Synonymous mutation gene design to overexpress ACCase in creeping bentgrass to obtain resistance to ACCase-inhibiting herbicides.

Abstract:

:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid changes, known as synonymous mutations, may avoid PTGS. For proof of concept, the sequence of acetyl-coA carboxylase (ACCase) from creeping bentgrass (Agrostis stolonifera L.) was altered with synonymous mutations. A native bentgrass ACCase was cloned and used as a template for the modified gene. Wild-type (WT) and modified genes were further modified with a non-synonymous mutation, coding for an isoleucine to leucine substitution at position 1781, known to confer resistance to ACCase-inhibiting herbicides. Five-hundred calli of creeping bentgrass 'Penn A-4' were inoculated with Agrobacterium containing either the WT or modified genes, with or without the herbicide-resistance mutation. Six herbicide-resistant-transgenic events containing the modified gene with the 1781 mutation were obtained. Transcription of the modified ACCase was confirmed in transgenic plants, showing that gene-silencing mechanisms were avoided. Transgenic plants were confirmed to be resistant to the ACCase-inhibiting herbicide, sethoxydim, providing evidence that the modified gene was functional. The result is a novel herbicide-resistance trait and shows that overexpression of a native enzyme with a gene designed with synonymous mutations is possible.

journal_name

Transgenic Res

journal_title

Transgenic research

authors

Heckart DL,Schwartz BM,Raymer PL,Parrott WA

doi

10.1007/s11248-016-9942-8

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

465-76

issue

4

eissn

0962-8819

issn

1573-9368

pii

10.1007/s11248-016-9942-8

journal_volume

25

pub_type

杂志文章
  • Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola.

    abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/b:trag.0000034610.35724.04

    authors: Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

    更新日期:2004-06-01 00:00:00

  • Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes.

    abstract::Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-014-9817-9

    authors: Lin YS,Yang CC,Hsu CC,Hsu JT,Wu SC,Lin CJ,Cheng WT

    更新日期:2015-02-01 00:00:00

  • Improved paper pulp from plants with suppressed cinnamoyl-CoA reductase or cinnamyl alcohol dehydrogenase.

    abstract::Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/a:1020362705497

    authors: O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

    更新日期:2002-10-01 00:00:00

  • Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits.

    abstract::Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-006-9044-0

    authors: Hanania U,Velcheva M,Or E,Flaishman M,Sahar N,Perl A

    更新日期:2007-08-01 00:00:00

  • Identifying and genotyping transgene integration loci.

    abstract::The random germline integration of genetically engineered transgenes has been a powerful technique to study the role of particular genes in variety of biological processes. Although the identification of the transgene insertion site is often not essential for functional analysis of the transgene, identifying the site ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-008-9190-7

    authors: Liang Z,Breman AM,Grimes BR,Rosen ED

    更新日期:2008-10-01 00:00:00

  • Alteration of floral organ identity by over-expression of IbMADS3-1 in tobacco.

    abstract::The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-010-9420-7

    authors: Shin MR,Seo SG,Kim JS,Joen SB,Kang SW,Lee GP,Kwon SY,Kim SH

    更新日期:2011-04-01 00:00:00

  • Antisense expression of peach mildew resistance locus O (PpMlo1) gene confers cross-species resistance to powdery mildew in Fragaria x ananassa.

    abstract::Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-013-9715-6

    authors: Jiwan D,Roalson EH,Main D,Dhingra A

    更新日期:2013-12-01 00:00:00

  • Accumulation of vitamin E in potato (Solanum tuberosum) tubers.

    abstract::Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-007-9091-1

    authors: Crowell EF,McGrath JM,Douches DS

    更新日期:2008-04-01 00:00:00

  • A comparison of the germline potential of differently aged ES cell lines and their transfected descendants.

    abstract::The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/a:1018446325492

    authors: Fedorov LM,Haegel-Kronenberger H,Hirchenhain J

    更新日期:1997-05-01 00:00:00

  • Transgenic pigs expressing β-xylanase in the parotid gland improve nutrient utilization.

    abstract::Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-019-00110-z

    authors: Zhang M,Cai G,Zheng E,Zhang G,Li Y,Li Z,Yang H,Wu Z

    更新日期:2019-04-01 00:00:00

  • A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes.

    abstract::Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-014-9800-5

    authors: Ashikawa I,Mori M,Nakamura S,Abe F

    更新日期:2014-08-01 00:00:00

  • Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors.

    abstract::Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-010-9388-3

    authors: Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

    更新日期:2011-02-01 00:00:00

  • The effect of Bt-transgene introgression on plant growth and reproduction in wild Brassica juncea.

    abstract::This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-014-9858-0

    authors: Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

    更新日期:2015-06-01 00:00:00

  • A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.

    abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

    journal_title:Transgenic research

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11248-013-9757-9

    authors: Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

    更新日期:2014-04-01 00:00:00

  • Regulation of human protein C gene expression by the mouse WAP promoter.

    abstract::A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/BF01976765

    authors: Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

    更新日期:1994-11-01 00:00:00

  • Generation of trangenic Xenopus laevis using the Sleeping Beauty transposon system.

    abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-006-9014-6

    authors: Sinzelle L,Vallin J,Coen L,Chesneau A,Du Pasquier D,Pollet N,Demeneix B,Mazabraud A

    更新日期:2006-12-01 00:00:00

  • Clock genes expression and locomotor activity are altered along the light-dark cycle in transgenic zebrafish overexpressing growth hormone.

    abstract::In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-017-0039-9

    authors: Cruz BP,Brongar LF,Popiolek P,Gonçalvez BSB,Figueiredo MA,Amaral IPG,Da Rosa VS,Nery LEM,Marins LF

    更新日期:2017-12-01 00:00:00

  • Knock-in of diphteria toxin A chain gene at Ins2 locus: effects on islet development and localization of Ins2 expression in the brain.

    abstract::We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-004-9587-x

    authors: Lamotte L,Jackerott M,Bucchini D,Jami J,Joshi RL,Deltour L

    更新日期:2004-10-01 00:00:00

  • Development of transgenic zooplankton Artemia as a bioreactor to produce exogenous protein.

    abstract::Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-010-9479-1

    authors: Chang SH,Lee BC,Chen YD,Lee YC,Tsai HJ

    更新日期:2011-10-01 00:00:00

  • A germline-competent embryonic stem cell line from NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ (NSG) mice.

    abstract::The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-012-9629-8

    authors: Landel CP,Dunlap J,Patton JB,Manser T

    更新日期:2013-02-01 00:00:00

  • Enhanced resistance to Botrytis cinerea mediated by the transgenic expression of the chitinase gene ch5B in strawberry.

    abstract::Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-005-2543-6

    authors: Vellicce GR,Ricci JC,Hernández L,Castagnaro AP

    更新日期:2006-02-01 00:00:00

  • Consequences of gene targeting procedures for behavioural responses and morphological development of newborn mice.

    abstract::In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/a:1012244404020

    authors: van der Meer M,Baumans V,Hofhuis FM,Olivier B,van Zutphen BF

    更新日期:2001-10-01 00:00:00

  • Transgenic mice over-expressing endothelin-1 in testis transactivated by a Cre/loxP system showed decreased testicular capillary blood flow.

    abstract::It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/b:trag.0000026072.01351.10

    authors: Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

    更新日期:2004-04-01 00:00:00

  • Construction of a transgenic pig model overexpressing polycystic kidney disease 2 (PKD2) gene.

    abstract::Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-012-9686-z

    authors: He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

    更新日期:2013-08-01 00:00:00

  • Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse.

    abstract::Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-009-9346-0

    authors: Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

    更新日期:2010-08-01 00:00:00

  • Prey-mediated effects of transgenic canola on a beneficial, non-target, carabid beetle.

    abstract::Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-006-0021-4

    authors: Ferry N,Mulligan EA,Stewart CN,Tabashnik BE,Port GR,Gatehouse AM

    更新日期:2006-08-01 00:00:00

  • Lysozyme transgenic goats' milk positively impacts intestinal cytokine expression and morphology.

    abstract::In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-011-9489-7

    authors: Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD

    更新日期:2011-12-01 00:00:00

  • Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.

    abstract::The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-011-9495-9

    authors: Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

    更新日期:2011-12-01 00:00:00

  • Production of two vaccinating recombinant rotavirus proteins in the milk of transgenic rabbits.

    abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-005-1771-0

    authors: Soler E,Le Saux A,Guinut F,Passet B,Cohen R,Merle C,Charpilienne A,Fourgeux C,Sorel V,Piriou A,Schwartz-Cornil I,Cohen J,Houdebine LM

    更新日期:2005-12-01 00:00:00

  • The advances and perspectives of recombinant protein production in the silk gland of silkworm Bombyx mori.

    abstract::The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审

    doi:10.1007/s11248-014-9826-8

    authors: Xu H

    更新日期:2014-10-01 00:00:00