Production of two vaccinating recombinant rotavirus proteins in the milk of transgenic rabbits.

abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

journal_title：Transgenic research

authors： Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

更新日期：2008-06-01 00:00:00

abstract：:Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...

journal_title：Transgenic research

authors： Ferry N,Mulligan EA,Stewart CN,Tabashnik BE,Port GR,Gatehouse AM

更新日期：2006-08-01 00:00:00

abstract：:Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...

journal_title：Transgenic research

authors： Patek CE,Saunders PT,Miles CG,Berry RL,Hastie ND,Sharpe RM,Hooper ML

更新日期：2005-10-01 00:00:00

abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

journal_title：Transgenic research

authors： Ahad A,Wolf J,Nick P

更新日期：2003-10-01 00:00:00

abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

journal_title：Transgenic research

authors： Rogers CS

更新日期：2016-06-01 00:00:00

abstract：:The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...

journal_title：Transgenic research

authors： Carstens M,Vivier MA,Pretorius IS

更新日期：2003-08-01 00:00:00

abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

journal_title：Transgenic research

authors： Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

更新日期：2010-08-01 00:00:00

abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...

journal_title：Transgenic research

authors： Cazzonelli CI,Velten J

更新日期：2008-06-01 00:00:00

abstract：:Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...

journal_title：Transgenic research

authors： Thomas AD,Murray JD,Oberbauer AM

更新日期：2005-08-01 00:00:00

abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

journal_title：Transgenic research

authors： Heckart DL,Schwartz BM,Raymer PL,Parrott WA

更新日期：2016-08-01 00:00:00

abstract：:Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...

journal_title：Transgenic research

authors： Hanania U,Velcheva M,Or E,Flaishman M,Sahar N,Perl A

更新日期：2007-08-01 00:00:00

abstract：:We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...

journal_title：Transgenic research

authors： Dorin JR,Dickinson P,Emslie E,Clarke AR,Dobbie L,Hooper ML,Halford S,Wainwright BJ,Porteous DJ

更新日期：1992-03-01 00:00:00

abstract：:Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...

journal_title：Transgenic research

authors： Jiwan D,Roalson EH,Main D,Dhingra A

更新日期：2013-12-01 00:00:00

abstract：:A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...

journal_title：Transgenic research

authors： Nottle MB,Haskard KA,Verma PJ,Du ZT,Grupen CG,McIlfatrick SM,Ashman RJ,Harrison SJ,Barlow H,Wigley PL,Lyons IG,Cowan PJ,Crawford RJ,Tolstoshev PL,Pearse MJ,Robins AJ,d'Apice AJ

更新日期：2001-12-01 00:00:00

abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...

journal_title：Transgenic research

authors： Zhang XD,Letham DS,Zhang R,Higgins TJ

更新日期：1996-01-01 00:00:00

abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

journal_title：Transgenic research

authors： Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

更新日期：2011-02-01 00:00:00

abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

journal_title：Transgenic research

authors： Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

更新日期：1997-01-01 00:00:00

abstract：:Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...

journal_title：Transgenic research

authors： Suttiprapa S,Rinaldi G,Brindley PJ

更新日期：2012-06-01 00:00:00

abstract：:In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...

journal_title：Transgenic research

authors： Cruz BP,Brongar LF,Popiolek P,Gonçalvez BSB,Figueiredo MA,Amaral IPG,Da Rosa VS,Nery LEM,Marins LF

更新日期：2017-12-01 00:00:00

abstract：:Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...

journal_title：Transgenic research

authors： Pietilä M,Dhungana H,Uimari A,Sironen R,Alhonen L

更新日期：2014-02-01 00:00:00

abstract：:Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thali...

journal_title：Transgenic research

authors： Chen LH,Zhang B,Xu ZQ

更新日期：2008-02-01 00:00:00

abstract：:It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

journal_title：Transgenic research

authors： Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

更新日期：2004-04-01 00:00:00

abstract：:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

journal_title：Transgenic research

authors： Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

更新日期：1994-11-01 00:00:00

abstract：:The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...

journal_title：Transgenic research

authors： Streatfield SJ,Magallanes-Lundback ME,Beifuss KK,Brooks CA,Harkey RL,Love RT,Bray J,Howard JA,Jilka JM,Hood EE

更新日期：2004-08-01 00:00:00

abstract：:Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...

journal_title：Transgenic research

authors： Ashikawa I,Mori M,Nakamura S,Abe F

更新日期：2014-08-01 00:00:00

abstract：:Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...

journal_title：Transgenic research

authors： Lin CY,Ku HM,Chiang YH,Ho HY,Yu TA,Jan FJ

更新日期：2012-10-01 00:00:00

abstract：:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

journal_title：Transgenic research

authors： Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

更新日期：2003-06-01 00:00:00

abstract：:Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...

journal_title：Transgenic research

authors： Burket CT,Montgomery JE,Thummel R,Kassen SC,LaFave MC,Langenau DM,Zon LI,Hyde DR

更新日期：2008-04-01 00:00:00

abstract：:This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...

journal_title：Transgenic research

authors： Liu J,Luo Y,Zheng L,Liu Q,Yang Z,Wang Y,Su J,Quan F,Zhang Y

更新日期：2013-10-01 00:00:00

abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

journal_title：Transgenic research

authors： Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

更新日期：2012-08-01 00:00:00