Abstract：
:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells from multicellular organisms but are toxic to a wide range of bacteria, protozoa and fungi, as well as infected and abnormal cells. Twenty-six lines of transgenic mice were produced by pronuclear injection of DNA consisting of the 5'-flanking region from -593 to +110 of the mouse interleukin 2 (IL-2) gene, Shiva 1a (a synthetic cecropinclass lytic peptide), and the SV40 polyadenylation/splice signal. A reverse-transcription PCR assay determined that two lines of transgenic mice were produced whose spleen-derived lymphocytes could be induced to transcribe and mature mRNA for Shiva 1a by exposure to 3.25 mg ml-1 of Con A. Two lines were challenged with an inoculation of 5 x 10(4) Brucella abortus strain 2308. After four weeks, there were significantly fewer B. abortus organisms in the spleens of transgenic mice than in non-transgenic control mice of the same strain (p < 0.05). Since the controlling regions of the IL-2 enhancer and the amino acid sequence of the signal peptide are highly conserved among several species, it is likely that this recombinant gene will function in other mammals.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KLdoi
10.1023/a:1018423015014subject
Has Abstractpub_date
1997-09-01 00:00:00pages
337-47issue
5eissn
0962-8819issn
1573-9368journal_volume
6pub_type
杂志文章abstract：:In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1012244404020
更新日期：2001-10-01 00:00:00
abstract：:We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9146-3
更新日期：2008-08-01 00:00:00
abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00208-9
更新日期：2020-08-01 00:00:00
abstract：:Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016633410041
更新日期：2001-06-01 00:00:00
abstract：:The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9685-0
更新日期：2013-08-01 00:00:00
abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1020362705497
更新日期：2002-10-01 00:00:00
abstract：:Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To furth...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9054-y
更新日期：2007-10-01 00:00:00
abstract：:Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/b:trag.0000005146.05655.7d
更新日期：2003-12-01 00:00:00
abstract：:Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9091-1
更新日期：2008-04-01 00:00:00
abstract：:The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9219-y
更新日期：2009-02-01 00:00:00
abstract：:To characterize the activity of the polyomavirus regulatory region, two hybrid marker genes were constructed. In the first construct, the early promoter regulates expression of the CAT gene and the late promoter regulates expression of the lacZ gene. In the second construct, the lacZ gene was placed under the control ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01980202
更新日期：1996-11-01 00:00:00
abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9250-7
更新日期：2009-08-01 00:00:00
abstract：:Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0083-0
更新日期：2018-10-01 00:00:00
abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title：Transgenic research
pub_type： 杂志文章,meta分析
doi：10.1007/s11248-013-9757-9
更新日期：2014-04-01 00:00:00
abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9477-3
更新日期：2011-10-01 00:00:00
abstract：:The introduction of cloned and manipulated genetic material into the germline of an experimental organism is one of the most powerful tools of modern biology. In the case of the fruit fly, Drosophila melanogaster, there is also an unparalleled range of sophisticated genetic tools to facilitate subsequent analysis. In ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/BF01968780
更新日期：1995-05-01 00:00:00
abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008928121846
更新日期：2000-02-01 00:00:00
abstract：:The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9723-6
更新日期：2014-02-01 00:00:00
abstract：:Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used beta-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, o...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9107-x
更新日期：2007-12-01 00:00:00
abstract：:Using the Sleeping Beauty (SB) transposon system, we have developed a simple method for the generation of Xenopus laevis transgenic lines. The transgenesis protocol is based on the co-injection of the SB transposase mRNA and a GFP-reporter transposon into one-cell stage embryos. Transposase-dependent reporter gene exp...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9014-6
更新日期：2006-12-01 00:00:00
abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9377-6
更新日期：2010-12-01 00:00:00
abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9821-0
更新日期：2015-02-01 00:00:00
abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1025814814823
更新日期：2003-10-01 00:00:00
abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9563-1
更新日期：2012-06-01 00:00:00
abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01969712
更新日期：1996-05-01 00:00:00
abstract：:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0054-x
更新日期：2018-02-01 00:00:00
abstract：:Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9879-3
更新日期：2015-08-01 00:00:00
abstract：:Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9556-0
更新日期：2012-06-01 00:00:00
abstract：:To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF02528779
更新日期：1992-05-01 00:00:00
abstract：:Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9637-8
更新日期：2013-04-01 00:00:00
