Lysozyme transgenic goats' milk positively impacts intestinal cytokine expression and morphology.


:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consumption of milk from transgenic goats producing human lysozyme (hLZ) in their milk therefore has the potential to positively impact intestinal health. In order to investigate the effect of hLZ-containing milk on the inflammatory response, young pigs were fed pasteurized milk from hLZ or non-transgenic control goats and quantitative real-time PCR was performed to assess local expression of TNF-α, IL-8, and TGF-β1 in the small intestine. Histological changes were also investigated, specifically looking at villi width, length, crypt depth, and lamina propria thickness along with cell counts for intraepithelial lymphocytes and goblet cells. Significantly higher expression of anti-inflammatory cytokine TGF-β1 was seen in the ileum of pigs fed pasteurized milk containing hLZ (P = 0.0478), along with an increase in intraepithelial lymphocytes (P = 0.0255), and decrease in lamina propria thickness in the duodenum (P = 0.0001). Based on these results we conclude that consuming pasteurized milk containing hLZ does not induce an inflammatory response and improves the health of the small intestine in pigs.


Transgenic Res


Transgenic research


Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD




Has Abstract


2011-12-01 00:00:00












  • Cis effect of lacZ sequences in transgenic mice.

    abstract::Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Paldi A,Deltour L,Jami J

    更新日期:1993-11-01 00:00:00

  • Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors.

    abstract::Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

    更新日期:2011-02-01 00:00:00

  • Is the German suspension of MON810 maize cultivation scientifically justified?

    abstract::We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...

    journal_title:Transgenic research

    pub_type: 信件,评审


    authors: Ricroch A,Bergé JB,Kuntz M

    更新日期:2010-02-01 00:00:00

  • Striatum-specific expression of Cre recombinase using the Gpr88 promoter in mice.

    abstract::We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hisatsune C,Ogawa N,Mikoshiba K

    更新日期:2013-12-01 00:00:00

  • Skeletal defects in Osterix-Cre transgenic mice.

    abstract::Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Huang W,Olsen BR

    更新日期:2015-02-01 00:00:00

  • Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis.

    abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pai CW,Chen YH

    更新日期:2010-08-01 00:00:00

  • High level expression of human apolipoprotein A-I in transgenic rats raises total serum high density lipoprotein cholesterol and lowers rat apolipoprotein A-I.

    abstract::To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Swanson ME,Hughes TE,Denny IS,France DS,Paterniti JR Jr,Tapparelli C,Gfeller P,Bürki K

    更新日期:1992-05-01 00:00:00

  • A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.

    abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

    journal_title:Transgenic research

    pub_type: 杂志文章,meta分析


    authors: Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

    更新日期:2014-04-01 00:00:00

  • Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts.

    abstract::To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...

    journal_title:Transgenic research

    pub_type: 信件


    authors: Fielder TJ,Yi CS,Masumi J,Waymire KG,Chen HW,Wang S,Shi KX,Wallace DC,MacGregor GR

    更新日期:2012-12-01 00:00:00

  • Transgenic rice expressing the cry2AX1 gene confers resistance to multiple lepidopteran pests.

    abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Chakraborty M,Reddy PS,Mustafa G,Rajesh G,Narasu VM,Udayasuriyan V,Rana D

    更新日期:2016-10-01 00:00:00

  • Pig gene knockout by rAAV-mediated homologous recombination: comparison of BRCA1 gene knockout efficiency in Yucatan and Göttingen fibroblasts with slightly different target sequences.

    abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Luo Y,Bolund L,Sørensen CB

    更新日期:2012-06-01 00:00:00

  • Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana.

    abstract::Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ling KS,Zhu HY,Gonsalves D

    更新日期:2008-08-01 00:00:00

  • Perinatal induction of Cre recombination with tamoxifen.

    abstract::Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lizen B,Claus M,Jeannotte L,Rijli FM,Gofflot F

    更新日期:2015-12-01 00:00:00

  • Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid.

    abstract::Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Farré G,Perez-Fons L,Decourcelle M,Breitenbach J,Hem S,Zhu C,Capell T,Christou P,Fraser PD,Sandmann G

    更新日期:2016-08-01 00:00:00

  • Impact of the ahas transgene for herbicides resistance on biological nitrogen fixation and yield of soybean.

    abstract::Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hungria M,Nakatani AS,Souza RA,Sei FB,de Oliveira Chueire LM,Arias CA

    更新日期:2015-02-01 00:00:00

  • Genetically engineered livestock for biomedical models.

    abstract::To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Rogers CS

    更新日期:2016-06-01 00:00:00

  • Generation of trangenic Xenopus laevis using the Sleeping Beauty transposon system.

    abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Sinzelle L,Vallin J,Coen L,Chesneau A,Du Pasquier D,Pollet N,Demeneix B,Mazabraud A

    更新日期:2006-12-01 00:00:00

  • Prey-mediated effects of transgenic canola on a beneficial, non-target, carabid beetle.

    abstract::Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ferry N,Mulligan EA,Stewart CN,Tabashnik BE,Port GR,Gatehouse AM

    更新日期:2006-08-01 00:00:00

  • Agroinfiltration as a tool for transient expression of cre recombinase in vivo.

    abstract::Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kopertekh L,Schiemann J

    更新日期:2005-10-01 00:00:00

  • Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics.

    abstract::Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Ramessar K,Peremarti A,Gómez-Galera S,Naqvi S,Moralejo M,Muñoz P,Capell T,Christou P

    更新日期:2007-06-01 00:00:00

  • Enhanced resistance to Botrytis cinerea mediated by the transgenic expression of the chitinase gene ch5B in strawberry.

    abstract::Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Vellicce GR,Ricci JC,Hernández L,Castagnaro AP

    更新日期:2006-02-01 00:00:00

  • Synonymous mutation gene design to overexpress ACCase in creeping bentgrass to obtain resistance to ACCase-inhibiting herbicides.

    abstract::Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Heckart DL,Schwartz BM,Raymer PL,Parrott WA

    更新日期:2016-08-01 00:00:00

  • Conditional gene expression in the respiratory epithelium of the mouse.

    abstract::Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Perl AK,Tichelaar JW,Whitsett JA

    更新日期:2002-02-01 00:00:00

  • Interleukin 2 promoter/enhancer controlled expression of a synthetic cecropin-class lytic peptide in transgenic mice and subsequent resistance to Brucella abortus.

    abstract::The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KL

    更新日期:1997-09-01 00:00:00

  • A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes.

    abstract::Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ashikawa I,Mori M,Nakamura S,Abe F

    更新日期:2014-08-01 00:00:00

  • Excision of a selective marker in transgenic rice using a novel Cre/loxP system controlled by a floral specific promoter.

    abstract::Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Bai X,Wang Q,Chu C

    更新日期:2008-12-01 00:00:00

  • Type II fish antifreeze protein accumulation in transgenic tobacco does not confer frost resistance.

    abstract::Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kenward KD,Brandle J,McPherson J,Davies PL

    更新日期:1999-04-01 00:00:00

  • Phytohormones and rice crop yield: strategies and opportunities for genetic improvement.

    abstract::To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Sakamoto T

    更新日期:2006-08-01 00:00:00

  • Systemic overexpression of antizyme 1 in mouse reduces ornithine decarboxylase activity without major changes in tissue polyamine homeostasis.

    abstract::Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pietilä M,Dhungana H,Uimari A,Sironen R,Alhonen L

    更新日期:2014-02-01 00:00:00

  • Consequences of gene targeting procedures for behavioural responses and morphological development of newborn mice.

    abstract::In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: van der Meer M,Baumans V,Hofhuis FM,Olivier B,van Zutphen BF

    更新日期:2001-10-01 00:00:00