Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea.

abstract：:Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...

journal_title：Transgenic research

authors： Crowell EF,McGrath JM,Douches DS

更新日期：2008-04-01 00:00:00

abstract：:Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...

journal_title：Transgenic research

authors： Van Eenennaam AL

更新日期：2019-08-01 00:00:00

abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...

journal_title：Transgenic research

authors： Goldman D,Hankin M,Li Z,Dai X,Ding J

更新日期：2001-01-01 00:00:00

abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

journal_title：Transgenic research

authors： O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

更新日期：2002-10-01 00:00:00

abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

journal_title：Transgenic research

authors： Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

更新日期：2010-08-01 00:00:00

abstract：:The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...

journal_title：Transgenic research

authors： de Paiva Rolla AA,de Fátima Corrêa Carvalho J,Fuganti-Pagliarini R,Engels C,do Rio A,Marin SR,de Oliveira MC,Beneventi MA,Marcelino-Guimarães FC,Farias JR,Neumaier N,Nakashima K,Yamaguchi-Shinozaki K,Nepomuceno AL

更新日期：2014-02-01 00:00:00

abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...

journal_title：Transgenic research

authors： Palaudelmàs M,Peñas G,Melé E,Serra J,Salvia J,Pla M,Nadal A,Messeguer J

更新日期：2009-08-01 00:00:00

abstract：:The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...

journal_title：Transgenic research

authors： Shin MR,Seo SG,Kim JS,Joen SB,Kang SW,Lee GP,Kwon SY,Kim SH

更新日期：2011-04-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...

journal_title：Transgenic research

authors： Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS

更新日期：2011-12-01 00:00:00

abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

journal_title：Transgenic research

authors： Sharma Koirala P,Neff MM

更新日期：2020-08-01 00:00:00

abstract：:Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and...

journal_title：Transgenic research

authors： Nanto K,Ebinuma H

更新日期：2008-06-01 00:00:00

abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...

journal_title：Transgenic research

authors： Hisatsune C,Ogawa N,Mikoshiba K

更新日期：2013-12-01 00:00:00

abstract：:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

journal_title：Transgenic research

authors： Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

更新日期：2005-10-01 00:00:00

abstract：:Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...

journal_title：Transgenic research

authors： Melton DW,Ketchen AM,Kind AJ,McEwan C,Paisley D,Selfridge J

更新日期：2001-04-01 00:00:00

abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...

journal_title：Transgenic research

authors： Perl AK,Tichelaar JW,Whitsett JA

更新日期：2002-02-01 00:00:00

abstract：:It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

journal_title：Transgenic research

authors： Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

更新日期：2004-04-01 00:00:00

abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...

journal_title：Transgenic research

authors： Li Y,Behnam J,Simkiss K

更新日期：1995-01-01 00:00:00

abstract：:Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...

journal_title：Transgenic research

authors： Knutson DC,Clagett-Dame M

更新日期：2015-02-01 00:00:00

abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...

journal_title：Transgenic research

authors： Lizen B,Claus M,Jeannotte L,Rijli FM,Gofflot F

更新日期：2015-12-01 00:00:00

abstract：:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

journal_title：Transgenic research

authors： Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

更新日期：2011-12-01 00:00:00

abstract：:Mammalian Heat Shock Proteins (HSP), have potent immune-stimulatory properties due to the natural capability to associate with polypeptides and bind receptors on antigen presenting cells. The present study was aimed to explore whether plant HSP, and in particular HSP70, share similar properties. We wanted in particula...

journal_title：Transgenic research

authors： Buriani G,Mancini C,Benvenuto E,Baschieri S

更新日期：2011-04-01 00:00:00

abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...

journal_title：Transgenic research

authors： Kawazu Y,Fujiyama R,Noguchi Y,Kubota M,Ito H,Fukuoka H

更新日期：2010-04-01 00:00:00

abstract：:A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific...

journal_title：Transgenic research

authors： Chou CY,Horng LS,Tsai HJ

更新日期：2001-08-01 00:00:00

abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

journal_title：Transgenic research

authors： Zbikowska HM

更新日期：2003-08-01 00:00:00

abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

journal_title：Transgenic research

authors： Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

更新日期：1998-03-01 00:00:00

abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

journal_title：Transgenic research

authors： Christensen AH,Quail PH

更新日期：1996-05-01 00:00:00

abstract：:Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...

journal_title：Transgenic research

authors： Lin YS,Yang CC,Hsu CC,Hsu JT,Wu SC,Lin CJ,Cheng WT

更新日期：2015-02-01 00:00:00

abstract：:Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...

journal_title：Transgenic research

authors： Béjar J,Hong Y,Alvarez MC

更新日期：2002-06-01 00:00:00

abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

journal_title：Transgenic research

authors： Rogers CS

更新日期：2016-06-01 00:00:00

abstract：:Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...

journal_title：Transgenic research

authors： Booker HM,Lamb EG,Smyth SJ

更新日期：2017-06-01 00:00:00