Abstract:
:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce homozygous lines. The population with the MAR construct had a significantly higher percentage of TSWV resistant plants in the R1 generation than the nonMAR population. Each resistant line was advanced to the R4 generation, and significantly fewer MAR lines lost resistance over generations compared to the nonMAR population. Lines with TSWV resistance in growth chamber tests were also resistant in field trials. Two lines that were resistant in the R1 generation and susceptible in the R4 were examined in more detail in order to determine if transcriptional silencing of the transgene was occurring in the later generation. Short interfering 21-25 nt RNAs from the transgene that are characteristic of post-transcriptional gene silencing (PTGS) were present in the resistant R1 plants, but not the susceptible R4 plants, indicating that virus resistance was associated with PTGS of the transgene. Loss of resistance was accompanied by an increase in promoter methylation in both lines. In line M41, the transgene was fully silenced at the transcriptional level in the R4 as shown by nuclear run-on assays. In line NM13, transgene transcription and RNA accumulation was still present in the R4 generation, but the level of transcription was not sufficient to trigger PTGS, suggesting that this line may have partial transcriptional silencing. These results are consistent with the concept that MARs may prevent transcriptional silencing.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Levin JS,Thompson WF,Csinos AS,Stephenson MG,Weissinger AKdoi
10.1007/s11248-004-5413-8subject
Has Abstractpub_date
2005-04-01 00:00:00pages
193-206issue
2eissn
0962-8819issn
1573-9368journal_volume
14pub_type
杂志文章abstract::Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01974093
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-0389-6
更新日期:2005-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0087-9
更新日期:2018-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章,评审
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journal_title:Transgenic research
pub_type: 信件,评审
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更新日期:2010-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9686-z
更新日期:2013-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008824509988
更新日期:1998-03-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008972010351
更新日期:2000-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11248-015-9905-5
更新日期:2015-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976173
更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1015210919077
更新日期:2002-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018461201385
更新日期:1997-01-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9091-1
更新日期:2008-04-01 00:00:00
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journal_title:Transgenic research
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更新日期:2008-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008998832552
更新日期:2001-01-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9685-0
更新日期:2013-08-01 00:00:00