A rapid and reliable PCR-based assay for gene transmission and sex determination in newborn transgenic mice.

Abstract:

:This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), the second set for the mouse Y-chromosome-specific SRY gene, and the third set for the beta subunit of the thyroid stimulating hormone (TSH beta), an internal control. This procedure allowed us to promptly analyze pups born from transgenic founders carrying the androgen receptor transgene and, at the same time, establish the sex of the animals. The method is simple, rapid and highly reproducible.

journal_name

Transgenic Res

journal_title

Transgenic research

authors

Lavrovsky Y,Song CS,Chatterjee B,Roy AK

doi

10.1023/a:1008838616203

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

319-20

issue

4

eissn

0962-8819

issn

1573-9368

journal_volume

7

pub_type

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