Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.

abstract：:Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...

journal_title：Transgenic research

authors： Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang K

更新日期：2003-12-01 00:00:00

abstract：:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...

journal_title：Transgenic research

authors： Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis M

更新日期：1994-01-01 00:00:00

abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

journal_title：Transgenic research

authors： Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

更新日期：2011-10-01 00:00:00

abstract：:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

journal_title：Transgenic research

authors： Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

更新日期：2003-06-01 00:00:00

abstract：:The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...

journal_title：Transgenic research

authors： Yamamoto S,Ooshima Y,Nakata M,Yano T,Nishimura N,Nishigaki R,Satomi Y,Matsumoto H,Matsumoto Y,Takeyama M

更新日期：2015-12-01 00:00:00

abstract：:Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....

journal_title：Transgenic research

authors： Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami T

更新日期：2016-08-01 00:00:00

abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

journal_title：Transgenic research

authors： Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

更新日期：2010-12-01 00:00:00

abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

journal_title：Transgenic research

authors： Christensen AH,Quail PH

更新日期：1996-05-01 00:00:00

abstract：:Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...

journal_title：Transgenic research

authors： Thomas AD,Murray JD,Oberbauer AM

更新日期：2005-08-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...

journal_title：Transgenic research

authors： Inoue S,Ohta M,Li Z,Zhao G,Takaoka Y,Sakashita N,Miyakawa K,Takada K,Tei H,Suzuki M,Masuoka M,Sakaki Y,Takahashi K,Yamamura K

更新日期：2008-10-01 00:00:00

abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

journal_title：Transgenic research

authors： Sharma Koirala P,Neff MM

更新日期：2020-08-01 00:00:00

abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

journal_title：Transgenic research

authors： Rogers CS

更新日期：2016-06-01 00:00:00

abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

journal_title：Transgenic research

authors： Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

更新日期：1998-03-01 00:00:00

abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

journal_title：Transgenic research

authors： Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

更新日期：2014-04-01 00:00:00

abstract：:This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), t...

journal_title：Transgenic research

authors： Lavrovsky Y,Song CS,Chatterjee B,Roy AK

更新日期：1998-07-01 00:00:00

abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...

journal_title：Transgenic research

authors： Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KL

更新日期：1997-09-01 00:00:00

abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...

journal_title：Transgenic research

authors： Huang W,Olsen BR

更新日期：2015-02-01 00:00:00

abstract：:The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...

journal_title：Transgenic research

authors： Kurek I,Stöger E,Dulberger R,Christou P,Breiman A

更新日期：2002-08-01 00:00:00

abstract：:To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

journal_title：Transgenic research

authors： Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

更新日期：2010-06-01 00:00:00

abstract：:During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...

journal_title：Transgenic research

authors： Rindt H,Subramaniam A,Robbins J

更新日期：1995-11-01 00:00:00

abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

journal_title：Transgenic research

authors： Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

更新日期：2008-06-01 00:00:00

abstract：:The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

journal_title：Transgenic research

authors： Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

更新日期：1991-12-01 00:00:00

abstract：:This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

journal_title：Transgenic research

authors： Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

更新日期：2015-06-01 00:00:00

abstract：:Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...

journal_title：Transgenic research

authors： Ling KS,Zhu HY,Gonsalves D

更新日期：2008-08-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

journal_title：Transgenic research

authors： He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

更新日期：2013-08-01 00:00:00

abstract：:Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

journal_title：Transgenic research

authors： Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

更新日期：2004-06-01 00:00:00

abstract：:The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...

journal_title：Transgenic research

authors： Pai WY,Hsu CC,Lai CY,Chang TZ,Tsai YL,Her GM

更新日期：2013-08-01 00:00:00

abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

journal_title：Transgenic research

authors： Burke ZD,Wells T,Carter DA,Baler R

更新日期：2000-02-01 00:00:00

abstract：:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

journal_title：Transgenic research

authors： Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

更新日期：2005-10-01 00:00:00