Abstract：
:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consumption of milk from transgenic goats producing human lysozyme (hLZ) in their milk therefore has the potential to positively impact intestinal health. In order to investigate the effect of hLZ-containing milk on the inflammatory response, young pigs were fed pasteurized milk from hLZ or non-transgenic control goats and quantitative real-time PCR was performed to assess local expression of TNF-α, IL-8, and TGF-β1 in the small intestine. Histological changes were also investigated, specifically looking at villi width, length, crypt depth, and lamina propria thickness along with cell counts for intraepithelial lymphocytes and goblet cells. Significantly higher expression of anti-inflammatory cytokine TGF-β1 was seen in the ileum of pigs fed pasteurized milk containing hLZ (P = 0.0478), along with an increase in intraepithelial lymphocytes (P = 0.0255), and decrease in lamina propria thickness in the duodenum (P = 0.0001). Based on these results we conclude that consuming pasteurized milk containing hLZ does not induce an inflammatory response and improves the health of the small intestine in pigs.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JDdoi
10.1007/s11248-011-9489-7subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1235-43issue
6eissn
0962-8819issn
1573-9368journal_volume
20pub_type
杂志文章abstract：:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0054-x
更新日期：2018-02-01 00:00:00
abstract：:Follicle-stimulating hormone (FSH) is a critical hormone regulating reproduction in mammals. Transgenic mice show that overexpression of FSH can improve female fecundity. Using a bacterial artificial chromosome (BAC) system and somatic cell nuclear transfer, we herein generated 67 Large White transgenic (TG) boars har...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9963-3
更新日期：2016-10-01 00:00:00
abstract：:Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0083-0
更新日期：2018-10-01 00:00:00
abstract：:The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin protein is efficiently synthesized and precisely regulated is an important aspect that has yet to be fully elu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00175-w
更新日期：2019-12-01 00:00:00
abstract：:The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9461-y
更新日期：2011-08-01 00:00:00
abstract：:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9182-7
更新日期：2008-12-01 00:00:00
abstract：:Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022904805028
更新日期：2003-04-01 00:00:00
abstract：:The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016374128479
更新日期：2002-08-01 00:00:00
abstract：:The ability to tightly control transgene expression in vivo provides an opportunity to determine the role of certain gene products at different times during development and/or in response to different stimuli. We have characterized and evaluated a tetracycline-responsive endothelial-specific binary system during mouse...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008837410485
更新日期：1998-11-01 00:00:00
abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0084-z
更新日期：2018-10-01 00:00:00
abstract：:The beta-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicologic...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1008832711805
更新日期：1998-05-01 00:00:00
abstract：:The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1024220023057
更新日期：2003-08-01 00:00:00
abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9350-4
更新日期：2010-08-01 00:00:00
abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9300-1
更新日期：2010-04-01 00:00:00
abstract：:A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016671513425
更新日期：2001-08-01 00:00:00
abstract：:Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9879-3
更新日期：2015-08-01 00:00:00
abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9441-2
更新日期：2011-06-01 00:00:00
abstract：:SULT2A8 is a male-predominant and liver-specific mouse cytosolic sulfotransferase (SULT) that sulfonates 7α-hydroxyl (7α-OH) bile acids in vitro. Sulfonation regulates bile acid homeostasis, which in turn regulates cholesterol and energy metabolism. Using the Sult2a8-heterozygous (HT) mouse model created earlier in ou...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00215-w
更新日期：2020-12-01 00:00:00
abstract：:As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-007-9122-y
更新日期：2007-12-01 00:00:00
abstract：:Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9978-9
更新日期：2017-02-01 00:00:00
abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976498
更新日期：1995-01-01 00:00:00
abstract：:We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF02513027
更新日期：1992-03-01 00:00:00
abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9568-9
更新日期：2012-08-01 00:00:00
abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9884-6
更新日期：2015-08-01 00:00:00
abstract：:There has been considerable interest in pursuing phospholamban as a putative therapeutic target for overcoming depressed calcium handling in human heart failure. Studies predominantly done in mice have shown that phospholamban is a key regulator of sarcoplasmic reticulum calcium cycling and cardiac function. However, ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9139-2
更新日期：2008-04-01 00:00:00
abstract：:The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9420-7
更新日期：2011-04-01 00:00:00
abstract：:Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9831-y
更新日期：2015-02-01 00:00:00
abstract：:Cre/LoxP mediated chromosomal engineering in embryonic stem (ES) cells has a variety of applications, including the creation of model systems for studying aneuploidy. Targeted meiotic recombination (TAMERE) was proposed as a high efficiency in vivo alternative to effect Cre-mediated recombination, in which Cre recombi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-0389-6
更新日期：2005-06-01 00:00:00
abstract：:The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9685-0
更新日期：2013-08-01 00:00:00
abstract：:Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016633410041
更新日期：2001-06-01 00:00:00
