Fine scale mapping in the sex locus region of the honey bee (Apis mellifera).

Abstract:

:Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. The construction of specific inbred crosses facilitates fine scale mapping in the sex locus region of the honey bee. The high recombination rate in the honey bee reduces the physical distance between markers compared with model organisms and facilitates a novel gene isolation strategy based on step-wise creation of new markers within small physical distances. We show that distances less than 25 kb can be efficiently mapped with a mapping population of only 1000 individuals. The procedure described here will accelerate the mapping, analysis and isolation of honey bee genes.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Hasselmann M,Fondrk MK,Page RE,Beye M

doi

10.1046/j.0962-1075.2001.00300.x

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

605-8

issue

6

eissn

0962-1075

issn

1365-2583

pii

300

journal_volume

10

pub_type

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