Long-range chromosomal engineering is more efficient in vitro than in vivo.

abstract：:A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformat...

journal_title：Transgenic research

authors： Skarjinskaia M,Svab Z,Maliga P

更新日期：2003-02-01 00:00:00

abstract：:Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...

journal_title：Transgenic research

authors： Khan SA,Zafar Y,Briddon RW,Malik KA,Mukhtar Z

更新日期：2006-06-01 00:00:00

abstract：:Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...

journal_title：Transgenic research

authors： Paldi A,Deltour L,Jami J

更新日期：1993-11-01 00:00:00

abstract：:The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

journal_title：Transgenic research

authors： Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

更新日期：1991-12-01 00:00:00

abstract：:Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...

journal_title：Transgenic research

authors： Farré G,Perez-Fons L,Decourcelle M,Breitenbach J,Hem S,Zhu C,Capell T,Christou P,Fraser PD,Sandmann G

更新日期：2016-08-01 00:00:00

abstract：:Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...

journal_title：Transgenic research

authors： Down RE,Ford L,Woodhouse SD,Davison GM,Majerus ME,Gatehouse JA,Gatehouse AM

更新日期：2003-04-01 00:00:00

abstract：:The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...

journal_title：Transgenic research

authors： Xu H

更新日期：2014-10-01 00:00:00

abstract：:The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...

journal_title：Transgenic research

authors： Allegra S,Bouazza L,Benetollo C,Li JY,Langlois D

更新日期：2005-02-01 00:00:00

abstract：:This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

journal_title：Transgenic research

authors： Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

更新日期：2015-06-01 00:00:00

abstract：:The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...

journal_title：Transgenic research

authors： Shin MR,Seo SG,Kim JS,Joen SB,Kang SW,Lee GP,Kwon SY,Kim SH

更新日期：2011-04-01 00:00:00

abstract：:To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

journal_title：Transgenic research

authors： Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

更新日期：2010-06-01 00:00:00

abstract：:The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...

journal_title：Transgenic research

authors： Streatfield SJ,Magallanes-Lundback ME,Beifuss KK,Brooks CA,Harkey RL,Love RT,Bray J,Howard JA,Jilka JM,Hood EE

更新日期：2004-08-01 00:00:00

abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

journal_title：Transgenic research

authors： Sharma Koirala P,Neff MM

更新日期：2020-08-01 00:00:00

abstract：:Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....

journal_title：Transgenic research

authors： Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami T

更新日期：2016-08-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

journal_title：Transgenic research

authors： Ahad A,Wolf J,Nick P

更新日期：2003-10-01 00:00:00

abstract：:Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...

journal_title：Transgenic research

authors： Inoue S,Ohta M,Li Z,Zhao G,Takaoka Y,Sakashita N,Miyakawa K,Takada K,Tei H,Suzuki M,Masuoka M,Sakaki Y,Takahashi K,Yamamura K

更新日期：2008-10-01 00:00:00

abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

journal_title：Transgenic research

authors： Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

更新日期：1997-01-01 00:00:00

abstract：:Mammalian Heat Shock Proteins (HSP), have potent immune-stimulatory properties due to the natural capability to associate with polypeptides and bind receptors on antigen presenting cells. The present study was aimed to explore whether plant HSP, and in particular HSP70, share similar properties. We wanted in particula...

journal_title：Transgenic research

authors： Buriani G,Mancini C,Benvenuto E,Baschieri S

更新日期：2011-04-01 00:00:00

abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...

journal_title：Transgenic research

authors： Li Y,Behnam J,Simkiss K

更新日期：1995-01-01 00:00:00

abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...

journal_title：Transgenic research

authors： Zhang XD,Letham DS,Zhang R,Higgins TJ

更新日期：1996-01-01 00:00:00

abstract：:The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...

journal_title：Transgenic research

authors： Yamamoto S,Ooshima Y,Nakata M,Yano T,Nishimura N,Nishigaki R,Satomi Y,Matsumoto H,Matsumoto Y,Takeyama M

更新日期：2015-12-01 00:00:00

abstract：:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...

journal_title：Transgenic research

authors： Garcia-Alonso M,Hendley P,Bigler F,Mayeregger E,Parker R,Rubinstein C,Satorre E,Solari F,McLean MA

更新日期：2014-12-01 00:00:00

abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...

journal_title：Transgenic research

authors： Cazzonelli CI,Velten J

更新日期：2008-06-01 00:00:00

abstract：:As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...

journal_title：Transgenic research

authors： Chapotin SM,Wolt JD

更新日期：2007-12-01 00:00:00

abstract：:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

journal_title：Transgenic research

authors： Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

更新日期：2003-06-01 00:00:00

abstract：:The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...

journal_title：Transgenic research

authors： Roberts A,Finardi-Filho F,Hegde S,Kiekebusch J,Klimpel G,Krieger M,Lema MA,Macdonald P,Nari C,Rubinstein C,Slutsky B,Vicien C

更新日期：2015-10-01 00:00:00

abstract：:While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...

journal_title：Transgenic research

authors： Zhang J,Cai L,Cheng J,Mao H,Fan X,Meng Z,Chan KM,Zhang H,Qi J,Ji L,Hong Y

更新日期：2008-04-01 00:00:00

abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

journal_title：Transgenic research

authors： Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

更新日期：2018-10-01 00:00:00

abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...

journal_title：Transgenic research

authors： Yoshimura Y,Nakamura K,Endo T,Kajitani N,Kazuki K,Kazuki Y,Kugoh H,Oshimura M,Ohbayashi T

更新日期：2015-08-01 00:00:00