Hairpin RNA-mediated silencing of Plum pox virus P1 and HC-Pro genes for efficient and predictable resistance to the virus.


:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inverted repeats separated by an intron sequence under the transcriptional control of the Cauliflower Mosaic Virus 35S promoter. In a leaf disk infection assay, 38 out of 40 T0 transgenic plants were resistant to PPV infection. Eight lines, 2 for each construct, randomly selected among the 38 resistant plants were further analysed. Two hundred forty eight out of 253 T1 transgenic plants were resistant to local and systemic PPV infection. All transgenic single locus lines were completely resistant. These data indicate that the RNA silencing of PPV P1/HCPro sequences results in an efficient and predictable PPV resistance, which may be utilized in obtaining stone fruit plants resistant to the devastating Sharka disease.


Transgenic Res


Transgenic research


Di Nicola-Negri E,Brunetti A,Tavazza M,Ilardi V




Has Abstract


2005-12-01 00:00:00












  • Marking transgenic Xenopus froglets with passive micro transponders.

    abstract::Standard methods to mark Xenopus laevis individuals like tattooing or clipping toenails are inappropriate for the fast growing and regenerating small froglets and the previously used transponders are too large. In this study we successfully adapted micro transponders to tag these animals. Using these new transponders ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Waldner C,Roose M,Ryffel GU

    更新日期:2007-08-01 00:00:00

  • A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature.

    abstract::The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

    更新日期:2008-06-01 00:00:00

  • Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse.

    abstract::Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

    更新日期:2010-08-01 00:00:00

  • Production of two vaccinating recombinant rotavirus proteins in the milk of transgenic rabbits.

    abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Soler E,Le Saux A,Guinut F,Passet B,Cohen R,Merle C,Charpilienne A,Fourgeux C,Sorel V,Piriou A,Schwartz-Cornil I,Cohen J,Houdebine LM

    更新日期:2005-12-01 00:00:00

  • Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications.

    abstract::Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Zhang D,Corlet A,Fouilloux S

    更新日期:2008-06-01 00:00:00

  • Type II fish antifreeze protein accumulation in transgenic tobacco does not confer frost resistance.

    abstract::Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kenward KD,Brandle J,McPherson J,Davies PL

    更新日期:1999-04-01 00:00:00

  • Bovine alpha s1-casein gene sequences direct high level expression of human granulocyte-macrophage colony-stimulating factor in the milk of transgenic mice.

    abstract::The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

    更新日期:1997-01-01 00:00:00

  • Environmental impact of herbicide regimes used with genetically modified herbicide-resistant maize.

    abstract::With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Devos Y,Cougnon M,Vergucht S,Bulcke R,Haesaert G,Steurbaut W,Reheul D

    更新日期:2008-12-01 00:00:00

  • High level expression of human apolipoprotein A-I in transgenic rats raises total serum high density lipoprotein cholesterol and lowers rat apolipoprotein A-I.

    abstract::To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Swanson ME,Hughes TE,Denny IS,France DS,Paterniti JR Jr,Tapparelli C,Gfeller P,Bürki K

    更新日期:1992-05-01 00:00:00

  • Consequences of gene targeting procedures for behavioural responses and morphological development of newborn mice.

    abstract::In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: van der Meer M,Baumans V,Hofhuis FM,Olivier B,van Zutphen BF

    更新日期:2001-10-01 00:00:00

  • Regulation of human protein C gene expression by the mouse WAP promoter.

    abstract::A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

    更新日期:1994-11-01 00:00:00

  • Overexpression of the wheat FK506-binding protein 73 (FKBP73) and the heat-induced wheat FKBP77 in transgenic wheat reveals different functions of the two isoforms.

    abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Kurek I,Stöger E,Dulberger R,Christou P,Breiman A

    更新日期:2002-08-01 00:00:00

  • Availability of subfertile transgenic rats expressing the c-myc gene as recipients for spermatogonial transplantation.

    abstract::The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hirabayashi M,Yoshizawa Y,Kato M,Tsuchiya T,Nagao S,Hochi S

    更新日期:2009-02-01 00:00:00

  • Male-biased fasting-induced changes in hepatic tauro-cholic acid and plasma cholesterol in Sult2a8-haplodeficient mice.

    abstract::SULT2A8 is a male-predominant and liver-specific mouse cytosolic sulfotransferase (SULT) that sulfonates 7α-hydroxyl (7α-OH) bile acids in vitro. Sulfonation regulates bile acid homeostasis, which in turn regulates cholesterol and energy metabolism. Using the Sult2a8-heterozygous (HT) mouse model created earlier in ou...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Wang K,Chan MY,Xu J,Li PS,Liu X,Lee AY,Lee SS,Cheung WT

    更新日期:2020-12-01 00:00:00

  • In vivo characterization of plant promoter element interaction using synthetic promoters.

    abstract::Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Cazzonelli CI,Velten J

    更新日期:2008-06-01 00:00:00

  • Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis.

    abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pai CW,Chen YH

    更新日期:2010-08-01 00:00:00

  • Evaluation of the potential for interspecific hybridization between Camelina sativa and related wild Brassicaceae in anticipation of field trials of GM camelina.

    abstract::Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Julié-Galau S,Bellec Y,Faure JD,Tepfer M

    更新日期:2014-02-01 00:00:00

  • Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.

    abstract::The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

    更新日期:2011-12-01 00:00:00

  • Transgenic rice expressing the cry2AX1 gene confers resistance to multiple lepidopteran pests.

    abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Chakraborty M,Reddy PS,Mustafa G,Rajesh G,Narasu VM,Udayasuriyan V,Rana D

    更新日期:2016-10-01 00:00:00

  • Improved soybean transformation for efficient and high throughput transgenic production.

    abstract::Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Pareddy D,Chennareddy S,Anthony G,Sardesai N,Mall T,Minnicks T,Karpova O,Clark L,Griffin D,Bishop B,Shumway N,Samuel P,Smith K,Sarria R

    更新日期:2020-06-01 00:00:00

  • Improved paper pulp from plants with suppressed cinnamoyl-CoA reductase or cinnamyl alcohol dehydrogenase.

    abstract::Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

    更新日期:2002-10-01 00:00:00

  • Proposed criteria for identifying GE crop plants that pose a low or negligible risk to the environment under conditions of low-level presence in seed.

    abstract::The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审


    authors: Roberts A,Finardi-Filho F,Hegde S,Kiekebusch J,Klimpel G,Krieger M,Lema MA,Macdonald P,Nari C,Rubinstein C,Slutsky B,Vicien C

    更新日期:2015-10-01 00:00:00

  • Canadian regulatory aspects of gene editing technologies.

    abstract::The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Ellens KW,Levac D,Pearson C,Savoie A,Strand N,Louter J,Tibelius C

    更新日期:2019-08-01 00:00:00

  • Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters.

    abstract::Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Burket CT,Montgomery JE,Thummel R,Kassen SC,LaFave MC,Langenau DM,Zon LI,Hyde DR

    更新日期:2008-04-01 00:00:00

  • A germline-competent embryonic stem cell line from NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ (NSG) mice.

    abstract::The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Landel CP,Dunlap J,Patton JB,Manser T

    更新日期:2013-02-01 00:00:00

  • Cis effect of lacZ sequences in transgenic mice.

    abstract::Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Paldi A,Deltour L,Jami J

    更新日期:1993-11-01 00:00:00

  • Skeletal defects in Osterix-Cre transgenic mice.

    abstract::Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Huang W,Olsen BR

    更新日期:2015-02-01 00:00:00

  • Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (Oreochromis niloticus).

    abstract::Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be relate...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Rahman MA,Hwang GL,Razak SA,Sohm F,Maclean N

    更新日期:2000-12-01 00:00:00

  • Ex-post assessment of genetically modified, low level presence in Canadian flax.

    abstract::Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Booker HM,Lamb EG,Smyth SJ

    更新日期:2017-06-01 00:00:00

  • Inductively coupled mass spectrometry analysis of biometals in conditional Hamp1 and Hamp1 and Hamp2 transgenic mouse models.

    abstract::Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...

    journal_title:Transgenic research

    pub_type: 杂志文章


    authors: Lu S,Seravalli J,Harrison-Findik D

    更新日期:2015-08-01 00:00:00