Abstract：
:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchlings produced by this technique were raised to sexual maturity and shown to contain the foreign DNA in their sperm. G1 offspring containing this DNA were also produced in roughly 20% of these cockerels. In the majority of cases the vector DNA disappeared from the G1 generation as they matured suggesting that in these cases it had been transmitted episomally.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Li Y,Behnam J,Simkiss Kdoi
10.1007/BF01976498subject
Has Abstractpub_date
1995-01-01 00:00:00pages
26-9issue
1eissn
0962-8819issn
1573-9368journal_volume
4pub_type
杂志文章abstract：:Japanese morning glory, Ipomoea nil, exhibits a variety of flower colours, except yellow, reflecting the accumulation of only trace amounts of carotenoids in the petals. In a previous study, we attributed this effect to the low expression levels of carotenogenic genes in the petals, but there may be other contributing...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0051-0
更新日期：2018-02-01 00:00:00
abstract：:Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9825-9
更新日期：2015-02-01 00:00:00
abstract：:We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9275-y
更新日期：2009-12-01 00:00:00
abstract：:Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016633410041
更新日期：2001-06-01 00:00:00
abstract：:To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF02528779
更新日期：1992-05-01 00:00:00
abstract：:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0054-x
更新日期：2018-02-01 00:00:00
abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title：Transgenic research
pub_type： 杂志文章,meta分析
doi：10.1007/s11248-013-9757-9
更新日期：2014-04-01 00:00:00
abstract：:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1023381819572
更新日期：2003-06-01 00:00:00
abstract：:Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be relate...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1026517212807
更新日期：2000-12-01 00:00:00
abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9388-3
更新日期：2011-02-01 00:00:00
abstract：:Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9943-7
更新日期：2016-08-01 00:00:00
abstract：:The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1021130127700
更新日期：2002-12-01 00:00:00
abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1024267416522
更新日期：2003-08-01 00:00:00
abstract：:Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/b:trag.0000034610.35724.04
更新日期：2004-06-01 00:00:00
abstract：:Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9014-6
更新日期：2006-12-01 00:00:00
abstract：:The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-014-9826-8
更新日期：2014-10-01 00:00:00
abstract：:The ability to tightly control transgene expression in vivo provides an opportunity to determine the role of certain gene products at different times during development and/or in response to different stimuli. We have characterized and evaluated a tetracycline-responsive endothelial-specific binary system during mouse...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008837410485
更新日期：1998-11-01 00:00:00
abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9350-4
更新日期：2010-08-01 00:00:00
abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9563-1
更新日期：2012-06-01 00:00:00
abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9905-5
更新日期：2015-12-01 00:00:00
abstract：:Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used beta-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, o...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9107-x
更新日期：2007-12-01 00:00:00
abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9117-8
更新日期：2008-06-01 00:00:00
abstract：:Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9763-y
更新日期：2014-02-01 00:00:00
abstract：:Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1015678416921
更新日期：2002-06-01 00:00:00
abstract：:Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9152-5
更新日期：2008-04-01 00:00:00
abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-007-9083-1
更新日期：2007-06-01 00:00:00
abstract：:We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalen...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008972010351
更新日期：2000-06-01 00:00:00
abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008998832552
更新日期：2001-01-01 00:00:00
abstract：:Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0083-0
更新日期：2018-10-01 00:00:00
abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-1773-y
更新日期：2005-12-01 00:00:00
