Piriou A,Schwartz-Cornil I,Cohen J,Houdebine LM

    更新日期:2005-12-01 00:00:00

  • Improved paper pulp from plants with suppressed cinnamoyl-CoA reductase or cinnamyl alcohol dehydrogenase.

    abstract::Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

    更新日期:2002-10-01 00:00:00

  • Phytohormones and rice crop yield: strategies and opportunities for genetic improvement.

    abstract::To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Sakamoto T

    更新日期:2006-08-01 00:00:00

  • A plant transformation vector with a minimal T-DNA.

    abstract::Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Düring K

    更新日期:1994-03-01 00:00:00

  • Constitutive expression of antimicrobial peptide PR-39 in transgenic mice significantly enhances resistance to bacterial infection and promotes growth.

    abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

    更新日期:2018-10-01 00:00:00

  • Plant heat shock protein 70 as carrier for immunization against a plant-expressed reporter antigen.

    abstract::Mammalian Heat Shock Proteins (HSP), have potent immune-stimulatory properties due to the natural capability to associate with polypeptides and bind receptors on antigen presenting cells. The present study was aimed to explore whether plant HSP, and in particular HSP70, share similar properties. We wanted in particula...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Buriani G,Mancini C,Benvenuto E,Baschieri S

    更新日期:2011-04-01 00:00:00

  • Characterization of stable fluorescent transgenic marine medaka (Oryzias dancena) lines carrying red fluorescent protein gene driven by myosin light chain 2 promoter.

    abstract::Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Cho YS,Lee SY,Kim DS,Nam YK

    更新日期:2013-08-01 00:00:00

  • Pig gene knockout by rAAV-mediated homologous recombination: comparison of BRCA1 gene knockout efficiency in Yucatan and Göttingen fibroblasts with slightly different target sequences.

    abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Luo Y,Bolund L,Sørensen CB

    更新日期:2012-06-01 00:00:00

  • Analysis of the maize polyubiquitin-1 promoter heat shock elements and generation of promoter variants with modified expression characteristics.

    abstract::The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Streatfield SJ,Magallanes-Lundback ME,Beifuss KK,Brooks CA,Harkey RL,Love RT,Bray J,Howard JA,Jilka JM,Hood EE

    更新日期:2004-08-01 00:00:00

  • Ballistic transfection of avian primordial germ cell in ovo.

    abstract::In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Li Y,Behnam J,Simkiss K

    更新日期:1995-01-01 00:00:00

  • Knock-in of diphteria toxin A chain gene at Ins2 locus: effects on islet development and localization of Ins2 expression in the brain.

    abstract::We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lamotte L,Jackerott M,Bucchini D,Jami J,Joshi RL,Deltour L

    更新日期:2004-10-01 00:00:00

  • Overexpression of antimicrobial lytic peptides protects grapevine from Pierce's disease under greenhouse but not field conditions.

    abstract::Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Li ZT,Hopkins DL,Gray DJ

    更新日期:2015-10-01 00:00:00

  • Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola.

    abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

    更新日期:2004-06-01 00:00:00

  • In vivo characterization of plant promoter element interaction using synthetic promoters.

    abstract::Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Cazzonelli CI,Velten J

    更新日期:2008-06-01 00:00:00

  • Molecular characterization of transgenic shallots (Allium cepa L.) by adaptor ligation PCR (AL-PCR) and sequencing of genomic DNA flanking T-DNA borders.

    abstract::Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zheng SJ,Henken B,Sofiari E,Jacobsen E,Krens FA,Kik C

    更新日期:2001-06-01 00:00:00

  • Systemic overexpression of antizyme 1 in mouse reduces ornithine decarboxylase activity without major changes in tissue polyamine homeostasis.

    abstract::Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pietilä M,Dhungana H,Uimari A,Sironen R,Alhonen L

    更新日期:2014-02-01 00:00:00

  • Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice.

    abstract::Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pan PW,Käyrä K,Leinonen J,Nissinen M,Parkkila S,Rajaniemi H

    更新日期:2011-06-01 00:00:00

  • The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco.

    abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Carstens M,Vivier MA,Pretorius IS

    更新日期:2003-08-01 00:00:00

  • Tissue specific high level expression of a full length human endogenous retrovirus genome transgene, HERV-R, under control of its own promoter in rats.

    abstract::Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

    更新日期:2003-06-01 00:00:00

  • Transgenic mice over-expressing endothelin-1 in testis transactivated by a Cre/loxP system showed decreased testicular capillary blood flow.

    abstract::It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

    更新日期:2004-04-01 00:00:00

  • Bovine alpha s1-casein gene sequences direct high level expression of human granulocyte-macrophage colony-stimulating factor in the milk of transgenic mice.

    abstract::The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

    更新日期:1997-01-01 00:00:00

  • Tritrophic interactions between transgenic potato expressing snowdrop lectin (GNA), an aphid pest (peach-potato aphid; Myzus persicae (Sulz.) and a beneficial predator (2-spot ladybird; Adalia bipunctata L.).

    abstract::Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Down RE,Ford L,Woodhouse SD,Davison GM,Majerus ME,Gatehouse JA,Gatehouse AM

    更新日期:2003-04-01 00:00:00

  • Development of transgenic sweet potato with multiple virus resistance in South Africa (SA).

    abstract::Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Sivparsad BJ,Gubba A

    更新日期:2014-04-01 00:00:00

  • Accumulation of vitamin E in potato (Solanum tuberosum) tubers.

    abstract::Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Crowell EF,McGrath JM,Douches DS

    更新日期:2008-04-01 00:00:00

  • Environmental impact of herbicide regimes used with genetically modified herbicide-resistant maize.

    abstract::With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Devos Y,Cougnon M,Vergucht S,Bulcke R,Haesaert G,Steurbaut W,Reheul D

    更新日期:2008-12-01 00:00:00

  • The effect of additional virulence genes on transformation efficiency, transgene integration and expression in rice plants using the pGreen/pSoup dual binary vector system.

    abstract::We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Vain P,Harvey A,Worland B,Ross S,Snape JW,Lonsdale D

    更新日期:2004-12-01 00:00:00

  • Identifying and genotyping transgene integration loci.

    abstract::The random germline integration of genetically engineered transgenes has been a powerful technique to study the role of particular genes in variety of biological processes. Although the identification of the transgene insertion site is often not essential for functional analysis of the transgene, identifying the site ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Liang Z,Breman AM,Grimes BR,Rosen ED

    更新日期:2008-10-01 00:00:00