Abstract:
:The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental lines. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Fedorov LM,Haegel-Kronenberger H,Hirchenhain Jdoi
10.1023/a:1018446325492subject
Has Abstractpub_date
1997-05-01 00:00:00pages
223-31issue
3eissn
0962-8819issn
1573-9368journal_volume
6pub_type
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更新日期:2009-02-01 00:00:00
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abstract::Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title:Transgenic research
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pub_type: 杂志文章,评审
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journal_title:Transgenic research
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