Abstract:
:The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. In the present study, we cloned and characterized the cDNA of Laodelphax striatellus Dscam (LsDscam) and investigated the function of LsDscam in rice stripe virus (RSV) infection and the influence on the endosymbiont Wolbachia. LsDscam displayed a typical Dscam domain architecture, including 10 immunoglobulin (Ig) domains, six fibronectin type III domains, one transmembrane domain and a cytoplasmic tail. Alternative splicing occurred at the N-termini of the Ig2 and Ig3 domains, the complete Ig7 domain, the transmembrane domain and the C-terminus, comprising 10, 51, 35, two and two variable exons, respectively. Potentially LsDscam could encode at least 71 400 unique isoforms and 17 850 types of extracellular regions. LsDscam was expressed in various L. striatellus tissues. Knockdown of LsDscam mRNA via RNA interference decreased the titres of both RSV and Wolbachia, but did not change the numbers of the extracellular symbiotic bacterium Acinetobacter rhizosphaerae. Specific Dscam isoforms may play roles in enhancing the infection of vector-borne viruses or endosymbionts.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Zhang F,Li Q,Chen X,Huo Y,Guo H,Song Z,Cui F,Zhang L,Fang Rdoi
10.1111/imb.12226subject
Has Abstractpub_date
2016-08-01 00:00:00pages
413-21issue
4eissn
0962-1075issn
1365-2583journal_volume
25pub_type
杂志文章abstract::A novel receptor guanylyl cyclase (GC) has been identified from the oriental fruit fly Bactrocera dorsalis (Hendel) and has been designated BdmGC-1. Protein domain analysis revealed that BdmGC-1 possesses a characteristic domain organization similar to all known receptor GCs but with a unique carboxyl-terminal extensi...
journal_title:Insect molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Insect molecular biology
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pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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