A Drosophila melanogaster hobo-white(+) vector mediates low frequency gene transfer in D. virilis with full interspecific white(+) complementation.

Abstract:

:Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white(+) cassette (H[w(+), hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one containing only the white(+) marked vector, and a sibling line containing the vector as well as an HFL1 helper integration. An approximate total transformation frequency of 1% is deduced. A high frequency of wing and eye morphology mutants were also observed, suggesting that hobo may have mobilized a related element in D. virilis. The data reaffirms a relatively low transformation vector activity for the hobo transposon in D. virilis; however, nearly full interspecific expression white(+) marker supports its possible function in other species as well.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Gomez SP,Handler AM

doi

10.1111/j.1365-2583.1997.tb00084.x

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

165-71

issue

2

eissn

0962-1075

issn

1365-2583

journal_volume

6

pub_type

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