Abstract：
:Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene constructs under control of a duplicate cauliflower mosaic virus 35S promoter, both with and without a native plant transit peptide sequence, were introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. AFP did not accumulate in the cytosol of transgenic plants, but active AFP was present as 2% the total protein present in the apoplast. Plant-produced AFP was the same size as mature Type II AFP isolated from fish, and was comparable to wild-type AFP in thermal hysteresis activity and its effect on ice crystal morphology. Field trials conducted in late summer on R1 generation transgenic plants showed similar AFP accumulation in plants under field conditions at levels suitable for large-scale production: but no difference in frost resistance was observed between transgenic and wild-type plants during the onset of early fall frosts.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Kenward KD,Brandle J,McPherson J,Davies PLdoi
10.1023/a:1008886629825subject
Has Abstractpub_date
1999-04-01 00:00:00pages
105-17issue
2eissn
0962-8819issn
1573-9368journal_volume
8pub_type
杂志文章abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9377-6
更新日期：2010-12-01 00:00:00
abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0084-z
更新日期：2018-10-01 00:00:00
abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9441-2
更新日期：2011-06-01 00:00:00
abstract：:Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9817-9
更新日期：2015-02-01 00:00:00
abstract：:To characterize the activity of the polyomavirus regulatory region, two hybrid marker genes were constructed. In the first construct, the early promoter regulates expression of the CAT gene and the late promoter regulates expression of the lacZ gene. In the second construct, the lacZ gene was placed under the control ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01980202
更新日期：1996-11-01 00:00:00
abstract：:Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9722-7
更新日期：2014-02-01 00:00:00
abstract：:This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9858-0
更新日期：2015-06-01 00:00:00
abstract：:The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018446325492
更新日期：1997-05-01 00:00:00
abstract：:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9785-0
更新日期：2014-12-01 00:00:00
abstract：:The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-5788-6
更新日期：2005-02-01 00:00:00
abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...
journal_title：Transgenic research
pub_type： 信件,评审
doi：10.1007/s11248-009-9297-5
更新日期：2010-02-01 00:00:00
abstract：:Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1008966404981
更新日期：2000-01-01 00:00:00
abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9250-7
更新日期：2009-08-01 00:00:00
abstract：:In the fowl the primordial germ cells accumulate in the germinal crescent to the anterior of the two-day embryo. A simple ballistic device has been used to fire tungsten particles (mean diameter 1.5 microns) into this region. By coating these projectiles with vector DNA it is possible to transfect these cells. Hatchli...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976498
更新日期：1995-01-01 00:00:00
abstract：:Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9044-0
更新日期：2007-08-01 00:00:00
abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9686-z
更新日期：2013-08-01 00:00:00
abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9711-x
更新日期：2013-12-01 00:00:00
abstract：:Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0012-7
更新日期：2017-06-01 00:00:00
abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9114-y
更新日期：2008-06-01 00:00:00
abstract：:It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/b:trag.0000026072.01351.10
更新日期：2004-04-01 00:00:00
abstract：:Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9825-9
更新日期：2015-02-01 00:00:00
abstract：:The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-015-9899-z
更新日期：2015-10-01 00:00:00
abstract：:Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To furth...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-9054-y
更新日期：2007-10-01 00:00:00
abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9489-7
更新日期：2011-12-01 00:00:00
abstract：:While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9101-3
更新日期：2008-04-01 00:00:00
abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9884-6
更新日期：2015-08-01 00:00:00
abstract：:With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9181-8
更新日期：2008-12-01 00:00:00
abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013986627504
更新日期：2002-02-01 00:00:00
abstract：:The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1021130127700
更新日期：2002-12-01 00:00:00
abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9156-1
更新日期：2008-06-01 00:00:00
