Abstract:
:We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Ko JH,Lee CS,Kim KH,Pang MG,Koo JS,Fang N,Koo DB,Oh KB,Youn WS,Zheng GD,Park JS,Kim SJ,Han YM,Choi IY,Lim J,Shin ST,Jin SW,Lee KK,Yoo OJdoi
10.1023/a:1008972010351subject
Has Abstractpub_date
2000-06-01 00:00:00pages
215-22issue
3eissn
0962-8819issn
1573-9368journal_volume
9pub_type
杂志文章abstract::Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...
journal_title:Transgenic research
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journal_title:Transgenic research
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