Abstract:
:Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in morphology to wild type roots but were characterized by a profound abundancy, rapid growth rate and, in some cases, plagiotropic development. Upon challenge inoculation, seedlings showed a considerable delay in symptom development compared to untransformed or vector-transformed seedlings, expressing dsRNA from an unrelated source. The transgenic root system of almost all seedlings contained no or very low virus titer while the non-transformed aerial parts of the same plants were found infected, leading to the conclusion that the hairy roots studied were effectively protected against the virus. This readily applicable novel method forms a plausible approach to preliminarily evaluate transgenic rhizomania resistance before proceeding in transformation and whole plant regeneration of sugar beet, a tedious and time consuming process for such a recalcitrant crop species.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Pavli OI,Panopoulos NJ,Goldbach R,Skaracis GNdoi
10.1007/s11248-010-9364-ysubject
Has Abstractpub_date
2010-10-01 00:00:00pages
915-22issue
5eissn
0962-8819issn
1573-9368journal_volume
19pub_type
杂志文章abstract::A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016671513425
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-2543-6
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9418-1
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journal_title:Transgenic research
pub_type: 杂志文章
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doi:10.1007/s11248-005-3913-9
更新日期:2006-04-01 00:00:00
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-007-9122-y
更新日期:2007-12-01 00:00:00
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journal_title:Transgenic research
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journal_title:Transgenic research
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更新日期:2008-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0051-0
更新日期:2018-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9350-4
更新日期:2010-08-01 00:00:00
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976173
更新日期:1993-11-01 00:00:00
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更新日期:2016-08-01 00:00:00
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doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9629-8
更新日期:2013-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9709-4
更新日期:2013-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4349-y
更新日期:2005-08-01 00:00:00