Abstract：
:Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of the plant cell factory, e.g. to produce more of a fine chemical, to produce less of a toxic compound, or even to make new compounds, Engineering of plant secondary metabolism is feasible nowadays, but it requires knowledge of the biosynthetic pathways involved. To increase secondary metabolite production different strategies can be followed, such as overcoming rate limiting steps, reducing flux through competitive pathways, reducing catabolism and overexpression of regulatory genes. For this purpose genes of plant origin can be overexpressed, but also microbial genes have been used successfully. Overexpression of plant genes in microorganisms is another approach, which might be of interest for bioconversion of readily available precursors into valuable fine chemicals. Several examples will be given to illustrate these various approaches. The constraints of metabolic engineering of the plant cell factory will also be discussed. Our limited knowledge of secondary metabolite pathways and the genes involved is one of the main bottlenecks.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Verpoorte R,van der Heijden R,Memelink Jdoi
10.1023/a:1008966404981subject
Has Abstractpub_date
2000-01-01 00:00:00pages
323-43; discussion 321issue
4-5eissn
0962-8819issn
1573-9368journal_volume
9pub_type
杂志文章,评审abstract：:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9182-7
更新日期：2008-12-01 00:00:00
abstract：:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9785-0
更新日期：2014-12-01 00:00:00
abstract：:Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00141-6
更新日期：2019-08-01 00:00:00
abstract：:Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9152-5
更新日期：2008-04-01 00:00:00
abstract：:RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9765-9
更新日期：2014-04-01 00:00:00
abstract：:The introduction of cloned and manipulated genetic material into the germline of an experimental organism is one of the most powerful tools of modern biology. In the case of the fruit fly, Drosophila melanogaster, there is also an unparalleled range of sophisticated genetic tools to facilitate subsequent analysis. In ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/BF01968780
更新日期：1995-05-01 00:00:00
abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9928-6
更新日期：2016-06-01 00:00:00
abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-007-9083-1
更新日期：2007-06-01 00:00:00
abstract：:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-8293-7
更新日期：2005-10-01 00:00:00
abstract：:In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0039-9
更新日期：2017-12-01 00:00:00
abstract：:We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalen...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008972010351
更新日期：2000-06-01 00:00:00
abstract：:The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin protein is efficiently synthesized and precisely regulated is an important aspect that has yet to be fully elu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00175-w
更新日期：2019-12-01 00:00:00
abstract：:Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9978-9
更新日期：2017-02-01 00:00:00
abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9117-8
更新日期：2008-06-01 00:00:00
abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008998832552
更新日期：2001-01-01 00:00:00
abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9441-2
更新日期：2011-06-01 00:00:00
abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018423015014
更新日期：1997-09-01 00:00:00
abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9250-7
更新日期：2009-08-01 00:00:00
abstract：:Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thali...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9085-z
更新日期：2008-02-01 00:00:00
abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9156-1
更新日期：2008-06-01 00:00:00
abstract：:The beta-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicologic...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1008832711805
更新日期：1998-05-01 00:00:00
abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01979922
更新日期：1996-01-01 00:00:00
abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-006-0024-1
更新日期：2006-08-01 00:00:00
abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008915007271
更新日期：1999-06-01 00:00:00
abstract：:This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), t...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008838616203
更新日期：1998-07-01 00:00:00
abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9346-0
更新日期：2010-08-01 00:00:00
abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9489-7
更新日期：2011-12-01 00:00:00
abstract：:Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9879-3
更新日期：2015-08-01 00:00:00
abstract：:Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022904805028
更新日期：2003-04-01 00:00:00
abstract：:The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016374128479
更新日期：2002-08-01 00:00:00
