Abstract:
:This study was designed to test the possibility that antimicrobial peptides could be derived from the genomic sequences of phage lysins. Using two lysins (D3 and PhiKZ) we selected and produced two putative peptides (X and Z, respectively) believed to possess antimicrobial properties based on their physicochemical characteristics. The data presented support this hypothesis in that the peptides and various analogs displayed antibacterial activity, bacteriostatic or bactericidal, either individually or upon combination. These putative peptides are believed to act by a mechanism of action resembling that of conventional antimicrobial peptides when judged by both structural and functional criteria. Thus, the peptides are shown to have the ability to form a helical structure, to bind to model bacterial membranes and permeabilize model liposomes. They also display rapid bactericidal kinetics and their antibacterial potency is increased upon amidation. The possible relevance of these results in contributing to potency of phage lysins is discussed. Such peptides may be used to design new potent antimicrobial compounds much needed in face of the ever threatening drug resistance problems.
journal_name
Peptidesjournal_title
Peptidesauthors
Rotem S,Radzishevsky I,Inouye RT,Samore M,Mor Adoi
10.1016/j.peptides.2005.07.001subject
Has Abstractpub_date
2006-01-01 00:00:00pages
18-26issue
1eissn
0196-9781issn
1873-5169pii
S0196-9781(05)00321-9journal_volume
27pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Neural input to distinct and separate populations of CRF-immunoreactive (ir) neurons in rat forebrain was investigated. The relationship of opiocortin and/or catecholamine fibers to different groups of CRF-containing neurons was elucidated using single and dual labeling immunocytochemical procedures. Antibodies to CRF...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90005-3
更新日期:1989-11-01 00:00:00
abstract::This study was performed to identify the efferents of the dorsal raphe nucleus (DRN), particularly in regard to nociception. Phaseolus vulgaris leucoagglutinin (PHA-L) was microiontophoresed into the DRN and visualized immunocytochemically; PHA-L-immunoreactive (-IR) fibers and terminals were identified in the forebra...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90013-7
更新日期:1993-01-01 00:00:00
abstract::Endothelin-1 may contribute to bronchial smooth muscle constriction and airway remodelling in asthma, where bronchial epithelial cells represent an important source of this peptide. We report here that asthmatic bronchial epithelial cells exposed to allergens in vitro induce the differentiation of airway fibroblasts i...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00194-0
更新日期:1997-01-01 00:00:00
abstract::Combined use of the intraaxonal retrograde transport of the fluorescent marker 'true blue' with substance P (SP) immunocytochemistry has been used to trace the nodose ganglion projections of SP-containing neurons of the aortic depressor nerve. It has been found that (1) SP immunoreactive (SP-I) cell bodies are clearly...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(80)90015-7
更新日期:1980-01-01 00:00:00
abstract::ZP120 is a nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP) ligand. In previous studies, the effects of ZP120 were found to be sensitive to J-113397 in mouse tissues while resistant to UFP-101 in rat tissues. The aim of this study was to further investigate the ZP120 pharmacological profile using mouse and rat pr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.10.001
更新日期:2009-02-01 00:00:00
abstract::Peptidomic analysis of norepinephrine-stimulated skin secretions of the South-East Asian frog Hylarana erythraea (formerly Rana erythraea partim) has led to the identification of multiple peptides with antimicrobial activity. Structural characterization of the peptides demonstrated that they belong to the brevinin-1 (...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.12.013
更新日期:2010-04-01 00:00:00
abstract::The pentapeptide pGlu-Asn-Cyt-Pro-Arg [AVP(4-8), termed ZNC(C)PR in this article] has been found as a metabolite of arginine-vasopressin (AVP) in rat brain and has been shown to have potent memory-enhancing activity and to yield a series of biochemical events in brain tissues. [35S]ZNC(C)PR was chemically synthesized ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90153-8
更新日期:1994-01-01 00:00:00
abstract::The in vitro interaction between five polycationic peptides, buforin II, cecropin P1, indolicidin, magainin II, and ranalexin, and several clinically used antimicrobial agents was evaluated against several clinical isolates of Gram-positive and Gram-negative aerobic bacteria, using the microbroth dilution method. The ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(00)00254-0
更新日期:2000-08-01 00:00:00
abstract::We are interested in the presence and function in insects of neuropeptides related to the vertebrate tachykinins. Hence, we have used antisera raised against the tachykinins substance P and kassinin, and against the insect neuropeptide leucokinin I, for localization studies and immunochemical analysis of related pepti...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90094-w
更新日期:1993-07-01 00:00:00
abstract::Skh-AMP1 (GRTSKQELCTWERGSVRQADKTIAG) is an antifungal peptide isolated from Satureja khuzistanica which has been shown to have strong antifungal activity against Aspergillus and Candida species, but no obvious hemolytic effects or cell cytotoxicity in vitro. In the present study, Skh-AMP1 was synthesized, and its mode...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2019.170195
更新日期:2020-01-01 00:00:00
abstract::Binding studies have shown that [125I]NKA is a selective ligand of tachykinin septide-sensitive binding sites from membranes of the rat submaxillary gland. Indeed, this ligand bound with high affinity to a single population of sites. In addition, competition studies indicated that natural tachykinins and tachykinin-re...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00140-0
更新日期:1999-11-01 00:00:00
abstract::Three novel antimicrobial peptides designated ToAMP1, ToAMP2 and ToAMP3 were purified from Taraxacum officinale flowers. Their amino acid sequences were determined. The peptides are cationic and cysteine-rich and consist of 38, 44 and 42 amino acid residues for ToAMP1, ToAMP2 and ToAMP3, respectively. Importantly, acc...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.05.009
更新日期:2012-08-01 00:00:00
abstract::Generally, biomacromolecules, such as DNA, RNA, and proteins, cannot freely permeate into cells from outside the membrane. Protein transduction domains (PTDs) are peptides containing a large number of basic amino acids that can deliver macromolecules into living cells. Arginine-rich intracellular delivery (AID) peptid...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.06.006
更新日期:2009-09-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP) is a neuropeptide present in high concentrations in the hippocampus. The studies reported here demonstrate that VIP administered into the third ventricle of the brain caused amnesia in mice trained on a left-right footshock avoidance task in a T-maze. VIP resulted in amnesia when ad...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90012-t
更新日期:1990-09-01 00:00:00
abstract::We have isolated TDVDHVFLRFamide (DMS), FDDYGHMRFamide (DSK), and DPKQDFMRFamide from Drosophila melanogaster. These peptides, structurally related by a common C-terminus -XRFamide, where X = L or M, are encoded by three different genes. To determine cellular expression, we have generated antisera to multiple antigeni...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(96)00279-3
更新日期:1997-01-01 00:00:00
abstract::This study evaluated the physiological importance of Angiotensin-(1-7) receptor Mas on reflex control of circulation. Experiments were performed in male Mas-knockout (Mas-KO) and Wild Type (WT) conscious mice (12-20 wk of age). Baroreceptor reflex was evaluated by the bradycardic response induced by phenylephrine (0.2...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.06.030
更新日期:2010-10-01 00:00:00
abstract::The distribution of corazonin in the central nervous system of the heteropteran insect Triatoma infestans was studied by immunohistochemistry. The presence of corazonin isoforms was investigated using MALDI-TOF mass spectrometry in samples containing the brain, the subesophageal ganglion, the corpora cardiaca-corpus a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.10.020
更新日期:2011-03-01 00:00:00
abstract::The effects of third ventricular (IVT) injection of 25 micrograms of bradykinin (BK) upon plasma levels of LH, FSH, TSH, GH and prolactin were investigated in conscious ovariectomized female rats bearing indwelling jugular cannulae. Some animals were pretreated with bradykinin potentiating factor (BPF). Intravenous ad...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(80)90054-6
更新日期:1980-10-01 00:00:00
abstract::Previous studies have shown that the nervous system and other tissues of molluscs contain a number of peptides that potently excite molluscan hearts. Two such peptides, termed small cardioactive peptides A and B (SCPA and SCPB) are present in large quantities in the nervous system of Aplysia. These peptides are widely...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90184-7
更新日期:1987-01-01 00:00:00
abstract::The effect of synthetic human calcitonin gene-related peptide (hCGRP) on the isolated and electrically driven left atria of rats were investigated. The peptide at concentrations of 3 X 10(-9)-3 X 10(-7) M produced positive inotropic effects on the left atria in a dose-dependent manner. Verapamil (10(-5) M) and adenosi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90038-0
更新日期:1986-07-01 00:00:00
abstract::Thirty viral proteomes were examined for amino acid sequence similarity to the human proteome, and, in parallel, a control of 30 sets of human proteins was analyzed for internal human overlapping. We find that all of the analyzed 30 viral proteomes, independently of their structural or pathogenic characteristics, pres...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.05.022
更新日期:2008-10-01 00:00:00
abstract::In the present review we reevaluated the experimental and clinical evidence that there is a local renin angiotensin system in the heart as well as the presence of a functional intracrine component which is activated during pathological conditions like heart failure and hypertension. The implications of these findings ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2011.06.018
更新日期:2011-08-01 00:00:00
abstract::Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (NPFF), an endogenous mammalian antiopioid peptide, has been shown by other laboratories to attenuate the acute antinociceptive effects of morphine, the development of morphine tolerance, and naloxone-induced withdrawal in morphine-dependent rats. The present study determined the ef...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90187-l
更新日期:1993-11-01 00:00:00
abstract::Atrial natriuretic peptide (ANP) is increasingly expressed on airway and inhibits pulmonary arterial remodeling. However, the role of ANP in remodeling of respiratory system is still unclear. The role of ANP on airway remodeling and the possible mechanism was explored in this study. Both human bronchial epithelial 16H...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2017.02.002
更新日期:2017-04-01 00:00:00
abstract::Dermorphin, injected intracerebroventricularly (ICV) to rats, provokes, like to morphine, an inhibition of intestinal propulsion linearly related to the log of the administered doses (in the range from 0.06 to 0.56 micrograms/rat), but it is 143 times more active than morphine. Naloxone, ICV or IP, antagonizes dermorp...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(83)90165-1
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Visfatin is a novel adipokine and proinflammatory cytokine which is implicated in breast cancer progression. The exact proliferative and anti-apoptotic mechanisms of visfatin are still under debate. In this study, the effect of extracellular visfatin on proliferation and apoptosis of breast cancer cells were...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2017.04.007
更新日期:2017-06-01 00:00:00
abstract::Selective GLP-1 secretagogues represent a novel potential therapy for type 2 diabetes mellitus. This study examined the GLP-1 secretory activity of the ethnomedicinal plant, Fagonia cretica, which is postulated to possess anti-diabetic activity. After extraction and fractionation extracts and purified compounds were t...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2016.01.015
更新日期:2016-04-01 00:00:00
abstract::The gut tissues and associated nervous system of the African migratory locust, Locusta migratoria, were found to contain FMRFamide-like immunoreactive (FLI) material throughout the five larval instars and 2 weeks into the adult stage in both males and females. FMRFamide-like immunoreactivity associated with the locust...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.08.013
更新日期:2003-10-01 00:00:00
abstract::It is well known that neuropeptide Y (NPY) and gamma-aminobutyric acid (GABA) exert antiepileptic effects in animal models. It has recently been shown that ghrelin neurons increase the activities of GABA and NPY in the brain. Therefore it can be said that ghrelin is an antiepileptic agent. In this study we aimed to in...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.04.003
更新日期:2007-06-01 00:00:00
abstract::Hymenochirin-1B (IKLSPETKDN(10)LKKVLKGAIK(20)GAIAVAKMV.NH2) is a cationic, amphipathic, α-helical, host-defense peptide, first isolated from skin secretions of the Congo clawed frog Hymenochirus boettgeri (Pipidae). Structure-activity relationships were investigated by synthesizing analogs in which the Pro(5), Glu(6) ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.10.015
更新日期:2013-12-01 00:00:00