Abstract:
:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura Hdoi
10.1007/s11248-011-9495-9subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1285-92issue
6eissn
0962-8819issn
1573-9368journal_volume
20pub_type
杂志文章abstract::The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9909-1
更新日期:2015-12-01 00:00:00
abstract::Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9637-8
更新日期:2013-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008972010351
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abstract::The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018446325492
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00
abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9014-6
更新日期:2006-12-01 00:00:00
abstract::Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used beta-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, o...
journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2007-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0083-0
更新日期:2018-10-01 00:00:00
abstract::Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9568-9
更新日期:2012-08-01 00:00:00
abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title:Transgenic research
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doi:10.1007/s11248-011-9563-1
更新日期:2012-06-01 00:00:00
abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9288-6
更新日期:2010-02-01 00:00:00
abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000034610.35724.04
更新日期:2004-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00175-w
更新日期:2019-12-01 00:00:00
abstract::Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9152-5
更新日期:2008-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016633410041
更新日期:2001-06-01 00:00:00
abstract::Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9585-8
更新日期:2012-10-01 00:00:00
abstract::In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1012244404020
更新日期:2001-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01980212
更新日期:1996-11-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9715-6
更新日期:2013-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9765-9
更新日期:2014-04-01 00:00:00
abstract::The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9884-6
更新日期:2015-08-01 00:00:00
abstract::Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1024267416522
更新日期:2003-08-01 00:00:00
abstract::Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1015678416921
更新日期:2002-06-01 00:00:00
abstract::In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976498
更新日期:1995-01-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9101-3
更新日期:2008-04-01 00:00:00
abstract::Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9686-z
更新日期:2013-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章,评审
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更新日期:2014-10-01 00:00:00
abstract::The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1021130127700
更新日期:2002-12-01 00:00:00