Abstract：
:Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be related to transgene copy number. Mosaic patterns of somatic lacZ expression were observed in these three lines which differed between lines but were consistent within a line. We also observed that expression of the reporter gene in homozygous transgenic fish was approximately two-fold greater than in the hemizygous transgenics. Analysis of expression of the reporter gene on a tissue-to-tissue basis demonstrated that lacZ expression of the reporter gene in stably transformed fish occured with variable intensity in different organs and tissues and was also sometimes variable in different cells of the same tissue in Gland G2 generations of the transgenic lines.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Rahman MA,Hwang GL,Razak SA,Sohm F,Maclean Ndoi
10.1023/a:1026517212807subject
Has Abstractpub_date
2000-12-01 00:00:00pages
417-27issue
6eissn
0962-8819issn
1573-9368journal_volume
9pub_type
杂志文章abstract：:Alkaloid concentration of perennial ryegrass herbage is affected by endophyte strain and host plant genotype. However, previous studies suggest that associations between host and endophyte also depends on environmental conditions, especially those affecting nutrient reserves and that water-soluble carbohydrate (WSC) c...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0087-9
更新日期：2018-10-01 00:00:00
abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1024267416522
更新日期：2003-08-01 00:00:00
abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title：Transgenic research
pub_type： 杂志文章,meta分析
doi：10.1007/s11248-013-9757-9
更新日期：2014-04-01 00:00:00
abstract：:Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9800-5
更新日期：2014-08-01 00:00:00
abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9563-1
更新日期：2012-06-01 00:00:00
abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01969712
更新日期：1996-05-01 00:00:00
abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9117-8
更新日期：2008-06-01 00:00:00
abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9942-8
更新日期：2016-08-01 00:00:00
abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9629-8
更新日期：2013-02-01 00:00:00
abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-1773-y
更新日期：2005-12-01 00:00:00
abstract：:Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01974093
更新日期：1994-03-01 00:00:00
abstract：:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-8293-7
更新日期：2005-10-01 00:00:00
abstract：:The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1015210919077
更新日期：2002-04-01 00:00:00
abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013986627504
更新日期：2002-02-01 00:00:00
abstract：:To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9328-2
更新日期：2010-06-01 00:00:00
abstract：:The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018446325492
更新日期：1997-05-01 00:00:00
abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9300-1
更新日期：2010-04-01 00:00:00
abstract：:Follicle-stimulating hormone (FSH) is a critical hormone regulating reproduction in mammals. Transgenic mice show that overexpression of FSH can improve female fecundity. Using a bacterial artificial chromosome (BAC) system and somatic cell nuclear transfer, we herein generated 67 Large White transgenic (TG) boars har...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-016-9963-3
更新日期：2016-10-01 00:00:00
abstract：:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-5413-8
更新日期：2005-04-01 00:00:00
abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00208-9
更新日期：2020-08-01 00:00:00
abstract：:Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9709-4
更新日期：2013-10-01 00:00:00
abstract：:Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/b:trag.0000034610.35724.04
更新日期：2004-06-01 00:00:00
abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9884-6
更新日期：2015-08-01 00:00:00
abstract：:Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1015678416921
更新日期：2002-06-01 00:00:00
abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9346-0
更新日期：2010-08-01 00:00:00
abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...
journal_title：Transgenic research
pub_type： 信件,评审
doi：10.1007/s11248-009-9297-5
更新日期：2010-02-01 00:00:00
abstract：:Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9763-y
更新日期：2014-02-01 00:00:00
abstract：:We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9275-y
更新日期：2009-12-01 00:00:00
abstract：:Standard methods to mark Xenopus laevis individuals like tattooing or clipping toenails are inappropriate for the fast growing and regenerating small froglets and the previously used transponders are too large. In this study we successfully adapted micro transponders to tag these animals. Using these new transponders ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9061-z
更新日期：2007-08-01 00:00:00
abstract：:Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00110-z
更新日期：2019-04-01 00:00:00
