Abstract:
:Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that the pta gene had integrated into the plant genome and northern blot analysis revealed transgene expression at various levels in transgenic plants. Genetic analysis confirmed Mendelian segregation of the transgene in T1 progeny. Insect bioassays showed that transgenic plants expressing PTA inhibited significantly the growth of peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic plants expressing pta confer enhanced resistance to aphids. Our study indicates that the pta gene can be used as a supplement to the snowdrop (Galanthus nivalis) lectin gene (gna) in the control of aphids, a sap-sucking insect pest causing significant yield losses of crops.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang Kdoi
10.1023/b:trag.0000005146.05655.7dsubject
Has Abstractpub_date
2003-12-01 00:00:00pages
715-22issue
6eissn
0962-8819issn
1573-9368journal_volume
12pub_type
杂志文章abstract::Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9288-6
更新日期:2010-02-01 00:00:00
abstract::Neprilysin (NEP) is a cell surface metallopeptidase found in many tissues. Based mostly on pharmacological manipulations, NEP has been thought to protect blood vessels from plasma extravasation. We have suggested that NEP may protect against pulmonary vascular injury. However, these prior studies did not utilize mice ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9969-x
更新日期:2016-12-01 00:00:00
abstract::Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1008966404981
更新日期:2000-01-01 00:00:00
abstract::The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9723-6
更新日期:2014-02-01 00:00:00
abstract::Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9106-y
更新日期:2008-06-01 00:00:00
abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00208-9
更新日期:2020-08-01 00:00:00
abstract::Phytocystatins are proteinaceous inhibitors of cysteine proteases. They have been implicated in the regulation of plant protein turnover and in defense against pathogens and insects. Here, we have characterized an Arabidopsis phytocystatin family gene, Arabidopsis thaliana phytocystatin 4 (AtCYS4). AtCYS4 was induced ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9735-2
更新日期:2014-02-01 00:00:00
abstract::Standard methods to mark Xenopus laevis individuals like tattooing or clipping toenails are inappropriate for the fast growing and regenerating small froglets and the previously used transponders are too large. In this study we successfully adapted micro transponders to tag these animals. Using these new transponders ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9061-z
更新日期:2007-08-01 00:00:00
abstract::In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9489-7
更新日期:2011-12-01 00:00:00
abstract::Follicle-stimulating hormone (FSH) is a critical hormone regulating reproduction in mammals. Transgenic mice show that overexpression of FSH can improve female fecundity. Using a bacterial artificial chromosome (BAC) system and somatic cell nuclear transfer, we herein generated 67 Large White transgenic (TG) boars har...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9963-3
更新日期:2016-10-01 00:00:00
abstract::Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9250-7
更新日期:2009-08-01 00:00:00
abstract::While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9101-3
更新日期:2008-04-01 00:00:00
abstract::Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008886629825
更新日期:1999-04-01 00:00:00
abstract::Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-2543-6
更新日期:2006-02-01 00:00:00
abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9556-0
更新日期:2012-06-01 00:00:00
abstract::Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9942-8
更新日期:2016-08-01 00:00:00
abstract::Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9675-2
更新日期:2013-08-01 00:00:00
abstract::This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9858-0
更新日期:2015-06-01 00:00:00
abstract::With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9181-8
更新日期:2008-12-01 00:00:00
abstract::Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9568-9
更新日期:2012-08-01 00:00:00
abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-1771-0
更新日期:2005-12-01 00:00:00
abstract::The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9461-y
更新日期:2011-08-01 00:00:00
abstract::We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-9587-x
更新日期:2004-10-01 00:00:00
abstract::The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9420-7
更新日期:2011-04-01 00:00:00
abstract::We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1022194120518
更新日期:2003-02-01 00:00:00
abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9014-6
更新日期:2006-12-01 00:00:00
abstract::The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5788-6
更新日期:2005-02-01 00:00:00
abstract::The random germline integration of genetically engineered transgenes has been a powerful technique to study the role of particular genes in variety of biological processes. Although the identification of the transgene insertion site is often not essential for functional analysis of the transgene, identifying the site ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9190-7
更新日期:2008-10-01 00:00:00
abstract::Alkaloid concentration of perennial ryegrass herbage is affected by endophyte strain and host plant genotype. However, previous studies suggest that associations between host and endophyte also depends on environmental conditions, especially those affecting nutrient reserves and that water-soluble carbohydrate (WSC) c...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0087-9
更新日期:2018-10-01 00:00:00
abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00