Abstract:
:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understanding of the molecular basis for breadmaking quality in wheat and feed quality in barley, and also provided genes encoding key proteins that determine quality. The control of expression of these genes has been studied in transgenic tobacco plants and by transient expression in cereal protoplasts, providing the basis for the production of transgenic cereals with improved quality characteristics.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis Mdoi
10.1007/BF01976021subject
Has Abstractpub_date
1994-01-01 00:00:00pages
3-12issue
1eissn
0962-8819issn
1573-9368journal_volume
3pub_type
杂志文章,评审abstract::Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-8293-7
更新日期:2005-10-01 00:00:00
abstract::We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-1773-y
更新日期:2005-12-01 00:00:00
abstract::Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000005146.05655.7d
更新日期:2003-12-01 00:00:00
abstract::Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9441-2
更新日期:2011-06-01 00:00:00
abstract::The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01979922
更新日期:1996-01-01 00:00:00
abstract::A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976765
更新日期:1994-11-01 00:00:00
abstract::The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000040053.23687.9c
更新日期:2004-08-01 00:00:00
abstract::Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9364-y
更新日期:2010-10-01 00:00:00
abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9556-0
更新日期:2012-06-01 00:00:00
abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1024220023057
更新日期:2003-08-01 00:00:00
abstract::Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thali...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9085-z
更新日期:2008-02-01 00:00:00
abstract::At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-3913-9
更新日期:2006-04-01 00:00:00
abstract::The beta-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicologic...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1008832711805
更新日期:1998-05-01 00:00:00
abstract::Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9800-5
更新日期:2014-08-01 00:00:00
abstract::In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0039-9
更新日期:2017-12-01 00:00:00
abstract::This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), t...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008838616203
更新日期:1998-07-01 00:00:00
abstract::A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1013007329936
更新日期:2001-12-01 00:00:00
abstract::Standard methods to mark Xenopus laevis individuals like tattooing or clipping toenails are inappropriate for the fast growing and regenerating small froglets and the previously used transponders are too large. In this study we successfully adapted micro transponders to tag these animals. Using these new transponders ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9061-z
更新日期:2007-08-01 00:00:00
abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4350-5
更新日期:2005-10-01 00:00:00
abstract::Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9675-2
更新日期:2013-08-01 00:00:00
abstract::The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5788-6
更新日期:2005-02-01 00:00:00
abstract::Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008951732020
更新日期:2001-04-01 00:00:00
abstract::The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018446325492
更新日期:1997-05-01 00:00:00
abstract::The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9477-3
更新日期:2011-10-01 00:00:00
abstract::Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9943-7
更新日期:2016-08-01 00:00:00
abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9954-4
更新日期:2016-10-01 00:00:00
abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9180-9
更新日期:2008-10-01 00:00:00
abstract::Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9117-8
更新日期:2008-06-01 00:00:00
abstract::To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02528779
更新日期:1992-05-01 00:00:00
abstract::Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00141-6
更新日期:2019-08-01 00:00:00