Abstract：
:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were found in amyloid-negative 7.2hMet30 mice. These results suggested that TTR amyloidogenesis required not only mutant TTR but also an additional factor (or factors) as an etiologic molecule. To determine the differences in serum proteome in amyloid-positive and amyloid-negative mice in the 7.2-hMet30 model, we used proteomic analyses and studied serum samples obtained from these mice. Hemopexin (HPX) and transferrin (Tf) were detected in the serum samples from amyloid-positive mice and were also found in amyloid deposits via immunohistochemistry, but serum samples from amyloid-negative mice did not contain HPX and Tf. These two proteins were also not detected in non-fibrillar TTR deposits. In addition, in silico analyses suggested that HPX and Tf facilitate destabilization of TTR secondary structures and misfolding of TTR. These results suggest that HPX and Tf may be associated with TTR amyloidogenesis after fibrillogenesis in vivo.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Ohta M,Sugano A,Hatano N,Sato H,Shimada H,Niwa H,Sakaeda T,Tei H,Sakaki Y,Yamamura KI,Takaoka Ydoi
10.1007/s11248-017-0054-xsubject
Has Abstractpub_date
2018-02-01 00:00:00pages
15-23issue
1eissn
0962-8819issn
1573-9368pii
10.1007/s11248-017-0054-xjournal_volume
27pub_type
杂志文章abstract：:Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1008966404981
更新日期：2000-01-01 00:00:00
abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title：Transgenic research
pub_type： 杂志文章,meta分析
doi：10.1007/s11248-013-9757-9
更新日期：2014-04-01 00:00:00
abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0084-z
更新日期：2018-10-01 00:00:00
abstract：:At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-3913-9
更新日期：2006-04-01 00:00:00
abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008998832552
更新日期：2001-01-01 00:00:00
abstract：:Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-1771-0
更新日期：2005-12-01 00:00:00
abstract：:The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9723-6
更新日期：2014-02-01 00:00:00
abstract：:This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9688-5
更新日期：2013-10-01 00:00:00
abstract：:Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9879-3
更新日期：2015-08-01 00:00:00
abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018423015014
更新日期：1997-09-01 00:00:00
abstract：:The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018446325492
更新日期：1997-05-01 00:00:00
abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9905-5
更新日期：2015-12-01 00:00:00
abstract：:We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-9587-x
更新日期：2004-10-01 00:00:00
abstract：:Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976173
更新日期：1993-11-01 00:00:00
abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-007-9083-1
更新日期：2007-06-01 00:00:00
abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018461201385
更新日期：1997-01-01 00:00:00
abstract：:Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0083-0
更新日期：2018-10-01 00:00:00
abstract：:We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9146-3
更新日期：2008-08-01 00:00:00
abstract：:Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9152-5
更新日期：2008-04-01 00:00:00
abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008928121846
更新日期：2000-02-01 00:00:00
abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9821-0
更新日期：2015-02-01 00:00:00
abstract：:A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013007329936
更新日期：2001-12-01 00:00:00
abstract：:Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9147-2
更新日期：2008-08-01 00:00:00
abstract：:We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022194120518
更新日期：2003-02-01 00:00:00
abstract：:The ability to tightly control transgene expression in vivo provides an opportunity to determine the role of certain gene products at different times during development and/or in response to different stimuli. We have characterized and evaluated a tetracycline-responsive endothelial-specific binary system during mouse...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008837410485
更新日期：1998-11-01 00:00:00
abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008824509988
更新日期：1998-03-01 00:00:00
abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...
journal_title：Transgenic research
pub_type： 信件,评审
doi：10.1007/s11248-009-9297-5
更新日期：2010-02-01 00:00:00
abstract：:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-4350-5
更新日期：2005-10-01 00:00:00
abstract：:The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9219-y
更新日期：2009-02-01 00:00:00
abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008915007271
更新日期：1999-06-01 00:00:00
