Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis.

abstract：:The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...

journal_title：Transgenic research

authors： Roberts A,Finardi-Filho F,Hegde S,Kiekebusch J,Klimpel G,Krieger M,Lema MA,Macdonald P,Nari C,Rubinstein C,Slutsky B,Vicien C

更新日期：2015-10-01 00:00:00

abstract：:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

journal_title：Transgenic research

authors： Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

更新日期：2011-12-01 00:00:00

abstract：:Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...

journal_title：Transgenic research

authors： Zurita E,Chagoyen M,Cantero M,Alonso R,González-Neira A,López-Jiménez A,López-Moreno JA,Landel CP,Benítez J,Pazos F,Montoliu L

更新日期：2011-06-01 00:00:00

abstract：:The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...

journal_title：Transgenic research

authors： Yamamoto S,Ooshima Y,Nakata M,Yano T,Nishimura N,Nishigaki R,Satomi Y,Matsumoto H,Matsumoto Y,Takeyama M

更新日期：2015-12-01 00:00:00

abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

journal_title：Transgenic research

authors： Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

更新日期：2011-10-01 00:00:00

abstract：:To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...

journal_title：Transgenic research

authors： Swanson ME,Hughes TE,Denny IS,France DS,Paterniti JR Jr,Tapparelli C,Gfeller P,Bürki K

更新日期：1992-05-01 00:00:00

abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

journal_title：Transgenic research

authors： Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

更新日期：1997-01-01 00:00:00

abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...

journal_title：Transgenic research

authors： Palaudelmàs M,Peñas G,Melé E,Serra J,Salvia J,Pla M,Nadal A,Messeguer J

更新日期：2009-08-01 00:00:00

abstract：:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...

journal_title：Transgenic research

authors： Garcia-Alonso M,Hendley P,Bigler F,Mayeregger E,Parker R,Rubinstein C,Satorre E,Solari F,McLean MA

更新日期：2014-12-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...

journal_title：Transgenic research

authors： Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS

更新日期：2011-12-01 00:00:00

abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...

journal_title：Transgenic research

authors： Yoshimura Y,Nakamura K,Endo T,Kajitani N,Kazuki K,Kazuki Y,Kugoh H,Oshimura M,Ohbayashi T

更新日期：2015-08-01 00:00:00

abstract：:Follicle-stimulating hormone (FSH) is a critical hormone regulating reproduction in mammals. Transgenic mice show that overexpression of FSH can improve female fecundity. Using a bacterial artificial chromosome (BAC) system and somatic cell nuclear transfer, we herein generated 67 Large White transgenic (TG) boars har...

journal_title：Transgenic research

authors： Xu P,Li Q,Jiang K,Yang Q,Bi M,Jiang C,Wang X,Wang C,Li L,Qiao C,Gong H,Xing Y,Ren J

更新日期：2016-10-01 00:00:00

abstract：:The ability to tightly control transgene expression in vivo provides an opportunity to determine the role of certain gene products at different times during development and/or in response to different stimuli. We have characterized and evaluated a tetracycline-responsive endothelial-specific binary system during mouse...

journal_title：Transgenic research

authors： Sarao R,Dumont DJ

更新日期：1998-11-01 00:00:00

abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

journal_title：Transgenic research

authors： Luo Y,Bolund L,Sørensen CB

更新日期：2012-06-01 00:00:00

abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

journal_title：Transgenic research

authors： Burke ZD,Wells T,Carter DA,Baler R

更新日期：2000-02-01 00:00:00

abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

journal_title：Transgenic research

authors： Ahad A,Wolf J,Nick P

更新日期：2003-10-01 00:00:00

abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...

journal_title：Transgenic research

authors： Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD

更新日期：2011-12-01 00:00:00

abstract：:Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...

journal_title：Transgenic research

authors： Suttiprapa S,Rinaldi G,Brindley PJ

更新日期：2012-06-01 00:00:00

abstract：:Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...

journal_title：Transgenic research

authors： Lu S,Seravalli J,Harrison-Findik D

更新日期：2015-08-01 00:00:00

abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...

journal_title：Transgenic research

authors： Ramessar K,Peremarti A,Gómez-Galera S,Naqvi S,Moralejo M,Muñoz P,Capell T,Christou P

更新日期：2007-06-01 00:00:00

abstract：:Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...

journal_title：Transgenic research

authors： Melton DW,Ketchen AM,Kind AJ,McEwan C,Paisley D,Selfridge J

更新日期：2001-04-01 00:00:00

abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...

journal_title：Transgenic research

authors： Di Nicola-Negri E,Brunetti A,Tavazza M,Ilardi V

更新日期：2005-12-01 00:00:00

abstract：:Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...

journal_title：Transgenic research

authors： Zheng SJ,Henken B,Sofiari E,Jacobsen E,Krens FA,Kik C

更新日期：2001-06-01 00:00:00

abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

journal_title：Transgenic research

authors： Heckart DL,Schwartz BM,Raymer PL,Parrott WA

更新日期：2016-08-01 00:00:00

abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

journal_title：Transgenic research

authors： Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

更新日期：2011-02-01 00:00:00

abstract：:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...

journal_title：Transgenic research

authors： Levin JS,Thompson WF,Csinos AS,Stephenson MG,Weissinger AK

更新日期：2005-04-01 00:00:00

abstract：:We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalen...

journal_title：Transgenic research

authors： Ko JH,Lee CS,Kim KH,Pang MG,Koo JS,Fang N,Koo DB,Oh KB,Youn WS,Zheng GD,Park JS,Kim SJ,Han YM,Choi IY,Lim J,Shin ST,Jin SW,Lee KK,Yoo OJ

更新日期：2000-06-01 00:00:00

abstract：:Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...

journal_title：Transgenic research

authors： Pavli OI,Panopoulos NJ,Goldbach R,Skaracis GN

更新日期：2010-10-01 00:00:00

abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

journal_title：Transgenic research

authors： Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

更新日期：2012-08-01 00:00:00

abstract：:The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....

journal_title：Transgenic research

authors： Wassenegger M

更新日期：2002-12-01 00:00:00