Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.


:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.


Transgenic Res


Transgenic research


Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H




Has Abstract


2011-12-01 00:00:00












  • Transgenic tobacco expressing Pinellia ternata agglutinin confers enhanced resistance to aphids.

    abstract::Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang K

    更新日期:2003-12-01 00:00:00

  • Opportunities for manipulating the seed protein composition of wheat and barley in order to improve quality.

    abstract::Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis M

    更新日期:1994-01-01 00:00:00

  • Molecular-based environmental risk assessment of three varieties of genetically engineered cows.

    abstract::The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

    更新日期:2011-10-01 00:00:00

  • Tissue specific high level expression of a full length human endogenous retrovirus genome transgene, HERV-R, under control of its own promoter in rats.

    abstract::Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

    更新日期:2003-06-01 00:00:00

  • Efficient gene-targeting in rat embryonic stem cells by CRISPR/Cas and generation of human kynurenine aminotransferase II (KAT II) knock-in rat.

    abstract::The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Yamamoto S,Ooshima Y,Nakata M,Yano T,Nishimura N,Nishigaki R,Satomi Y,Matsumoto H,Matsumoto Y,Takeyama M

    更新日期:2015-12-01 00:00:00

  • Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT).

    abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami T

    更新日期:2016-08-01 00:00:00

  • Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds.

    abstract::Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

    更新日期:2010-12-01 00:00:00

  • Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants.

    abstract::A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Christensen AH,Quail PH

    更新日期:1996-05-01 00:00:00

  • Transgene transmission to progeny by oMt1a-oGH transgenic mice.

    abstract::Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Thomas AD,Murray JD,Oberbauer AM

    更新日期:2005-08-01 00:00:00

  • Overexpression of antimicrobial lytic peptides protects grapevine from Pierce's disease under greenhouse but not field conditions.

    abstract::Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Li ZT,Hopkins DL,Gray DJ

    更新日期:2015-10-01 00:00:00

  • Specific pathogen free conditions prevent transthyretin amyloidosis in mouse models.

    abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Inoue S,Ohta M,Li Z,Zhao G,Takaoka Y,Sakashita N,Miyakawa K,Takada K,Tei H,Suzuki M,Masuoka M,Sakaki Y,Takahashi K,Yamamura K

    更新日期:2008-10-01 00:00:00

  • Improving seed size, seed weight and seedling emergence in Camelina sativa by overexpressing the Atsob3-6 gene variant.

    abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Sharma Koirala P,Neff MM

    更新日期:2020-08-01 00:00:00

  • Genetically engineered livestock for biomedical models.

    abstract::To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Rogers CS

    更新日期:2016-06-01 00:00:00

  • Identification of transgenic mice by direct PCR analysis of lysates of epithelial cells obtained from the inner surface of the rectum.

    abstract::Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

    更新日期:1998-03-01 00:00:00

  • A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.

    abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

    journal_title:Transgenic research

    pub_type: 杂志文章,meta分析


    authors: Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

    更新日期:2014-04-01 00:00:00

  • A rapid and reliable PCR-based assay for gene transmission and sex determination in newborn transgenic mice.

    abstract::This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), t...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lavrovsky Y,Song CS,Chatterjee B,Roy AK

    更新日期:1998-07-01 00:00:00

  • Interleukin 2 promoter/enhancer controlled expression of a synthetic cecropin-class lytic peptide in transgenic mice and subsequent resistance to Brucella abortus.

    abstract::The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KL

    更新日期:1997-09-01 00:00:00

  • Skeletal defects in Osterix-Cre transgenic mice.

    abstract::Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Huang W,Olsen BR

    更新日期:2015-02-01 00:00:00

  • Overexpression of the wheat FK506-binding protein 73 (FKBP73) and the heat-induced wheat FKBP77 in transgenic wheat reveals different functions of the two isoforms.

    abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kurek I,Stöger E,Dulberger R,Christou P,Breiman A

    更新日期:2002-08-01 00:00:00

  • Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori.

    abstract::To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

    更新日期:2010-06-01 00:00:00

  • An in vivo analysis of transcriptional elements in the mouse alpha-myosin heavy chain gene promoter.

    abstract::During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Rindt H,Subramaniam A,Robbins J

    更新日期:1995-11-01 00:00:00

  • A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature.

    abstract::The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

    更新日期:2008-06-01 00:00:00

  • Human bcr-abl gene has a lethal effect on embryogenesis.

    abstract::The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

    更新日期:1991-12-01 00:00:00

  • The effect of Bt-transgene introgression on plant growth and reproduction in wild Brassica juncea.

    abstract::This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

    更新日期:2015-06-01 00:00:00

  • Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana.

    abstract::Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ling KS,Zhu HY,Gonsalves D

    更新日期:2008-08-01 00:00:00

  • Construction of a transgenic pig model overexpressing polycystic kidney disease 2 (PKD2) gene.

    abstract::Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

    更新日期:2013-08-01 00:00:00

  • Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C. beticola.

    abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

    更新日期:2004-06-01 00:00:00

  • Cannabinoid receptor 1 promotes hepatic lipid accumulation and lipotoxicity through the induction of SREBP-1c expression in zebrafish.

    abstract::The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pai WY,Hsu CC,Lai CY,Chang TZ,Tsai YL,Her GM

    更新日期:2013-08-01 00:00:00

  • Use of a cryptic splice donor site in the chloramphenicol acetyltransferase (CAT)-SV40 small-t antigen cassette generates alternative transcripts in transgenic rats.

    abstract::The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Burke ZD,Wells T,Carter DA,Baler R

    更新日期:2000-02-01 00:00:00

  • Partial correction of abnormal cardiac development in caspase-8-deficient mice by cardiomyocyte expression of p 35.

    abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

    更新日期:2005-10-01 00:00:00