Abstract:
:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, we observed defects in heart formation associated with abdominal hemorrhage and cardiomyocyte cell death in caspase-8-deficient animals. In order to better understand the etiology of the cardiac defects and embryonic lethality in caspase-8-deficient mice, we crossed these mice with the p 35 transgenic animals. Although the newly generated mice still died in utero and exhibited some cardiac defects, cardiomyocyte apoptosis was suppressed and ventricular trabeculation was restored. Thus, cardiomyocyte expression of p 35 prevented cell death induced by staurosporine or caspase-8 deficiency. Additionally, our data suggest that caspase-8 plays multiple roles in cardiac development.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki Kdoi
10.1007/s11248-005-4350-5subject
Has Abstractpub_date
2005-10-01 00:00:00pages
593-604issue
5eissn
0962-8819issn
1573-9368journal_volume
14pub_type
杂志文章abstract::The introduction of cloned and manipulated genetic material into the germline of an experimental organism is one of the most powerful tools of modern biology. In the case of the fruit fly, Drosophila melanogaster, there is also an unparalleled range of sophisticated genetic tools to facilitate subsequent analysis. In ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/BF01968780
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1007/s11248-006-9014-6
更新日期:2006-12-01 00:00:00
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章,meta分析
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更新日期:2014-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01980202
更新日期:1996-11-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1021130127700
更新日期:2002-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9909-1
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journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-015-9899-z
更新日期:2015-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1008928121846
更新日期:2000-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9821-0
更新日期:2015-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9978-9
更新日期:2017-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11248-005-0389-6
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pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
abstract::It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000026072.01351.10
更新日期:2004-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0007-2
更新日期:2006-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11248-005-4349-y
更新日期:2005-08-01 00:00:00
abstract::Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...
journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00