Biosafety of E. coli beta-glucuronidase (GUS) in plants.

abstract：:It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

journal_title：Transgenic research

authors： Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

更新日期：2004-04-01 00:00:00

abstract：:Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...

journal_title：Transgenic research

authors： Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang K

更新日期：2003-12-01 00:00:00

abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...

journal_title：Transgenic research

authors： Zhang D,Corlet A,Fouilloux S

更新日期：2008-06-01 00:00:00

abstract：:We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...

journal_title：Transgenic research

authors： Petrie JR,Shrestha P,Belide S,Mansour MP,Liu Q,Horne J,Nichols PD,Singh SP

更新日期：2012-02-01 00:00:00

abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...

journal_title：Transgenic research

authors： Kawazu Y,Fujiyama R,Noguchi Y,Kubota M,Ito H,Fukuoka H

更新日期：2010-04-01 00:00:00

abstract：:The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

journal_title：Transgenic research

authors： Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

更新日期：1991-12-01 00:00:00

abstract：:The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...

journal_title：Transgenic research

authors： Allegra S,Bouazza L,Benetollo C,Li JY,Langlois D

更新日期：2005-02-01 00:00:00

abstract：:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

journal_title：Transgenic research

authors： Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

更新日期：1994-11-01 00:00:00

abstract：:The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...

journal_title：Transgenic research

authors： Carstens M,Vivier MA,Pretorius IS

更新日期：2003-08-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...

journal_title：Transgenic research

authors： Yamamoto S,Ooshima Y,Nakata M,Yano T,Nishimura N,Nishigaki R,Satomi Y,Matsumoto H,Matsumoto Y,Takeyama M

更新日期：2015-12-01 00:00:00

abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

journal_title：Transgenic research

authors： Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

更新日期：1997-01-01 00:00:00

abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...

journal_title：Transgenic research

authors： Sakamoto T

更新日期：2006-08-01 00:00:00

abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...

journal_title：Transgenic research

authors： Pan PW,Käyrä K,Leinonen J,Nissinen M,Parkkila S,Rajaniemi H

更新日期：2011-06-01 00:00:00

abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

journal_title：Transgenic research

authors： Heckart DL,Schwartz BM,Raymer PL,Parrott WA

更新日期：2016-08-01 00:00:00

abstract：:This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), t...

journal_title：Transgenic research

authors： Lavrovsky Y,Song CS,Chatterjee B,Roy AK

更新日期：1998-07-01 00:00:00

abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

journal_title：Transgenic research

authors： Luo Y,Bolund L,Sørensen CB

更新日期：2012-06-01 00:00:00

abstract：:Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be relate...

journal_title：Transgenic research

authors： Rahman MA,Hwang GL,Razak SA,Sohm F,Maclean N

更新日期：2000-12-01 00:00:00

abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

journal_title：Transgenic research

authors： Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

更新日期：2018-10-01 00:00:00

abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

journal_title：Transgenic research

authors： Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

更新日期：2010-12-01 00:00:00

abstract：:Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...

journal_title：Transgenic research

authors： Chang SH,Lee BC,Chen YD,Lee YC,Tsai HJ

更新日期：2011-10-01 00:00:00

abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

journal_title：Transgenic research

authors： Rogers CS

更新日期：2016-06-01 00:00:00

abstract：:The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...

journal_title：Transgenic research

authors： Xu H

更新日期：2014-10-01 00:00:00

abstract：:Phytocystatins are proteinaceous inhibitors of cysteine proteases. They have been implicated in the regulation of plant protein turnover and in defense against pathogens and insects. Here, we have characterized an Arabidopsis phytocystatin family gene, Arabidopsis thaliana phytocystatin 4 (AtCYS4). AtCYS4 was induced ...

journal_title：Transgenic research

authors： Je J,Song C,Hwang JE,Chung WS,Lim CO

更新日期：2014-02-01 00:00:00

abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

journal_title：Transgenic research

authors： Zbikowska HM

更新日期：2003-08-01 00:00:00

abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

journal_title：Transgenic research

authors： Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

更新日期：2014-04-01 00:00:00

abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...

journal_title：Transgenic research

authors： Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KL

更新日期：1997-09-01 00:00:00

abstract：:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...

journal_title：Transgenic research

authors： Garcia-Alonso M,Hendley P,Bigler F,Mayeregger E,Parker R,Rubinstein C,Satorre E,Solari F,McLean MA

更新日期：2014-12-01 00:00:00

abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...

journal_title：Transgenic research

authors： Jan FJ,Pang SZ,Fagoaga C,Gonsalves D

更新日期：1999-06-01 00:00:00

abstract：:RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...

journal_title：Transgenic research

authors： Xu L,Duan X,Lv Y,Zhang X,Nie Z,Xie C,Ni Z,Liang R

更新日期：2014-04-01 00:00:00