Abstract:
:The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be widely used in the biological, biotechnical and pharmaceutical application fields. It's a very active area to express recombinant proteins using the silk gland as a bioreactor, and great progress has been achieved recently. This review recapitulates the progress of producing recombinant proteins and silk-based biomaterials in the silk gland of silkworm in addition to the construction of expression systems. Current challenges and future trends in the production of valuable recombinant proteins using transgenic silkworms are also discussed.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Xu Hdoi
10.1007/s11248-014-9826-8subject
Has Abstractpub_date
2014-10-01 00:00:00pages
697-706issue
5eissn
0962-8819issn
1573-9368journal_volume
23pub_type
杂志文章,评审abstract::Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1023381819572
更新日期:2003-06-01 00:00:00
abstract::The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018423015014
更新日期:1997-09-01 00:00:00
abstract::Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4349-y
更新日期:2005-08-01 00:00:00
abstract::There has been considerable interest in pursuing phospholamban as a putative therapeutic target for overcoming depressed calcium handling in human heart failure. Studies predominantly done in mice have shown that phospholamban is a key regulator of sarcoplasmic reticulum calcium cycling and cardiac function. However, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9139-2
更新日期:2008-04-01 00:00:00
abstract::Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008951732020
更新日期:2001-04-01 00:00:00
abstract::Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9942-8
更新日期:2016-08-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9494-x
更新日期:2011-12-01 00:00:00
abstract::The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008915007271
更新日期:1999-06-01 00:00:00
abstract::The ability to tightly control transgene expression in vivo provides an opportunity to determine the role of certain gene products at different times during development and/or in response to different stimuli. We have characterized and evaluated a tetracycline-responsive endothelial-specific binary system during mouse...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008837410485
更新日期:1998-11-01 00:00:00
abstract::Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9709-4
更新日期:2013-10-01 00:00:00
abstract::Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00198-8
更新日期:2020-06-01 00:00:00
abstract::The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2), is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01980212
更新日期:1996-11-01 00:00:00
abstract::Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/BF01976021
更新日期:1994-01-01 00:00:00
abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9275-y
更新日期:2009-12-01 00:00:00
abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9556-0
更新日期:2012-06-01 00:00:00
abstract::Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9441-2
更新日期:2011-06-01 00:00:00
abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1024220023057
更新日期:2003-08-01 00:00:00
abstract::Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To furth...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9054-y
更新日期:2007-10-01 00:00:00
abstract::The random germline integration of genetically engineered transgenes has been a powerful technique to study the role of particular genes in variety of biological processes. Although the identification of the transgene insertion site is often not essential for functional analysis of the transgene, identifying the site ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9190-7
更新日期:2008-10-01 00:00:00
abstract::To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-016-9928-6
更新日期:2016-06-01 00:00:00
abstract::Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016633410041
更新日期:2001-06-01 00:00:00
abstract::The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9909-1
更新日期:2015-12-01 00:00:00
abstract::T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1025814814823
更新日期:2003-10-01 00:00:00
abstract::As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-007-9122-y
更新日期:2007-12-01 00:00:00
abstract::Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9879-3
更新日期:2015-08-01 00:00:00
abstract::Follicle-stimulating hormone (FSH) is a critical hormone regulating reproduction in mammals. Transgenic mice show that overexpression of FSH can improve female fecundity. Using a bacterial artificial chromosome (BAC) system and somatic cell nuclear transfer, we herein generated 67 Large White transgenic (TG) boars har...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9963-3
更新日期:2016-10-01 00:00:00
abstract::A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01969712
更新日期:1996-05-01 00:00:00
abstract::Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9364-y
更新日期:2010-10-01 00:00:00
abstract::The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02512996
更新日期:1991-12-01 00:00:00