Characterization of stable fluorescent transgenic marine medaka (Oryzias dancena) lines carrying red fluorescent protein gene driven by myosin light chain 2 promoter.

abstract：:While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...

journal_title：Transgenic research

authors： Zhang J,Cai L,Cheng J,Mao H,Fan X,Meng Z,Chan KM,Zhang H,Qi J,Ji L,Hong Y

更新日期：2008-04-01 00:00:00

abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

journal_title：Transgenic research

authors： Burke ZD,Wells T,Carter DA,Baler R

更新日期：2000-02-01 00:00:00

abstract：:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

journal_title：Transgenic research

authors： Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

更新日期：2003-06-01 00:00:00

abstract：:Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...

journal_title：Transgenic research

authors： Lu S,Seravalli J,Harrison-Findik D

更新日期：2015-08-01 00:00:00

abstract：:Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...

journal_title：Transgenic research

authors： Ferry N,Mulligan EA,Stewart CN,Tabashnik BE,Port GR,Gatehouse AM

更新日期：2006-08-01 00:00:00

abstract：:Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vasc...

journal_title：Transgenic research

authors： Wang YA,Zheng JW,Fei ZL,Jiang XQ,Wang ZG,Fei J,Zhang ZY

更新日期：2009-04-01 00:00:00

abstract：:The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...

journal_title：Transgenic research

authors： Ellens KW,Levac D,Pearson C,Savoie A,Strand N,Louter J,Tibelius C

更新日期：2019-08-01 00:00:00

abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

journal_title：Transgenic research

authors： Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

更新日期：2018-10-01 00:00:00

abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...

journal_title：Transgenic research

authors： Lizen B,Claus M,Jeannotte L,Rijli FM,Gofflot F

更新日期：2015-12-01 00:00:00

abstract：:RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...

journal_title：Transgenic research

authors： Xu L,Duan X,Lv Y,Zhang X,Nie Z,Xie C,Ni Z,Liang R

更新日期：2014-04-01 00:00:00

abstract：:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...

journal_title：Transgenic research

authors： Bai X,Wang Q,Chu C

更新日期：2008-12-01 00:00:00

abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...

journal_title：Transgenic research

authors： Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD

更新日期：2011-12-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

journal_title：Transgenic research

authors： Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

更新日期：2011-12-01 00:00:00

abstract：:The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...

journal_title：Transgenic research

authors： Roberts A,Finardi-Filho F,Hegde S,Kiekebusch J,Klimpel G,Krieger M,Lema MA,Macdonald P,Nari C,Rubinstein C,Slutsky B,Vicien C

更新日期：2015-10-01 00:00:00

abstract：:A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...

journal_title：Transgenic research

authors： Nottle MB,Haskard KA,Verma PJ,Du ZT,Grupen CG,McIlfatrick SM,Ashman RJ,Harrison SJ,Barlow H,Wigley PL,Lyons IG,Cowan PJ,Crawford RJ,Tolstoshev PL,Pearse MJ,Robins AJ,d'Apice AJ

更新日期：2001-12-01 00:00:00

abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

journal_title：Transgenic research

authors： Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

更新日期：2010-12-01 00:00:00

abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...

journal_title：Transgenic research

authors： Pan PW,Käyrä K,Leinonen J,Nissinen M,Parkkila S,Rajaniemi H

更新日期：2011-06-01 00:00:00

abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

journal_title：Transgenic research

authors： Luo Y,Bolund L,Sørensen CB

更新日期：2012-06-01 00:00:00

abstract：:Cre/LoxP mediated chromosomal engineering in embryonic stem (ES) cells has a variety of applications, including the creation of model systems for studying aneuploidy. Targeted meiotic recombination (TAMERE) was proposed as a high efficiency in vivo alternative to effect Cre-mediated recombination, in which Cre recombi...

journal_title：Transgenic research

authors： Olson LE,Tien J,South S,Reeves RH

更新日期：2005-06-01 00:00:00

abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...

journal_title：Transgenic research

authors： Pareddy D,Chennareddy S,Anthony G,Sardesai N,Mall T,Minnicks T,Karpova O,Clark L,Griffin D,Bishop B,Shumway N,Samuel P,Smith K,Sarria R

更新日期：2020-06-01 00:00:00

abstract：:Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...

journal_title：Transgenic research

authors： Verpoorte R,van der Heijden R,Memelink J

更新日期：2000-01-01 00:00:00

abstract：:One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...

journal_title：Transgenic research

authors： Tan W,Proudfoot C,Lillico SG,Whitelaw CB

更新日期：2016-06-01 00:00:00

abstract：:Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...

journal_title：Transgenic research

authors： Zheng SJ,Henken B,Sofiari E,Jacobsen E,Krens FA,Kik C

更新日期：2001-06-01 00:00:00

abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

journal_title：Transgenic research

authors： Sharma Koirala P,Neff MM

更新日期：2020-08-01 00:00:00

abstract：:This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), t...

journal_title：Transgenic research

authors： Lavrovsky Y,Song CS,Chatterjee B,Roy AK

更新日期：1998-07-01 00:00:00

abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

journal_title：Transgenic research

authors： Ahad A,Wolf J,Nick P

更新日期：2003-10-01 00:00:00

abstract：:Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and ar...

journal_title：Transgenic research

authors： Lahm H,Hoeflich A,Rieger N,Wanke R,Wolf E

更新日期：1998-03-01 00:00:00

abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

journal_title：Transgenic research

authors： Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

更新日期：2010-08-01 00:00:00

abstract：:Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...

journal_title：Transgenic research

authors： Zurita E,Chagoyen M,Cantero M,Alonso R,González-Neira A,López-Jiménez A,López-Moreno JA,Landel CP,Benítez J,Pazos F,Montoliu L

更新日期：2011-06-01 00:00:00