Abstract:
:To characterize the activity of the polyomavirus regulatory region, two hybrid marker genes were constructed. In the first construct, the early promoter regulates expression of the CAT gene and the late promoter regulates expression of the lacZ gene. In the second construct, the lacZ gene was placed under the control of the early promoter. The fusion constructs were introduced into the mouse germline. Gene expression was analysed in the generated transgenic mice. A pronounced cell-type specific activation of the transcriptional control region was found in different tissues of the developing embryo and in the adult animal. The control region is recognized and activated in early preimplantation embryos. Around the time of implantation, sequential activation of the Py regulatory region was first observed in differentiating cells. Stage- and tissue-specific expression were noted later in embryonic development. Comparing reporter gene expression on the single-cell level, the different viral promoters display identical expression patterns throughout ontogenesis. Quantitative analysis revealed that marker gene expression from the late promoter was significantly higher than from the early promoter. Furthermore, the cell-type specificity of the control region is not altered in the presence of its regulatory protein, the LT.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Neubauer A,Manitz MP,Napirei M,Krippl Bdoi
10.1007/BF01980202subject
Has Abstractpub_date
1996-11-01 00:00:00pages
373-83issue
6eissn
0962-8819issn
1573-9368journal_volume
5pub_type
杂志文章abstract::We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-2808-5
更新日期:2004-12-01 00:00:00
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journal_title:Transgenic research
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abstract::Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0007-2
更新日期:2006-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-006-0024-1
更新日期:2006-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000040053.23687.9c
更新日期:2004-08-01 00:00:00
abstract::Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0021-4
更新日期:2006-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9800-5
更新日期:2014-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 信件,评审
doi:10.1007/s11248-009-9297-5
更新日期:2010-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9785-0
更新日期:2014-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11248-007-9107-x
更新日期:2007-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9675-2
更新日期:2013-08-01 00:00:00
abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9014-6
更新日期:2006-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9219-y
更新日期:2009-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008824509988
更新日期:1998-03-01 00:00:00
abstract::Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9117-8
更新日期:2008-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9585-8
更新日期:2012-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1013986627504
更新日期:2002-02-01 00:00:00
abstract::A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976765
更新日期:1994-11-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1025814814823
更新日期:2003-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11248-016-9942-8
更新日期:2016-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9091-1
更新日期:2008-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/BF01976021
更新日期:1994-01-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9954-4
更新日期:2016-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9420-7
更新日期:2011-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9224-1
更新日期:2009-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9828-6
更新日期:2015-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9963-3
更新日期:2016-10-01 00:00:00
abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1024220023057
更新日期:2003-08-01 00:00:00