Abstract：
:SULT2A8 is a male-predominant and liver-specific mouse cytosolic sulfotransferase (SULT) that sulfonates 7α-hydroxyl (7α-OH) bile acids in vitro. Sulfonation regulates bile acid homeostasis, which in turn regulates cholesterol and energy metabolism. Using the Sult2a8-heterozygous (HT) mouse model created earlier in our laboratory, we aimed to investigate the physiological role of SULT2A8 in sulfonating 7α-OH bile acids and its impact on energy metabolism in vivo under both fed and energy-deprivation conditions. Disruption of one allele of the Sult2a8 gene in male HT mice resulted in losing ~ 50% of the 7α-OH sulfonating activity compared to wild-type (WT) control, but no significant change in female HT mice. Under the fed condition comparing the levels of hepatic and biliary bile acids as well as plasma/serum energy metabolites, HT mice displayed a profile similar to that of WT mice, suggesting that the Sult2a8-haplodeficient mice conducted normal energy metabolism. However, after 48-h fasting, a significant decrease in plasma cholesterol level was found in male HT mice but without any significant reduction in female HT mice. Of interest, in male Sult2a8-haplodeficient mice, an increase of the hepatic taurine-conjugated cholic acid level was noted but no noticeable change in other tested bile acids after fasting. Taken together, SULT2A8 is a male-specific and key hepatic SULT in metabolizing 7α-OH primary bile acids. During energy deprivation, SULT2A8 is required to maintain the bile acid and cholesterol metabolism, suggesting SULT is a potential therapeutic target for controlling metabolic diseases.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Wang K,Chan MY,Xu J,Li PS,Liu X,Lee AY,Lee SS,Cheung WTdoi
10.1007/s11248-020-00215-wsubject
Has Abstractpub_date
2020-12-01 00:00:00pages
499-510issue
5-6eissn
0962-8819issn
1573-9368pii
10.1007/s11248-020-00215-wjournal_volume
29pub_type
杂志文章abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1020362705497
更新日期：2002-10-01 00:00:00
abstract：:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0054-x
更新日期：2018-02-01 00:00:00
abstract：:We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9275-y
更新日期：2009-12-01 00:00:00
abstract：:Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-7216-y
更新日期：2005-10-01 00:00:00
abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...
journal_title：Transgenic research
pub_type： 信件,评审
doi：10.1007/s11248-009-9297-5
更新日期：2010-02-01 00:00:00
abstract：:Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9637-8
更新日期：2013-04-01 00:00:00
abstract：:To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF02528779
更新日期：1992-05-01 00:00:00
abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00198-8
更新日期：2020-06-01 00:00:00
abstract：:The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin protein is efficiently synthesized and precisely regulated is an important aspect that has yet to be fully elu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00175-w
更新日期：2019-12-01 00:00:00
abstract：:Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01974093
更新日期：1994-03-01 00:00:00
abstract：:Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patter...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9182-7
更新日期：2008-12-01 00:00:00
abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9828-6
更新日期：2015-02-01 00:00:00
abstract：:Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9152-5
更新日期：2008-04-01 00:00:00
abstract：:With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9181-8
更新日期：2008-12-01 00:00:00
abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9884-6
更新日期：2015-08-01 00:00:00
abstract：:We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-9587-x
更新日期：2004-10-01 00:00:00
abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00208-9
更新日期：2020-08-01 00:00:00
abstract：:Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-4349-y
更新日期：2005-08-01 00:00:00
abstract：:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-8293-7
更新日期：2005-10-01 00:00:00
abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9114-y
更新日期：2008-06-01 00:00:00
abstract：:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-5413-8
更新日期：2005-04-01 00:00:00
abstract：:Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9479-1
更新日期：2011-10-01 00:00:00
abstract：:Japanese morning glory, Ipomoea nil, exhibits a variety of flower colours, except yellow, reflecting the accumulation of only trace amounts of carotenoids in the petals. In a previous study, we attributed this effect to the low expression levels of carotenogenic genes in the petals, but there may be other contributing...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0051-0
更新日期：2018-02-01 00:00:00
abstract：:Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9180-9
更新日期：2008-10-01 00:00:00
abstract：:A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformat...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022110402302
更新日期：2003-02-01 00:00:00
abstract：:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9785-0
更新日期：2014-12-01 00:00:00
abstract：:Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9715-6
更新日期：2013-12-01 00:00:00
abstract：:The random germline integration of genetically engineered transgenes has been a powerful technique to study the role of particular genes in variety of biological processes. Although the identification of the transgene insertion site is often not essential for functional analysis of the transgene, identifying the site ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9190-7
更新日期：2008-10-01 00:00:00
abstract：:To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title：Transgenic research
pub_type： 信件
doi：10.1007/s11248-012-9605-3
更新日期：2012-12-01 00:00:00
abstract：:Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9831-y
更新日期：2015-02-01 00:00:00
