Antisense expression of peach mildew resistance locus O (PpMlo1) gene confers cross-species resistance to powdery mildew in Fragaria x ananassa.

abstract：:Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...

journal_title：Transgenic research

authors： Patek CE,Saunders PT,Miles CG,Berry RL,Hastie ND,Sharpe RM,Hooper ML

更新日期：2005-10-01 00:00:00

abstract：:Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vasc...

journal_title：Transgenic research

authors： Wang YA,Zheng JW,Fei ZL,Jiang XQ,Wang ZG,Fei J,Zhang ZY

更新日期：2009-04-01 00:00:00

abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...

journal_title：Transgenic research

authors： Huang W,Olsen BR

更新日期：2015-02-01 00:00:00

abstract：:Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be relate...

journal_title：Transgenic research

authors： Rahman MA,Hwang GL,Razak SA,Sohm F,Maclean N

更新日期：2000-12-01 00:00:00

abstract：:The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...

journal_title：Transgenic research

authors： Fedorov LM,Haegel-Kronenberger H,Hirchenhain J

更新日期：1997-05-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...

journal_title：Transgenic research

authors： Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS

更新日期：2011-12-01 00:00:00

abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...

journal_title：Transgenic research

authors： Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KL

更新日期：1997-09-01 00:00:00

abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...

journal_title：Transgenic research

authors： Sakamoto T

更新日期：2006-08-01 00:00:00

abstract：:Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...

journal_title：Transgenic research

authors： Khan SA,Zafar Y,Briddon RW,Malik KA,Mukhtar Z

更新日期：2006-06-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

journal_title：Transgenic research

authors： He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

更新日期：2013-08-01 00:00:00

abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...

journal_title：Transgenic research

authors： Zhang D,Corlet A,Fouilloux S

更新日期：2008-06-01 00:00:00

abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

journal_title：Transgenic research

authors： O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

更新日期：2002-10-01 00:00:00

abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

journal_title：Transgenic research

authors： Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

更新日期：1998-03-01 00:00:00

abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...

journal_title：Transgenic research

authors： Jan FJ,Pang SZ,Fagoaga C,Gonsalves D

更新日期：1999-06-01 00:00:00

abstract：:There has been considerable interest in pursuing phospholamban as a putative therapeutic target for overcoming depressed calcium handling in human heart failure. Studies predominantly done in mice have shown that phospholamban is a key regulator of sarcoplasmic reticulum calcium cycling and cardiac function. However, ...

journal_title：Transgenic research

authors： Pattison JS,Waggoner JR,James J,Martin L,Gulick J,Osinska H,Klevitsky R,Kranias EG,Robbins J

更新日期：2008-04-01 00:00:00

abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...

journal_title：Transgenic research

authors： Di Nicola-Negri E,Brunetti A,Tavazza M,Ilardi V

更新日期：2005-12-01 00:00:00

abstract：:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

journal_title：Transgenic research

authors： Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

更新日期：2005-10-01 00:00:00

abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

journal_title：Transgenic research

authors： Landel CP,Dunlap J,Patton JB,Manser T

更新日期：2013-02-01 00:00:00

abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...

journal_title：Transgenic research

authors： Goldman D,Hankin M,Li Z,Dai X,Ding J

更新日期：2001-01-01 00:00:00

abstract：:At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...

journal_title：Transgenic research

authors： Dowd PF,Lagrimini LM

更新日期：2006-04-01 00:00:00

abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...

journal_title：Transgenic research

authors： Zhang XD,Letham DS,Zhang R,Higgins TJ

更新日期：1996-01-01 00:00:00

abstract：:Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...

journal_title：Transgenic research

authors： Lin YS,Yang CC,Hsu CC,Hsu JT,Wu SC,Lin CJ,Cheng WT

更新日期：2015-02-01 00:00:00

abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

journal_title：Transgenic research

authors： Zbikowska HM

更新日期：2003-08-01 00:00:00

abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

journal_title：Transgenic research

authors： Luo Y,Bolund L,Sørensen CB

更新日期：2012-06-01 00:00:00

abstract：:Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...

journal_title：Transgenic research

authors： Kenward KD,Brandle J,McPherson J,Davies PL

更新日期：1999-04-01 00:00:00

abstract：:We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...

journal_title：Transgenic research

authors： Vain P,Harvey A,Worland B,Ross S,Snape JW,Lonsdale D

更新日期：2004-12-01 00:00:00

abstract：:The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...

journal_title：Transgenic research

authors： Pai WY,Hsu CC,Lai CY,Chang TZ,Tsai YL,Her GM

更新日期：2013-08-01 00:00:00

abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

journal_title：Transgenic research

authors： Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

更新日期：2012-08-01 00:00:00

abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...

journal_title：Transgenic research

authors： Perl AK,Tichelaar JW,Whitsett JA

更新日期：2002-02-01 00:00:00

abstract：:A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformat...

journal_title：Transgenic research

authors： Skarjinskaia M,Svab Z,Maliga P

更新日期：2003-02-01 00:00:00