N- and C-terminal degradation of ecdysteroid receptor isoforms, when transiently expressed in mammalian CHO cells, is regulated by the proteasome and cysteine and threonine proteases.

Abstract:

:Transcriptional activity of nuclear receptors is the result of transactivation capability and the concentration of the receptor protein. The concentration of ecdysteroid receptor (EcR) isoforms, constitutively expressed in mammalian CHO cells, is dependent on a number of factors. As shown previously, ligand binding stabilizes receptor protein concentration. In this paper, we investigate the degradation of EcR isoforms and provide evidence that N-terminal degradation is modulated by isoform-specific ubiquitination sites present in the A/B domains of EcR-A and -B1. This was demonstrated by the increase in EcR concentration by treatment with carbobenzoxy-L-leucyl-L-leucyl-L-leucinal (MG132), an inhibitor of ubiquitin-mediated proteasomal degradation and by deletion of ubiquitination sites. In addition, EcR is degraded by the peptidyl-dipeptidase cathepsin B (CatB) and the endopeptidase cathepsin S (CatS) at the C-terminus in an isoform-specific manner, despite identical C-termini. Ubiquitin-proteasome-mediated degradation and the proteolytic action are modulated by heterodimerization with Ultraspiracle (USP). The complex regulation of receptor protein concentration offers an additional opportunity to regulate transcriptional activity in an isoform- and target cell-specific way and allows the temporal limitation of hormone action.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Schauer S,Burster T,Spindler-Barth M

doi

10.1111/j.1365-2583.2012.01144.x

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

383-94

issue

3

eissn

0962-1075

issn

1365-2583

journal_volume

21

pub_type

杂志文章
  • Serine proteases identified from a Costelytra zealandica (White) (Coleoptera: Scarabaeidae) midgut EST library and their expression through insect development.

    abstract::Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00798.x

    authors: Marshall SD,Gatehouse LN,Becher SA,Christeller JT,Gatehouse HS,Hurst MR,Boucias DG,Jackson TA

    更新日期:2008-06-01 00:00:00

  • Insect genomes: progress and challenges.

    abstract::In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...

    journal_title:Insect molecular biology

    pub_type: 杂志文章,评审

    doi:10.1111/imb.12599

    authors: Li F,Zhao X,Li M,He K,Huang C,Zhou Y,Li Z,Walters JR

    更新日期:2019-12-01 00:00:00

  • Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap-3) by a new class-II piggyBac-related insect transposon.

    abstract::A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00917.x

    authors: Carpes MP,Nunes JF,Sampaio TL,Castro ME,Zanotto PM,Ribeiro BM

    更新日期:2009-11-01 00:00:00

  • Hidden from the host: Spiroplasma bacteria infecting Drosophila do not cause an immune response, but are suppressed by ectopic immune activation.

    abstract::Insects and other arthropods have an effective innate immune system that can clear infections with bacteria and other microorganisms. Despite this ability, one group of bacteria, the spiroplasmas, survive unharmed within the haemolymph of a wide range of arthropod hosts. We investigated the interaction between one mem...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00380.x

    authors: Hurst GD,Anbutsu H,Kutsukake M,Fukatsu T

    更新日期:2003-02-01 00:00:00

  • Wing development genes of the pea aphid and differential gene expression between winged and unwinged morphs.

    abstract::Little is known about when, how or even if the wing development gene network elucidated in Drosophila is deployed in direct-developing insects. Here we identify the wing development genes (as determined in Drosophila) of the pea aphid (Acyrthosiphon pisum), which produces winged or unwinged adults in response to envir...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00935.x

    authors: Brisson JA,Ishikawa A,Miura T

    更新日期:2010-03-01 00:00:00

  • Preferential codon usage and two types of repetitive motifs in the fibroin gene of the Chinese oak silkworm, Antheraea pernyi.

    abstract::In this paper we describe the peculiar structures and preferential codon usage found in wild silkworm fibroin genes. We determined a 1350 bp nucleotide sequence from the Chinese oak silkworm, Antheraea pernyi. The deduced amino acid sequence was partitioned into thirteen polyalanine-containing repetitive motifs, which...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1997.00161.x

    authors: Yukuhiro K,Kanda T,Tamura T

    更新日期:1997-02-01 00:00:00

  • Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.

    abstract::Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00643.x

    authors: Shi L,Li B,Paskewitz SM

    更新日期:2006-06-01 00:00:00

  • Baculovirus cyclobutane pyrimidine dimer photolyases show a close relationship with lepidopteran host homologues.

    abstract::Cyclobutane pyrimidine dimer (CPD) photolyases repair ultraviolet (UV)-induced DNA damage using blue light. To get insight in the origin of baculovirus CPD photolyase (phr) genes, homologues in the lepidopteran insects Chrysodeixis chalcites, Spodoptera exigua and Trichoplusia ni were identified and characterized. Lep...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2011.01076.x

    authors: Biernat MA,Ros VI,Vlak JM,van Oers MM

    更新日期:2011-08-01 00:00:00

  • A venom metalloproteinase from the parasitic wasp Eulophus pennicornis is toxic towards its host, tomato moth (Lacanobia oleracae).

    abstract::Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00864.x

    authors: Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

    更新日期:2009-04-01 00:00:00

  • Molecular characterization of Ephestia kuehniella (Lepidoptera: Pyralidae) transferrin and its response to parasitoid Venturia canescens (Hymenoptera: Ichneumonidae Gravenhorst).

    abstract::In the present study, we characterized a full-length cDNA encoding a putative iron-binding protein transferrin from the lepidopteran Mediterranean flour moth (EkTrf, 2397 bp). The putative EkTrf is 683 amino acids with a molecular mass of approximately 76 kDa. The deduced amino acid sequence showed significant homolog...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2011.01129.x

    authors: Guz N,Kilincer N,Aksoy S

    更新日期:2012-04-01 00:00:00

  • Analysis of intraspecies polymorphism in the ribosomal DNA cluster of the cockroach Blattella germanica.

    abstract::HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2000.00175.x

    authors: Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal C

    更新日期:2000-04-01 00:00:00

  • Amelogenin domain-containing NlChP38 is necessary for normal ovulation in the brown planthopper.

    abstract::The brown planthopper (BPH), Nilaparvata lugens, is a major threat to rice production. The eggshell plays an important role in insect reproduction. The constituents and formation process of BPH eggshells remains largely unknown. Here, we report a novel eggshell-associated protein, NlChP38, containing an amelogenin dom...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12576

    authors: Lou YH,Lu JB,Li DT,Ye YX,Luo XM,Zhang CX

    更新日期:2019-10-01 00:00:00

  • Antennal esterase cDNAs from two pest moths, Spodoptera littoralis and Sesamia nonagrioides, potentially involved in odourant degradation.

    abstract::Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00702.x

    authors: Merlin C,Rosell G,Carot-Sans G,François MC,Bozzolan F,Pelletier J,Jacquin-Joly E,Guerrero A,Maïbèche-Coisne M

    更新日期:2007-02-01 00:00:00

  • Microarray analysis of genes showing variable expression following a blood meal in Anopheles gambiae.

    abstract::A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00567.x

    authors: Marinotti O,Nguyen QK,Calvo E,James AA,Ribeiro JM

    更新日期:2005-08-01 00:00:00

  • X-linked QTL for knockdown resistance to high temperature in Drosophila melanogaster.

    abstract::Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2007.00747.x

    authors: Norry FM,Sambucetti P,Scannapieco AC,Gomez FH,Loeschcke V

    更新日期:2007-08-01 00:00:00

  • Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L.

    abstract::To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00911.x

    authors: Tadano H,Yamazaki Y,Takeuchi H,Kubo T

    更新日期:2009-11-01 00:00:00

  • Insect transformation with piggyBac: getting the number of injections just right.

    abstract::The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilized for insect germline transformation. The described ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12220

    authors: Gregory M,Alphey L,Morrison NI,Shimeld SM

    更新日期:2016-06-01 00:00:00

  • An expansion of the genome size dataset for the insect order Hymenoptera, with a first test of parasitism and eusociality as possible constraints.

    abstract::Although the Hymenoptera represent a remarkably diverse and socioeconomically important group that is of considerable interest in genome biology, they remain understudied in terms of genome size. This study reports new genome size estimates for 89 species of ants, bees and wasps, representing 17 families and four supe...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.00992.x

    authors: Ardila-Garcia AM,Umphrey GJ,Gregory TR

    更新日期:2010-06-01 00:00:00

  • The Aedes aegypti larval transcriptome: a comparative perspective with emphasis on trypsins and the domain structure of peritrophins.

    abstract::The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adu...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00845.x

    authors: Venancio TM,Cristofoletti PT,Ferreira C,Verjovski-Almeida S,Terra WR

    更新日期:2009-02-01 00:00:00

  • Efficacy of RNA interference knockdown using aerosolized short interfering RNAs bound to nanoparticles in three diverse aphid species.

    abstract::RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12301

    authors: Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

    更新日期:2017-06-01 00:00:00

  • A member of the hsp70 gene family from the Mediterranean fruit fly, Ceratitis capitata.

    abstract::A gene from the Mediterranean fruit fly (Medfly) Ceratitis capitata, belonging to the hsp70 family of genes, has been cloned and characterized. This gene appears to be an example of a heat shock cognate (hsc) gene based on a variety of structural and functional criteria. The heat shock cognate genes serve critical rol...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1998.71051.x

    authors: Thanaphum S,Haymer DS

    更新日期:1998-02-01 00:00:00

  • Fine scale mapping in the sex locus region of the honey bee (Apis mellifera).

    abstract::Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. T...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.0962-1075.2001.00300.x

    authors: Hasselmann M,Fondrk MK,Page RE,Beye M

    更新日期:2001-12-01 00:00:00

  • How do hosts react to endosymbionts? A new insight into the molecular mechanisms underlying the Wolbachia-host association.

    abstract::Wolbachia is an intracellular bacterium that has aroused intense interest because of its ability to alter the biology of its host in diverse ways. In the two-spotted spider mite, Tetranychus urticae, Wolbachia can induce complex cytoplasmic incompatibility (CI) phenotypes and fitness changes, although little is known ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12128

    authors: Zhang YK,Ding XL,Rong X,Hong XY

    更新日期:2015-02-01 00:00:00

  • Cytochrome P450s CYP6D3 and CYP6D1 are part of a P450 gene cluster on autosome 1 in the house fly.

    abstract::The P450 monooxygenases of insects are important in the metabolism of numerous endogenous and exogenous compounds. However, identity of the P450 isoform(s) involved in these reactions is rarely known. A critical first step in the identification of important P450s is the cloning and sequencing of their genes. Toward th...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2001.00256.x

    authors: Kasai S,Scott JG

    更新日期:2001-06-01 00:00:00

  • Preservation of a transgenic strain of the sawfly, Athalia rosae (Hymenoptera) by artificial fertilization using cryopreserved sperm.

    abstract::Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00526.x

    authors: Hatakeyama M,Sumitani M

    更新日期:2005-01-01 00:00:00

  • The expression of the HSP90 gene in response to winter and summer diapauses and thermal-stress in the onion maggot, Delia antiqua.

    abstract::The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00602.x

    authors: Chen B,Kayukawa T,Monteiro A,Ishikawa Y

    更新日期:2005-12-01 00:00:00

  • Two fatty acid synthase genes from the integument contribute to cuticular hydrocarbon biosynthesis and cuticle permeability in Locusta migratoria.

    abstract::Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, L...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12665

    authors: Yang Y,Zhao X,Niu N,Zhao Y,Liu W,Moussian B,Zhang J

    更新日期:2020-12-01 00:00:00

  • Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori.

    abstract::E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12244

    authors: Tanaka H,Sagisaka A,Suzuki N,Yamakawa M

    更新日期:2016-10-01 00:00:00

  • Pyrosequencing and characterization of immune response genes from the American dog tick, Dermacentor variabilis (L.).

    abstract::Ticks continue to be a threat to animal and human health, and new and novel control strategies are needed for ticks and tick-borne pathogens. The characterization of the tick-pathogen interface and the tick immune response to microbial infections is fundamental toward the formulation of new control strategies for tick...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01037.x

    authors: Jaworski DC,Zou Z,Bowen CJ,Wasala NB,Madden R,Wang Y,Kocan KM,Jiang H,Dillwith JW

    更新日期:2010-10-01 00:00:00

  • Transfection of cultured cells of the cotton boll weevil, Anthonomus grandis, with a heat-shock-promoter-chloramphenicol-acetyltransferase construct.

    abstract::Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1993.tb00108.x

    authors: Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

    更新日期:1992-01-01 00:00:00