Abstract:
:A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific promoter (zebrafish alpha-actin) in transgenic medaka. In addition, treatment with ITR sequence-containing constructs resulted in a dramatic increase in the number of embryos showing uniform GFP-expression at F0. Of the GFP-positive embryos, 34.6% (81/234), 10% (10/60), and 18% (38/212) showed homogenous GFP-expression for the derivative constructs of the cytomegalovirus, alpha-actin, and beta-actin promoters, respectively. As a result of uniform GFP-expression, green fluorescence in founders was (a) extended for an entire lifetime without degradation, and (b) transmitted as a genetic trait to F1 and F2 progeny of some transgenic lines via Mendelian inheritance. A Southern blot analysis revealed a random integration of the transgene into the genome of founders and progeny in both head-to-tail and tail-to-tail concatemerization patterns. Interestingly, some transgenic medaka with uniform and strong fluorescence could be visually noticeable to the unaided eye.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Chou CY,Horng LS,Tsai HJdoi
10.1023/a:1016671513425subject
Has Abstractpub_date
2001-08-01 00:00:00pages
303-15issue
4eissn
0962-8819issn
1573-9368journal_volume
10pub_type
杂志文章abstract::Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0083-0
更新日期:2018-10-01 00:00:00
abstract::We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1022194120518
更新日期:2003-02-01 00:00:00
abstract::In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9489-7
更新日期:2011-12-01 00:00:00
abstract::Phytocystatins are proteinaceous inhibitors of cysteine proteases. They have been implicated in the regulation of plant protein turnover and in defense against pathogens and insects. Here, we have characterized an Arabidopsis phytocystatin family gene, Arabidopsis thaliana phytocystatin 4 (AtCYS4). AtCYS4 was induced ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9735-2
更新日期:2014-02-01 00:00:00
abstract::Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00198-8
更新日期:2020-06-01 00:00:00
abstract::Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9763-y
更新日期:2014-02-01 00:00:00
abstract::Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-7216-y
更新日期:2005-10-01 00:00:00
abstract::Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008951732020
更新日期:2001-04-01 00:00:00
abstract::Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9722-7
更新日期:2014-02-01 00:00:00
abstract::The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018446325492
更新日期:1997-05-01 00:00:00
abstract::During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01973758
更新日期:1995-11-01 00:00:00
abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9563-1
更新日期:2012-06-01 00:00:00
abstract::We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9517-7
更新日期:2012-02-01 00:00:00
abstract::Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5413-8
更新日期:2005-04-01 00:00:00
abstract::Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9978-9
更新日期:2017-02-01 00:00:00
abstract::Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9364-y
更新日期:2010-10-01 00:00:00
abstract::Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000005146.05655.7d
更新日期:2003-12-01 00:00:00
abstract::We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02513027
更新日期:1992-03-01 00:00:00
abstract::We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9275-y
更新日期:2009-12-01 00:00:00
abstract::It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000026072.01351.10
更新日期:2004-04-01 00:00:00
abstract::The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000040053.23687.9c
更新日期:2004-08-01 00:00:00
abstract::The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018461201385
更新日期:1997-01-01 00:00:00
abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9014-6
更新日期:2006-12-01 00:00:00
abstract::The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1024220023057
更新日期:2003-08-01 00:00:00
abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00
abstract::The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01979922
更新日期:1996-01-01 00:00:00
abstract::The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008928121846
更新日期:2000-02-01 00:00:00
abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1008966404981
更新日期:2000-01-01 00:00:00
abstract::A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformat...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1022110402302
更新日期:2003-02-01 00:00:00