Abstract：
:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consumption of milk from transgenic goats producing human lysozyme (hLZ) in their milk therefore has the potential to positively impact intestinal health. In order to investigate the effect of hLZ-containing milk on the inflammatory response, young pigs were fed pasteurized milk from hLZ or non-transgenic control goats and quantitative real-time PCR was performed to assess local expression of TNF-α, IL-8, and TGF-β1 in the small intestine. Histological changes were also investigated, specifically looking at villi width, length, crypt depth, and lamina propria thickness along with cell counts for intraepithelial lymphocytes and goblet cells. Significantly higher expression of anti-inflammatory cytokine TGF-β1 was seen in the ileum of pigs fed pasteurized milk containing hLZ (P = 0.0478), along with an increase in intraepithelial lymphocytes (P = 0.0255), and decrease in lamina propria thickness in the duodenum (P = 0.0001). Based on these results we conclude that consuming pasteurized milk containing hLZ does not induce an inflammatory response and improves the health of the small intestine in pigs.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JDdoi
10.1007/s11248-011-9489-7subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1235-43issue
6eissn
0962-8819issn
1573-9368journal_volume
20pub_type
杂志文章abstract：:Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976173
更新日期：1993-11-01 00:00:00
abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9388-3
更新日期：2011-02-01 00:00:00
abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...
journal_title：Transgenic research
pub_type： 信件,评审
doi：10.1007/s11248-009-9297-5
更新日期：2010-02-01 00:00:00
abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9711-x
更新日期：2013-12-01 00:00:00
abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9828-6
更新日期：2015-02-01 00:00:00
abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9350-4
更新日期：2010-08-01 00:00:00
abstract：:To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF02528779
更新日期：1992-05-01 00:00:00
abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title：Transgenic research
pub_type： 杂志文章,meta分析
doi：10.1007/s11248-013-9757-9
更新日期：2014-04-01 00:00:00
abstract：:To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title：Transgenic research
pub_type： 信件
doi：10.1007/s11248-012-9605-3
更新日期：2012-12-01 00:00:00
abstract：:A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9954-4
更新日期：2016-10-01 00:00:00
abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9563-1
更新日期：2012-06-01 00:00:00
abstract：:Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9147-2
更新日期：2008-08-01 00:00:00
abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9905-5
更新日期：2015-12-01 00:00:00
abstract：:Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9943-7
更新日期：2016-08-01 00:00:00
abstract：:Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9831-y
更新日期：2015-02-01 00:00:00
abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9928-6
更新日期：2016-06-01 00:00:00
abstract：:Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9014-6
更新日期：2006-12-01 00:00:00
abstract：:Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-0021-4
更新日期：2006-08-01 00:00:00
abstract：:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-8293-7
更新日期：2005-10-01 00:00:00
abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-007-9083-1
更新日期：2007-06-01 00:00:00
abstract：:Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-2543-6
更新日期：2006-02-01 00:00:00
abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9942-8
更新日期：2016-08-01 00:00:00
abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013986627504
更新日期：2002-02-01 00:00:00
abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018423015014
更新日期：1997-09-01 00:00:00
abstract：:Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9800-5
更新日期：2014-08-01 00:00:00
abstract：:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9182-7
更新日期：2008-12-01 00:00:00
abstract：:Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008886629825
更新日期：1999-04-01 00:00:00
abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-006-0024-1
更新日期：2006-08-01 00:00:00
abstract：:Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9763-y
更新日期：2014-02-01 00:00:00
abstract：:In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1012244404020
更新日期：2001-10-01 00:00:00
