Abstract:
:To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the human apolipoprotein A-I gene plus 10 kbp of its 5' flanking sequence and 1 kbp of its 3' flanking sequence. Both lines of transgenic rats express human apolipoprotein A-I mRNA in liver and human apolipoprotein A-I in plasma. Sera from these rats contain significantly higher levels of total apolipoprotein A-I, high density lipoprotein cholesterol and phospholipid than sera from non-transgenic littermates. Transgenic rats expressing high levels of human apolipoprotein A-I have reduced levels of serum rat apolipoprotein A-I suggesting a mechanism exists to down-regulate apolipoprotein A-I production. These transgenic rats provide a unique animal model to examine the effects of increased apolipoprotein A-I production on lipid and lipoprotein metabolism.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Swanson ME,Hughes TE,Denny IS,France DS,Paterniti JR Jr,Tapparelli C,Gfeller P,Bürki Kdoi
10.1007/BF02528779subject
Has Abstractpub_date
1992-05-01 00:00:00pages
142-7issue
3eissn
0962-8819issn
1573-9368journal_volume
1pub_type
杂志文章abstract::Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...
journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章,评审
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journal_title:Transgenic research
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pub_type: 杂志文章
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abstract::At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...
journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 信件,评审
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更新日期:2010-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9563-1
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9722-7
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1024267416522
更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章,meta分析
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:1996-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
abstract::Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1013986627504
更新日期:2002-02-01 00:00:00