Abstract:
:Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo can improve digestibility of dietary xylan and absorption of nutrients, we produced transgenic pigs which express the xynB from Aspergillus Niger CGMCC1067 in the parotid gland via nuclear transfer. In four live transgenic founders, β-xylanase activities in the saliva were 0.74, 0.59, 0.37 and 0.24 U/mL, respectively. Compared with non-transgenic pigs, the content of crude protein (CP) in feces reduced by 15.5% (P < 0.05). Furthermore, in 100 of the 271 F1 pigs the xynB gene was detectable. The digestibility of gross energy and CP in F1 transgenic pigs were increased by 5% and 22%, respectively, with the CP content in feces decreased by 6.4%. Taken together, our study showed that the transgenic pigs producing β-xylanase from parotid gland can reduce the anti-nutritional effect in animal diet and improve the utilization of nutrients.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Zhang M,Cai G,Zheng E,Zhang G,Li Y,Li Z,Yang H,Wu Zdoi
10.1007/s11248-019-00110-zsubject
Has Abstractpub_date
2019-04-01 00:00:00pages
189-198issue
2eissn
0962-8819issn
1573-9368pii
10.1007/s11248-019-00110-zjournal_volume
28pub_type
杂志文章abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title:Transgenic research
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journal_title:Transgenic research
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pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9715-6
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pub_type: 杂志文章
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章,评审
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5788-6
更新日期:2005-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016633410041
更新日期:2001-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0021-4
更新日期:2006-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0051-0
更新日期:2018-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4349-y
更新日期:2005-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000040053.23687.9c
更新日期:2004-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章,评审
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journal_title:Transgenic research
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journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
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journal_title:Transgenic research
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