Lysozyme transgenic goats' milk positively impacts intestinal cytokine expression and morphology.


:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consumption of milk from transgenic goats producing human lysozyme (hLZ) in their milk therefore has the potential to positively impact intestinal health. In order to investigate the effect of hLZ-containing milk on the inflammatory response, young pigs were fed pasteurized milk from hLZ or non-transgenic control goats and quantitative real-time PCR was performed to assess local expression of TNF-α, IL-8, and TGF-β1 in the small intestine. Histological changes were also investigated, specifically looking at villi width, length, crypt depth, and lamina propria thickness along with cell counts for intraepithelial lymphocytes and goblet cells. Significantly higher expression of anti-inflammatory cytokine TGF-β1 was seen in the ileum of pigs fed pasteurized milk containing hLZ (P = 0.0478), along with an increase in intraepithelial lymphocytes (P = 0.0255), and decrease in lamina propria thickness in the duodenum (P = 0.0001). Based on these results we conclude that consuming pasteurized milk containing hLZ does not induce an inflammatory response and improves the health of the small intestine in pigs.


Transgenic Res


Transgenic research


Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD




Has Abstract


2011-12-01 00:00:00












  • Detailed characterization of Mirafiori lettuce virus-resistant transgenic lettuce.

    abstract::Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kawazu Y,Fujiyama R,Noguchi Y,Kubota M,Ito H,Fukuoka H

    更新日期:2010-04-01 00:00:00

  • Transportability of confined field trial data for environmental risk assessment of genetically engineered plants: a conceptual framework.

    abstract::It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Garcia-Alonso M,Hendley P,Bigler F,Mayeregger E,Parker R,Rubinstein C,Satorre E,Solari F,McLean MA

    更新日期:2014-12-01 00:00:00

  • Transgenic tobacco expressing Pinellia ternata agglutinin confers enhanced resistance to aphids.

    abstract::Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang K

    更新日期:2003-12-01 00:00:00

  • Constitutive expression of antimicrobial peptide PR-39 in transgenic mice significantly enhances resistance to bacterial infection and promotes growth.

    abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

    更新日期:2018-10-01 00:00:00

  • A complex RARE is required for the majority of Nedd9 embryonic expression.

    abstract::Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Knutson DC,Clagett-Dame M

    更新日期:2015-02-01 00:00:00

  • A one-step gene amplification system for use in cultured mammalian cells and transgenic animals.

    abstract::Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Melton DW,Ketchen AM,Kind AJ,McEwan C,Paisley D,Selfridge J

    更新日期:2001-04-01 00:00:00

  • Progress in Drosophila genome manipulation.

    abstract::The introduction of cloned and manipulated genetic material into the germline of an experimental organism is one of the most powerful tools of modern biology. In the case of the fruit fly, Drosophila melanogaster, there is also an unparalleled range of sophisticated genetic tools to facilitate subsequent analysis. In ...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Sentry LW,Kaiser K

    更新日期:1995-05-01 00:00:00

  • Application of genome editing in farm animals: cattle.

    abstract::Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Van Eenennaam AL

    更新日期:2019-08-01 00:00:00

  • Cannabinoid receptor 1 promotes hepatic lipid accumulation and lipotoxicity through the induction of SREBP-1c expression in zebrafish.

    abstract::The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pai WY,Hsu CC,Lai CY,Chang TZ,Tsai YL,Her GM

    更新日期:2013-08-01 00:00:00

  • Use of a cryptic splice donor site in the chloramphenicol acetyltransferase (CAT)-SV40 small-t antigen cassette generates alternative transcripts in transgenic rats.

    abstract::The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Burke ZD,Wells T,Carter DA,Baler R

    更新日期:2000-02-01 00:00:00

  • A comparison of the germline potential of differently aged ES cell lines and their transfected descendants.

    abstract::The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Fedorov LM,Haegel-Kronenberger H,Hirchenhain J

    更新日期:1997-05-01 00:00:00

  • Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice.

    abstract::Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pan PW,Käyrä K,Leinonen J,Nissinen M,Parkkila S,Rajaniemi H

    更新日期:2011-06-01 00:00:00

  • Introduction and expression of the bacterial glyoxylate cycle genes in transgenic mice.

    abstract::The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC and malate synthase (MS; EC, is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Saini KS,Byrne CR,Leish Z,Pruss CA,Rigby NW,Brownlee AG,Nancarrow CD,Ward KA

    更新日期:1996-11-01 00:00:00

  • Hairpin RNA-mediated silencing of Plum pox virus P1 and HC-Pro genes for efficient and predictable resistance to the virus.

    abstract::We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Di Nicola-Negri E,Brunetti A,Tavazza M,Ilardi V

    更新日期:2005-12-01 00:00:00

  • An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants.

    abstract::A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaer...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Schöffl F,Schröder G,Kliem M,Rieping M

    更新日期:1993-03-01 00:00:00

  • Over-expression of OSRIP18 increases drought and salt tolerance in transgenic rice plants.

    abstract::Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

    更新日期:2012-08-01 00:00:00

  • Gene targeting, genome editing: from Dolly to editors.

    abstract::One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Tan W,Proudfoot C,Lillico SG,Whitelaw CB

    更新日期:2016-06-01 00:00:00

  • A plant transformation vector with a minimal T-DNA.

    abstract::Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Düring K

    更新日期:1994-03-01 00:00:00

  • Antisense expression of peach mildew resistance locus O (PpMlo1) gene confers cross-species resistance to powdery mildew in Fragaria x ananassa.

    abstract::Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Jiwan D,Roalson EH,Main D,Dhingra A

    更新日期:2013-12-01 00:00:00

  • Overexpression of the wheat FK506-binding protein 73 (FKBP73) and the heat-induced wheat FKBP77 in transgenic wheat reveals different functions of the two isoforms.

    abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kurek I,Stöger E,Dulberger R,Christou P,Breiman A

    更新日期:2002-08-01 00:00:00

  • Development of transgenic zooplankton Artemia as a bioreactor to produce exogenous protein.

    abstract::Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Chang SH,Lee BC,Chen YD,Lee YC,Tsai HJ

    更新日期:2011-10-01 00:00:00

  • Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana.

    abstract::Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ling KS,Zhu HY,Gonsalves D

    更新日期:2008-08-01 00:00:00

  • The effect of Bt-transgene introgression on plant growth and reproduction in wild Brassica juncea.

    abstract::This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

    更新日期:2015-06-01 00:00:00

  • Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds.

    abstract::Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

    更新日期:2010-12-01 00:00:00

  • The advances and perspectives of recombinant protein production in the silk gland of silkworm Bombyx mori.

    abstract::The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Xu H

    更新日期:2014-10-01 00:00:00

  • Activation-tagged tobacco mutants that are tolerant to antimicrotubular herbicides are cross-resistant to chilling stress.

    abstract::T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ahad A,Wolf J,Nick P

    更新日期:2003-10-01 00:00:00

  • Systemic overexpression of antizyme 1 in mouse reduces ornithine decarboxylase activity without major changes in tissue polyamine homeostasis.

    abstract::Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pietilä M,Dhungana H,Uimari A,Sironen R,Alhonen L

    更新日期:2014-02-01 00:00:00

  • Specific pathogen free conditions prevent transthyretin amyloidosis in mouse models.

    abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Inoue S,Ohta M,Li Z,Zhao G,Takaoka Y,Sakashita N,Miyakawa K,Takada K,Tei H,Suzuki M,Masuoka M,Sakaki Y,Takahashi K,Yamamura K

    更新日期:2008-10-01 00:00:00

  • Engineering the plant cell factory for secondary metabolite production.

    abstract::Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Verpoorte R,van der Heijden R,Memelink J

    更新日期:2000-01-01 00:00:00

  • Gene silencing-based disease resistance.

    abstract::The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Wassenegger M

    更新日期:2002-12-01 00:00:00