Abstract:
:Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To further improve the versatility of the two-hybrid EcR gene switch for wide spread use in plants, chimeras between Homo sapiens retinoid X receptor (HsRXR) and insect, Locusta migratoria RXR (LmRXR) were tested in tobacco protoplasts as partners with Choristoneura fumiferana EcR (CfEcR) in inducing expression of the luciferase reporter gene. The RXR chimera 9 (CH9) along with CfEcR, in a two-hybrid format gave the best results in terms of low-background expression levels in the absence of ligand and high-induced expression levels of the reporter gene in the presence of nanomolar concentrations of the methoxyfenozide ligand. The performance of CH9 was further tested in corn and soybean protoplasts and the data obtained was compared with the other EcR switches that contained the wild-type LmRXR or HsRXR as EcR partners. In both transient expression studies and stable transformation experiments, the fold induction values obtained with the CH9 switch were several times higher than the values obtained with the other EcR switches containing LmRXR or HsRXR. The new CfEcR two-hybrid gene switch that uses the RXR CH9 as a partner in inducing reporter gene expression provides an efficient, ligand-sensitive and tightly regulated gene switch for plants.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Tavva VS,Dinkins RD,Palli SR,Collins GBdoi
10.1007/s11248-006-9054-ysubject
Has Abstractpub_date
2007-10-01 00:00:00pages
599-612issue
5eissn
0962-8819issn
1573-9368journal_volume
16pub_type
杂志文章abstract::Japanese morning glory, Ipomoea nil, exhibits a variety of flower colours, except yellow, reflecting the accumulation of only trace amounts of carotenoids in the petals. In a previous study, we attributed this effect to the low expression levels of carotenogenic genes in the petals, but there may be other contributing...
journal_title:Transgenic research
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journal_title:Transgenic research
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pub_type: 信件,评审
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章,评审
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更新日期:2007-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9825-9
更新日期:2015-02-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9785-0
更新日期:2014-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9117-8
更新日期:2008-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0007-2
更新日期:2006-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9884-6
更新日期:2015-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9146-3
更新日期:2008-08-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9675-2
更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Transgenic research
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更新日期:2005-12-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9328-2
更新日期:2010-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-9587-x
更新日期:2004-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008915007271
更新日期:1999-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02513027
更新日期:1992-03-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0012-7
更新日期:2017-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9106-y
更新日期:2008-06-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000005146.05655.7d
更新日期:2003-12-01 00:00:00
abstract::Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9346-0
更新日期:2010-08-01 00:00:00