Abstract:
:Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approaches were used to determine if increased levels of tocopherols can be accumulated in potato (Solanum tuberosum L.) tubers through metabolic engineering. Two transgenes were constitutively over-expressed in potato: Arabidopsis thaliana p-hydroxyphenylpyruvate dioxygenase (At-HPPD) and A. thaliana homogentisate phytyltransferase (At-HPT). alpha-Tocopherol levels in the transgenic plants were determined by high-performance liquid chromatography. In potato tubers, over-expression of At-HPPD resulted in a maximum 266% increase in alpha-tocopherol, and over-expression of At-HPT yielded a 106% increase. However, tubers from transgenic plants still accumulated approximately 10- and 100-fold less alpha-tocopherol than leaves or seeds, respectively. The results indicate that physiological and regulatory constraints may be the most limiting factors for tocopherol accumulation in potato tubers. Studying regulation and induction of tocopherol biosynthesis should reveal approaches to more effectively engineer crops with enhanced tocopherol content.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Crowell EF,McGrath JM,Douches DSdoi
10.1007/s11248-007-9091-1subject
Has Abstractpub_date
2008-04-01 00:00:00pages
205-17issue
2eissn
0962-8819issn
1573-9368journal_volume
17pub_type
杂志文章abstract::Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9388-3
更新日期:2011-02-01 00:00:00
abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-1771-0
更新日期:2005-12-01 00:00:00
abstract::It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9785-0
更新日期:2014-12-01 00:00:00
abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016374128479
更新日期:2002-08-01 00:00:00
abstract::Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-2543-6
更新日期:2006-02-01 00:00:00
abstract::Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1013986627504
更新日期:2002-02-01 00:00:00
abstract::Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00141-6
更新日期:2019-08-01 00:00:00
abstract::We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9517-7
更新日期:2012-02-01 00:00:00
abstract::The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-015-9899-z
更新日期:2015-10-01 00:00:00
abstract::Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9346-0
更新日期:2010-08-01 00:00:00
abstract::Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9709-4
更新日期:2013-10-01 00:00:00
abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9563-1
更新日期:2012-06-01 00:00:00
abstract::In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0039-9
更新日期:2017-12-01 00:00:00
abstract::Japanese morning glory, Ipomoea nil, exhibits a variety of flower colours, except yellow, reflecting the accumulation of only trace amounts of carotenoids in the petals. In a previous study, we attributed this effect to the low expression levels of carotenogenic genes in the petals, but there may be other contributing...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0051-0
更新日期:2018-02-01 00:00:00
abstract::We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalen...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008972010351
更新日期:2000-06-01 00:00:00
abstract::Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00198-8
更新日期:2020-06-01 00:00:00
abstract::The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9420-7
更新日期:2011-04-01 00:00:00
abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9954-4
更新日期:2016-10-01 00:00:00
abstract::Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9044-0
更新日期:2007-08-01 00:00:00
abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000034610.35724.04
更新日期:2004-06-01 00:00:00
abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00
abstract::Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1023381819572
更新日期:2003-06-01 00:00:00
abstract::Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/BF01976021
更新日期:1994-01-01 00:00:00
abstract::There has been considerable interest in pursuing phospholamban as a putative therapeutic target for overcoming depressed calcium handling in human heart failure. Studies predominantly done in mice have shown that phospholamban is a key regulator of sarcoplasmic reticulum calcium cycling and cardiac function. However, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9139-2
更新日期:2008-04-01 00:00:00
abstract::The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000040053.23687.9c
更新日期:2004-08-01 00:00:00
abstract::It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000026072.01351.10
更新日期:2004-04-01 00:00:00
abstract::To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-016-9928-6
更新日期:2016-06-01 00:00:00
abstract::Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1008966404981
更新日期:2000-01-01 00:00:00
abstract::Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9876-6
更新日期:2015-10-01 00:00:00
abstract::The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0054-x
更新日期:2018-02-01 00:00:00