Abstract:
:At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ipsilon (Hufnagel), tobacco hornworm (Manduca sexta L.), and false tobacco budworm (= corn earworm Helicoverpa zea (Boddie)) was significantly reduced at several sample dates for high peroxidase vs. wild type plants. These results parallel those of prior laboratory studies with caterpillars. The number of adult whiteflies (Trialeurodes vaporariorum (Westwood) per plant was significantly reduced on high peroxidase compared to wild type plants on most sample dates in both years. The number of plants with leaves containing >100 aphids (primarily Myzus persicae Sulzer) per leaf on high peroxidase plants was significantly lower that on wild type plants after an equivalent invasion period in both years. A significantly higher proportion of aphids were found dead on leaf five of high peroxidase compared to wild type plants at most sample dates in both years. These results indicate that high peroxidase plants have resistance to a wide range of insects, implicating this enzyme as a broad range resistance mechanism.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Dowd PF,Lagrimini LMdoi
10.1007/s11248-005-3913-9subject
Has Abstractpub_date
2006-04-01 00:00:00pages
197-204issue
2eissn
0962-8819issn
1573-9368journal_volume
15pub_type
杂志文章abstract::Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9828-6
更新日期:2015-02-01 00:00:00
abstract::The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9909-1
更新日期:2015-12-01 00:00:00
abstract::Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9715-6
更新日期:2013-12-01 00:00:00
abstract::To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-016-9928-6
更新日期:2016-06-01 00:00:00
abstract::The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9685-0
更新日期:2013-08-01 00:00:00
abstract::Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9114-y
更新日期:2008-06-01 00:00:00
abstract::Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1015678416921
更新日期:2002-06-01 00:00:00
abstract::The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0054-x
更新日期:2018-02-01 00:00:00
abstract::Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1024267416522
更新日期:2003-08-01 00:00:00
abstract::As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-007-9122-y
更新日期:2007-12-01 00:00:00
abstract::To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02528779
更新日期:1992-05-01 00:00:00
abstract::One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-016-9932-x
更新日期:2016-06-01 00:00:00
abstract::Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9014-6
更新日期:2006-12-01 00:00:00
abstract::Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9722-7
更新日期:2014-02-01 00:00:00
abstract::During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01973758
更新日期:1995-11-01 00:00:00
abstract::Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9709-4
更新日期:2013-10-01 00:00:00
abstract::Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thali...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9085-z
更新日期:2008-02-01 00:00:00
abstract::Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0012-7
更新日期:2017-06-01 00:00:00
abstract::The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-015-9899-z
更新日期:2015-10-01 00:00:00
abstract::While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9101-3
更新日期:2008-04-01 00:00:00
abstract::We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9275-y
更新日期:2009-12-01 00:00:00
abstract::Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00198-8
更新日期:2020-06-01 00:00:00
abstract::Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4349-y
更新日期:2005-08-01 00:00:00
abstract::Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9825-9
更新日期:2015-02-01 00:00:00
abstract::Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9044-0
更新日期:2007-08-01 00:00:00
abstract::Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-007-9083-1
更新日期:2007-06-01 00:00:00
abstract::Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008998832552
更新日期:2001-01-01 00:00:00
abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016374128479
更新日期:2002-08-01 00:00:00
abstract::The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2), is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01980212
更新日期:1996-11-01 00:00:00
abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00208-9
更新日期:2020-08-01 00:00:00