Production of two vaccinating recombinant rotavirus proteins in the milk of transgenic rabbits.


:Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New strategies allowing production of safe recombinant non-replicating rotavirus candidate vaccine are thus clearly needed. In this study we utilized transgenic rabbit milk as a source of rotavirus antigens. Individual transgenic rabbit lines were able to produce several hundreds of micrograms per ml of secreted recombinant VP2 and VP6 proteins in their milk. Viral proteins expressed in our model were immunogenic and were shown to induce a significant reduction in viral antigen shedding after challenge with virulent rotavirus in the adult mouse model. To our knowledge, this is the first report of transgenic mammal bioreactors allowing the rapid co-production of two recombinant viral proteins in milk to be used as a vaccine.


Transgenic Res


Transgenic research


Soler E,Le Saux A,Guinut F,Passet B,Cohen R,Merle C,Charpilienne A,Fourgeux C,Sorel V,Piriou A,Schwartz-Cornil I,Cohen J,Houdebine LM




Has Abstract


2005-12-01 00:00:00












  • Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice.

    abstract::Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pan PW,Käyrä K,Leinonen J,Nissinen M,Parkkila S,Rajaniemi H

    更新日期:2011-06-01 00:00:00

  • Protection against vascular leak in neprilysin transgenic mice with complex overexpression pattern.

    abstract::Neprilysin (NEP) is a cell surface metallopeptidase found in many tissues. Based mostly on pharmacological manipulations, NEP has been thought to protect blood vessels from plasma extravasation. We have suggested that NEP may protect against pulmonary vascular injury. However, these prior studies did not utilize mice ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Wick MJ,Loomis ZL,Harral JW,Le M,Wehling CA,Miller YE,Dempsey EC

    更新日期:2016-12-01 00:00:00

  • Aberrant transcription and post-transcriptional processing of hepatitis C virus non-structural genes in transgenic mice.

    abstract::Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS

    更新日期:2011-12-01 00:00:00

  • A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.

    abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

    journal_title:Transgenic research

    pub_type: 杂志文章,meta分析


    authors: Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

    更新日期:2014-04-01 00:00:00

  • Conditional gene expression in the respiratory epithelium of the mouse.

    abstract::Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Perl AK,Tichelaar JW,Whitsett JA

    更新日期:2002-02-01 00:00:00

  • Canadian regulatory aspects of gene editing technologies.

    abstract::The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ellens KW,Levac D,Pearson C,Savoie A,Strand N,Louter J,Tibelius C

    更新日期:2019-08-01 00:00:00

  • Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis.

    abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pai CW,Chen YH

    更新日期:2010-08-01 00:00:00

  • Partial correction of abnormal cardiac development in caspase-8-deficient mice by cardiomyocyte expression of p 35.

    abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

    更新日期:2005-10-01 00:00:00

  • Molecular-based environmental risk assessment of three varieties of genetically engineered cows.

    abstract::The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

    更新日期:2011-10-01 00:00:00

  • Biosafety of E. coli beta-glucuronidase (GUS) in plants.

    abstract::The beta-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicologic...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Gilissen LJ,Metz PL,Stiekema WJ,Nap JP

    更新日期:1998-05-01 00:00:00

  • Improving seed size, seed weight and seedling emergence in Camelina sativa by overexpressing the Atsob3-6 gene variant.

    abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Sharma Koirala P,Neff MM

    更新日期:2020-08-01 00:00:00

  • Availability of subfertile transgenic rats expressing the c-myc gene as recipients for spermatogonial transplantation.

    abstract::The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hirabayashi M,Yoshizawa Y,Kato M,Tsuchiya T,Nagao S,Hochi S

    更新日期:2009-02-01 00:00:00

  • Fish can be first--advances in fish transgenesis for commercial applications.

    abstract::Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Zbikowska HM

    更新日期:2003-08-01 00:00:00

  • Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field.

    abstract::The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: de Paiva Rolla AA,de Fátima Corrêa Carvalho J,Fuganti-Pagliarini R,Engels C,do Rio A,Marin SR,de Oliveira MC,Beneventi MA,Marcelino-Guimarães FC,Farias JR,Neumaier N,Nakashima K,Yamaguchi-Shinozaki K,Nepomuceno AL

    更新日期:2014-02-01 00:00:00

  • Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.

    abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Suttiprapa S,Rinaldi G,Brindley PJ

    更新日期:2012-06-01 00:00:00

  • Opportunities for manipulating the seed protein composition of wheat and barley in order to improve quality.

    abstract::Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis M

    更新日期:1994-01-01 00:00:00

  • The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco.

    abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Carstens M,Vivier MA,Pretorius IS

    更新日期:2003-08-01 00:00:00

  • Construction of a transgenic pig model overexpressing polycystic kidney disease 2 (PKD2) gene.

    abstract::Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

    更新日期:2013-08-01 00:00:00

  • Molecular characterization of transgenic shallots (Allium cepa L.) by adaptor ligation PCR (AL-PCR) and sequencing of genomic DNA flanking T-DNA borders.

    abstract::Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zheng SJ,Henken B,Sofiari E,Jacobsen E,Krens FA,Kik C

    更新日期:2001-06-01 00:00:00

  • Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits.

    abstract::Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hanania U,Velcheva M,Or E,Flaishman M,Sahar N,Perl A

    更新日期:2007-08-01 00:00:00

  • Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana.

    abstract::Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ling KS,Zhu HY,Gonsalves D

    更新日期:2008-08-01 00:00:00

  • Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola.

    abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

    更新日期:2004-06-01 00:00:00

  • Synonymous mutation gene design to overexpress ACCase in creeping bentgrass to obtain resistance to ACCase-inhibiting herbicides.

    abstract::Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Heckart DL,Schwartz BM,Raymer PL,Parrott WA

    更新日期:2016-08-01 00:00:00

  • Cis effect of lacZ sequences in transgenic mice.

    abstract::Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Paldi A,Deltour L,Jami J

    更新日期:1993-11-01 00:00:00

  • Generation of trangenic Xenopus laevis using the Sleeping Beauty transposon system.

    abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Sinzelle L,Vallin J,Coen L,Chesneau A,Du Pasquier D,Pollet N,Demeneix B,Mazabraud A

    更新日期:2006-12-01 00:00:00

  • Human bcr-abl gene has a lethal effect on embryogenesis.

    abstract::The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

    更新日期:1991-12-01 00:00:00

  • Accumulation of vitamin E in potato (Solanum tuberosum) tubers.

    abstract::Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Crowell EF,McGrath JM,Douches DS

    更新日期:2008-04-01 00:00:00

  • Expression of apoplast-targeted plant defensin MtDef4.2 confers resistance to leaf rust pathogen Puccinia triticina but does not affect mycorrhizal symbiosis in transgenic wheat.

    abstract::Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kaur J,Fellers J,Adholeya A,Velivelli SL,El-Mounadi K,Nersesian N,Clemente T,Shah D

    更新日期:2017-02-01 00:00:00

  • Development of a tightly regulated and highly inducible ecdysone receptor gene switch for plants through the use of retinoid X receptor chimeras.

    abstract::Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To furth...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tavva VS,Dinkins RD,Palli SR,Collins GB

    更新日期:2007-10-01 00:00:00

  • Characterization of stable fluorescent transgenic marine medaka (Oryzias dancena) lines carrying red fluorescent protein gene driven by myosin light chain 2 promoter.

    abstract::Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Cho YS,Lee SY,Kim DS,Nam YK

    更新日期:2013-08-01 00:00:00