A 7.1 kbp beta-myosin heavy chain promoter, efficient for green fluorescent protein expression, probably induces lethality when overexpressing a mutated transforming growth factor-beta type II receptor in transgenic mice.

abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...

journal_title：Transgenic research

authors： Huang W,Olsen BR

更新日期：2015-02-01 00:00:00

abstract：:In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...

journal_title：Transgenic research

authors： van der Meer M,Baumans V,Hofhuis FM,Olivier B,van Zutphen BF

更新日期：2001-10-01 00:00:00

abstract：:The introduction of cloned and manipulated genetic material into the germline of an experimental organism is one of the most powerful tools of modern biology. In the case of the fruit fly, Drosophila melanogaster, there is also an unparalleled range of sophisticated genetic tools to facilitate subsequent analysis. In ...

journal_title：Transgenic research

authors： Sentry LW,Kaiser K

更新日期：1995-05-01 00:00:00

abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...

journal_title：Transgenic research

authors： Di Nicola-Negri E,Brunetti A,Tavazza M,Ilardi V

更新日期：2005-12-01 00:00:00

abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

journal_title：Transgenic research

authors： Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

更新日期：2012-08-01 00:00:00

abstract：:Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vasc...

journal_title：Transgenic research

authors： Wang YA,Zheng JW,Fei ZL,Jiang XQ,Wang ZG,Fei J,Zhang ZY

更新日期：2009-04-01 00:00:00

abstract：:Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...

journal_title：Transgenic research

authors： Sivparsad BJ,Gubba A

更新日期：2014-04-01 00:00:00

abstract：:Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...

journal_title：Transgenic research

authors： Crowell EF,McGrath JM,Douches DS

更新日期：2008-04-01 00:00:00

abstract：:As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...

journal_title：Transgenic research

authors： Chapotin SM,Wolt JD

更新日期：2007-12-01 00:00:00

abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

journal_title：Transgenic research

authors： Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

更新日期：2010-12-01 00:00:00

abstract：:Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...

journal_title：Transgenic research

authors： Pavli OI,Panopoulos NJ,Goldbach R,Skaracis GN

更新日期：2010-10-01 00:00:00

abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...

journal_title：Transgenic research

authors： Li Y,Behnam J,Simkiss K

更新日期：1995-01-01 00:00:00

abstract：:Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...

journal_title：Transgenic research

authors： Booker HM,Lamb EG,Smyth SJ

更新日期：2017-06-01 00:00:00

abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

journal_title：Transgenic research

authors： Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

更新日期：2008-06-01 00:00:00

abstract：:We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...

journal_title：Transgenic research

authors： Peretz Y,Levy M,Avisar E,Edelbaum O,Rabinowitch H,Sela I

更新日期：2008-08-01 00:00:00

abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

journal_title：Transgenic research

authors： Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

更新日期：2011-02-01 00:00:00

abstract：:We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...

journal_title：Transgenic research

authors： Lamotte L,Jackerott M,Bucchini D,Jami J,Joshi RL,Deltour L

更新日期：2004-10-01 00:00:00

abstract：:Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...

journal_title：Transgenic research

authors： Verpoorte R,van der Heijden R,Memelink J

更新日期：2000-01-01 00:00:00

abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

journal_title：Transgenic research

authors： Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

更新日期：1998-03-01 00:00:00

abstract：:A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...

journal_title：Transgenic research

authors： Chakraborty M,Reddy PS,Mustafa G,Rajesh G,Narasu VM,Udayasuriyan V,Rana D

更新日期：2016-10-01 00:00:00

abstract：:Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...

journal_title：Transgenic research

authors： Patek CE,Saunders PT,Miles CG,Berry RL,Hastie ND,Sharpe RM,Hooper ML

更新日期：2005-10-01 00:00:00

abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

journal_title：Transgenic research

authors： Christensen AH,Quail PH

更新日期：1996-05-01 00:00:00

abstract：:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...

journal_title：Transgenic research

authors： Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis M

更新日期：1994-01-01 00:00:00

abstract：:Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...

journal_title：Transgenic research

authors： Tertivanidis K,Goudoula C,Vasilikiotis C,Hassiotou E,Perl-Treves R,Tsaftaris A

更新日期：2004-06-01 00:00:00

abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...

journal_title：Transgenic research

authors： Pareddy D,Chennareddy S,Anthony G,Sardesai N,Mall T,Minnicks T,Karpova O,Clark L,Griffin D,Bishop B,Shumway N,Samuel P,Smith K,Sarria R

更新日期：2020-06-01 00:00:00

abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...

journal_title：Transgenic research

authors： Ramessar K,Peremarti A,Gómez-Galera S,Naqvi S,Moralejo M,Muñoz P,Capell T,Christou P

更新日期：2007-06-01 00:00:00

abstract：:Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...

journal_title：Transgenic research

authors： Farré G,Perez-Fons L,Decourcelle M,Breitenbach J,Hem S,Zhu C,Capell T,Christou P,Fraser PD,Sandmann G

更新日期：2016-08-01 00:00:00

abstract：:The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...

journal_title：Transgenic research

authors： Hirabayashi M,Yoshizawa Y,Kato M,Tsuchiya T,Nagao S,Hochi S

更新日期：2009-02-01 00:00:00

abstract：:Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...

journal_title：Transgenic research

authors： Vellicce GR,Ricci JC,Hernández L,Castagnaro AP

更新日期：2006-02-01 00:00:00

abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...

journal_title：Transgenic research

authors： Rogers CS

更新日期：2016-06-01 00:00:00