Abstract:
:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patterns of OsMADS45 promoter was characterised by using the pOs45:GUS transgenic plants. Furthermore, the binary vector with Cre recombinase under the control of OsMADS45 promoter was constructed and introduced into rice by Agrobacterium-mediated transformation and transgenic rice plants were generated. Southern blot analysis showed that auto-excision of the selective markers occurred in some T(1) progeny of the transgenic plants, suggesting that a high auto-excision frequency can be achieved with our Cre/loxP system. This auto-excision strategy provides an efficient way of removing the selectable marker gene from transgenic rice.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Bai X,Wang Q,Chu Cdoi
10.1007/s11248-008-9182-7subject
Has Abstractpub_date
2008-12-01 00:00:00pages
1035-43issue
6eissn
0962-8819issn
1573-9368journal_volume
17pub_type
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journal_title:Transgenic research
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9114-y
更新日期:2008-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0083-0
更新日期:2018-10-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Transgenic research
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更新日期:2009-04-01 00:00:00
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journal_title:Transgenic research
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更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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