Mobility assays confirm the broad host-range activity of the Minos transposable element and validate new transformation tools.

abstract：:Previous research has led to the idea that derived traits can arise through the evolution of novel roles for conserved genes. We explored whether neuropeptide Y (NPY)-like signalling, a conserved pathway that regulates food-related behaviour, is involved in a derived, nutritionally-related trait, the division of labou...

journal_title：Insect molecular biology

authors： Ament SA,Velarde RA,Kolodkin MH,Moyse D,Robinson GE

更新日期：2011-06-01 00:00:00

abstract：:We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

journal_title：Insect molecular biology

authors： Kiya T,Kunieda T,Kubo T

更新日期：2008-09-01 00:00:00

abstract：:Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...

journal_title：Insect molecular biology

authors： Duan SG,Li DZ,Wang MQ

更新日期：2019-02-01 00:00:00

abstract：:Hztransib, recently identified from Helicoverpa zea, represents the first intact and transcriptionally active Transib element. Its open reading frame was detected in Helicoverpa armigera, from which H. zea evolved, and in Helicoverpa assulta, the common ancestor of H. zea and H. armigera, but its remaining parts were ...

journal_title：Insect molecular biology

authors： Du E,Ni X,Zhao H,Li X

更新日期：2011-06-01 00:00:00

abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

journal_title：Insect molecular biology

authors： Biessmann H,Donath J,Walter MF

更新日期：1996-02-01 00:00:00

abstract：:PCR primers designed from the alpha-esterase gene cluster of Drosophila melanogaster have been used to isolate fragments from eight esterase genes in the Australian sheep blowfly, Lucilia cuprina. Phylogenetic analysis suggests that three are homologues of the alpha E7, alpha E8 and alpha E9 genes of the alpha-esteras...

journal_title：Insect molecular biology

authors： Newcomb RD,East PD,Russell RJ,Oakeshott JG

更新日期：1996-08-01 00:00:00

abstract：:Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...

journal_title：Insect molecular biology

authors： Foy BD,Myles KM,Pierro DJ,Sanchez-Vargas I,Uhlírová M,Jindra M,Beaty BJ,Olson KE

更新日期：2004-02-01 00:00:00

abstract：:Juvenile hormone esterase (JHE) activity in the haemolymph of the Colorado potato beetle is necessary to initiate pupation in larvae as well as diapause in adults. The enzyme appears in the haemolymph as a dimer consisting of two 57 kDa subunits. The sequence of an encoding cDNA, JHE.A, is distinct from lepidopteran J...

journal_title：Insect molecular biology

authors： Vermunt AM,Koopmanschap AB,Vlak JM,de Kort CA

更新日期：1998-11-01 00:00:00

abstract：:Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...

journal_title：Insect molecular biology

authors： Eguchi Y,Ihara M,Ochi E,Shibata Y,Matsuda K,Fushiki S,Sugama H,Hamasaki Y,Niwa H,Wada M,Ozoe F,Ozoe Y

更新日期：2006-12-01 00:00:00

abstract：:Speckled (Spc), an X-ray-induced lethal mutant of Bombyx mori, exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in females. Here, we report the morphological characterization and molecular mapping of the Spc mutant. Morphological investigations revealed that the epiderma...

journal_title：Insect molecular biology

authors： Tan D,Tong XL,Hu H,Wu SY,Li CL,Xiong G,Xiang ZH,Dai FY,Lu C

更新日期：2016-04-01 00:00:00

abstract：:Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows ...

journal_title：Insect molecular biology

authors： Corona M,Robinson GE

更新日期：2006-10-01 00:00:00

abstract：:Cyclobutane pyrimidine dimer (CPD) photolyases repair ultraviolet (UV)-induced DNA damage using blue light. To get insight in the origin of baculovirus CPD photolyase (phr) genes, homologues in the lepidopteran insects Chrysodeixis chalcites, Spodoptera exigua and Trichoplusia ni were identified and characterized. Lep...

journal_title：Insect molecular biology

authors： Biernat MA,Ros VI,Vlak JM,van Oers MM

更新日期：2011-08-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Droso...

journal_title：Insect molecular biology

authors： Tarnay JN,Szeri F,Iliás A,Annilo T,Sung C,Le Saux O,Váradi A,Dean M,Boyd CD,Robinow S

更新日期：2004-10-01 00:00:00

abstract：:Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

journal_title：Insect molecular biology

authors： Zandawala M,Hamoudi Z,Lange AB,Orchard I

更新日期：2015-04-01 00:00:00

abstract：:Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...

journal_title：Insect molecular biology

authors： Collins AM,Caperna TJ,Williams V,Garrett WM,Evans JD

更新日期：2006-10-01 00:00:00

abstract：:The piggyBac IFP2 transposable element, originally discovered in a Trichoplusia ni cell line, also exists as nearly identical elements in other noctuid lepidopterans, and in several species of the tephritid genus Bactrocera. To further define the distribution of piggyBacs in Bactrocera, and compare their relationship ...

journal_title：Insect molecular biology

authors： Handler AM,Zimowska GJ,Armstrong KF

更新日期：2008-08-01 00:00:00

abstract：:Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...

journal_title：Insect molecular biology

authors： Merlin C,Rosell G,Carot-Sans G,François MC,Bozzolan F,Pelletier J,Jacquin-Joly E,Guerrero A,Maïbèche-Coisne M

更新日期：2007-02-01 00:00:00

abstract：:Apis mellifera plays crucial roles in maintaining the balance of global ecosystems and stability of agricultural systems by helping pollination of flowering plants, including many crops. In recent years, this balance has been disrupted greatly by some pesticides, which results in great losses of honeybees worldwide. P...

journal_title：Insect molecular biology

authors： Hu YT,Tang CK,Wu CP,Wu PC,Yang EC,Tai CC,Wu YL

更新日期：2018-08-01 00:00:00

abstract：:We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...

journal_title：Insect molecular biology

authors： Sinclair BJ,Gibbs AG,Roberts SP

更新日期：2007-08-01 00:00:00

abstract：:The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of ...

journal_title：Insect molecular biology

authors： Gill M,Ellar D

更新日期：2002-12-01 00:00:00

abstract：:Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

journal_title：Insect molecular biology

authors： Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

更新日期：1992-01-01 00:00:00

abstract：:Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect m...

journal_title：Insect molecular biology

authors： Kambhampati S,Smith PT

更新日期：1995-11-01 00:00:00

abstract：:We aimed to understand the underlying mechanism that regulates successively expressed cuticular protein (CP) genes around pupation in Bombyx mori. Quantitative PCR was conducted to clarify the expression profile of CP genes and ecdysone-responsive transcription factor (ERTF) genes around pupation. Ecdysone pulse treat...

journal_title：Insect molecular biology

authors： Shahin R,Iwanaga M,Kawasaki H

更新日期：2016-04-01 00:00:00

abstract：:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...

journal_title：Insect molecular biology

authors： Pedra JH,Brandt A,Westerman R,Lobo N,Li HM,Romero-Severson J,Murdock LL,Pittendrigh BR

更新日期：2003-08-01 00:00:00

abstract：:Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...

journal_title：Insect molecular biology

authors： Petrova A,Smith CM

更新日期：2014-02-01 00:00:00

abstract：:RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major ...

journal_title：Insect molecular biology

authors： Balakrishna Pillai A,Nagarajan U,Mitra A,Krishnan U,Rajendran S,Hoti SL,Mishra RK

更新日期：2017-04-01 00:00:00

abstract：:A wide array of sensilla are distributed on insect antennae, and they play a variety of important roles. Rice planthoppers, destructive pests on rice, have a unique antenna sensilla structure called the 'sensory plaque organ'. The spineless (ss) gene encodes a bHLH-PAS transcription factor and plays a key role in ante...

journal_title：Insect molecular biology

authors： Li X,Liu FZ,Cai WL,Zhao J,Hua HX,Zou YL

更新日期：2019-04-01 00:00:00

abstract：:In Culex pipiens mosquitoes, A2 esterase alleles are co-amplified with B2 esterase alleles in response to selection with organophosphate insecticides. In this study the amplified A2 and B2 sequences were compared between twelve strains from four continents by restriction mapping. The restriction maps were almost ident...

journal_title：Insect molecular biology

authors： Callaghan A,Guillemaud T,Makate N,Raymond M

更新日期：1998-08-01 00:00:00

abstract：:Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...

journal_title：Insect molecular biology

authors： Norry FM,Sambucetti P,Scannapieco AC,Gomez FH,Loeschcke V

更新日期：2007-08-01 00:00:00

abstract：:Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...

journal_title：Insect molecular biology

authors： Yang F,Zhu B,Liu J,Liu Y,Jiang C,Sheng Q,Qiu J,Nie Z

更新日期：2020-02-01 00:00:00