Transcriptome analysis of the cowpea weevil bruchid: identification of putative proteinases and alpha-amylases associated with food breakdown.

Abstract:

:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. Twenty-three inferred genes potentially involved in digestive processes in cowpea weevil were identified, including proteinases and amylases. The predicted catalytic sites were identified in the inferred cysteine and aspartic acid proteinases, and in alpha-amylases. Transcriptome analysis of the cowpea bruchid will potentially permit gene discovery in other beetles, an insect order of major economic and ecological importance that is poorly represented in genomic databases.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Pedra JH,Brandt A,Westerman R,Lobo N,Li HM,Romero-Severson J,Murdock LL,Pittendrigh BR

doi

10.1046/j.1365-2583.2003.00425.x

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

405-12

issue

4

eissn

0962-1075

issn

1365-2583

pii

425

journal_volume

12

pub_type

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