Introduction and expression of the bacterial glyoxylate cycle genes in transgenic mice.

abstract：:The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...

journal_title：Transgenic research

authors： Shin MR,Seo SG,Kim JS,Joen SB,Kang SW,Lee GP,Kwon SY,Kim SH

更新日期：2011-04-01 00:00:00

abstract：:Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a ...

journal_title：Transgenic research

authors： Cho YS,Lee SY,Kim DS,Nam YK

更新日期：2013-08-01 00:00:00

abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...

journal_title：Transgenic research

authors： Yoshimura Y,Nakamura K,Endo T,Kajitani N,Kazuki K,Kazuki Y,Kugoh H,Oshimura M,Ohbayashi T

更新日期：2015-08-01 00:00:00

abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

journal_title：Transgenic research

authors： Zbikowska HM

更新日期：2003-08-01 00:00:00

abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

journal_title：Transgenic research

authors： Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

更新日期：1998-03-01 00:00:00

abstract：:Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...

journal_title：Transgenic research

authors： Burket CT,Montgomery JE,Thummel R,Kassen SC,LaFave MC,Langenau DM,Zon LI,Hyde DR

更新日期：2008-04-01 00:00:00

abstract：:Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...

journal_title：Transgenic research

authors： Down RE,Ford L,Woodhouse SD,Davison GM,Majerus ME,Gatehouse JA,Gatehouse AM

更新日期：2003-04-01 00:00:00

abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

journal_title：Transgenic research

authors： Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

更新日期：1997-01-01 00:00:00

abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

journal_title：Transgenic research

authors： Christensen AH,Quail PH

更新日期：1996-05-01 00:00:00

abstract：:The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...

journal_title：Transgenic research

authors： Christeller JT,Burgess EP,Mett V,Gatehouse HS,Markwick NP,Murray C,Malone LA,Wright MA,Philip BA,Watt D,Gatehouse LN,Lövei GL,Shannon AL,Phung MM,Watson LM,Laing WA

更新日期：2002-04-01 00:00:00

abstract：:The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....

journal_title：Transgenic research

authors： Wassenegger M

更新日期：2002-12-01 00:00:00

abstract：:Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and...

journal_title：Transgenic research

authors： Nanto K,Ebinuma H

更新日期：2008-06-01 00:00:00

abstract：:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...

journal_title：Transgenic research

authors： Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis M

更新日期：1994-01-01 00:00:00

abstract：:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...

journal_title：Transgenic research

authors： Ohta M,Sugano A,Hatano N,Sato H,Shimada H,Niwa H,Sakaeda T,Tei H,Sakaki Y,Yamamura KI,Takaoka Y

更新日期：2018-02-01 00:00:00

abstract：:Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...

journal_title：Transgenic research

authors： Patek CE,Saunders PT,Miles CG,Berry RL,Hastie ND,Sharpe RM,Hooper ML

更新日期：2005-10-01 00:00:00

abstract：:The beta-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicologic...

journal_title：Transgenic research

authors： Gilissen LJ,Metz PL,Stiekema WJ,Nap JP

更新日期：1998-05-01 00:00:00

abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

journal_title：Transgenic research

authors： Burke ZD,Wells T,Carter DA,Baler R

更新日期：2000-02-01 00:00:00

abstract：:Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...

journal_title：Transgenic research

authors： Ashikawa I,Mori M,Nakamura S,Abe F

更新日期：2014-08-01 00:00:00

abstract：:Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...

journal_title：Transgenic research

authors： Paldi A,Deltour L,Jami J

更新日期：1993-11-01 00:00:00

abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...

journal_title：Transgenic research

authors： Hisatsune C,Ogawa N,Mikoshiba K

更新日期：2013-12-01 00:00:00

abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...

journal_title：Transgenic research

authors： Quijano CD,Brunner S,Keller B,Gruissem W,Sautter C

更新日期：2015-02-01 00:00:00

abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...

journal_title：Transgenic research

authors： Perl AK,Tichelaar JW,Whitsett JA

更新日期：2002-02-01 00:00:00

abstract：:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...

journal_title：Transgenic research

authors： Bai X,Wang Q,Chu C

更新日期：2008-12-01 00:00:00

abstract：:Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...

journal_title：Transgenic research

authors： Sivparsad BJ,Gubba A

更新日期：2014-04-01 00:00:00

abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...

journal_title：Transgenic research

authors： Li Y,Behnam J,Simkiss K

更新日期：1995-01-01 00:00:00

abstract：:The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...

journal_title：Transgenic research

authors： Cunha NB,Murad AM,Ramos GL,Maranhão AQ,Brígido MM,Araújo AC,Lacorte C,Aragão FJ,Covas DT,Fontes AM,Souza GH,Vianna GR,Rech EL

更新日期：2011-08-01 00:00:00

abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...

journal_title：Transgenic research

authors： Lizen B,Claus M,Jeannotte L,Rijli FM,Gofflot F

更新日期：2015-12-01 00:00:00

abstract：:While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...

journal_title：Transgenic research

authors： Zhang J,Cai L,Cheng J,Mao H,Fan X,Meng Z,Chan KM,Zhang H,Qi J,Ji L,Hong Y

更新日期：2008-04-01 00:00:00

abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

journal_title：Transgenic research

authors： Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

更新日期：2011-10-01 00:00:00

abstract：:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...

journal_title：Transgenic research

authors： Kopertekh L,Schiemann J

更新日期：2005-10-01 00:00:00