Abstract:
:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal domain of unknown function. EpMP3 expressed as a recombinant protein in Pichia pastoris had gelatinase activity, which was inhibited by EDTA. Injection of recombinant EpMP3 into fifth instar Lacanobia oleracea (host) larvae resulted in partial insect mortality associated with the moult to sixth instar, with surviving insects showing retarded development and growth. EpMP3 is expressed specifically in venom glands. These results suggest that EpMP3 is a functional component of Eulophus venom, which is able to manipulate host development.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JAdoi
10.1111/j.1365-2583.2009.00864.xsubject
Has Abstractpub_date
2009-04-01 00:00:00pages
195-202issue
2eissn
0962-1075issn
1365-2583pii
IMB864journal_volume
18pub_type
杂志文章abstract::PCR primers designed from the alpha-esterase gene cluster of Drosophila melanogaster have been used to isolate fragments from eight esterase genes in the Australian sheep blowfly, Lucilia cuprina. Phylogenetic analysis suggests that three are homologues of the alpha E7, alpha E8 and alpha E9 genes of the alpha-esteras...
journal_title:Insect molecular biology
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doi:10.1111/j.1365-2583.1996.tb00056.x
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abstract::The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...
journal_title:Insect molecular biology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1999.00139.x
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abstract::We recently showed that the Masculinizer gene (Masc) plays a primary role in sex determination in the lepidopteran model insect Bombyx mori. However, it remains unknown whether this Masc protein-dependent sex determination system is conserved amongst lepidopteran insects or within the family Bombycidae. Here we cloned...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12181
更新日期:2015-10-01 00:00:00
abstract::The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12117
更新日期:2014-12-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
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更新日期:2018-08-01 00:00:00
abstract::Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12613
更新日期:2020-02-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2008.00821.x
更新日期:2008-09-01 00:00:00
abstract::Phosphofructokinase (PFK) is a rate-limiting enzyme in glycolysis, but its linkage with locomotion in termites is not well understood, despite the demonstrated involvement of this gene in the locomotion of different animals. Here, we investigated the effect of the pfk gene on locomotion in the subterranean termite Ret...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12672
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abstract::We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00653.x
更新日期:2006-08-01 00:00:00
abstract::Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases (CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12678
更新日期:2021-02-01 00:00:00
abstract::Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2012.01145.x
更新日期:2012-08-01 00:00:00
abstract::Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.0962-1075.2001.00284.x
更新日期:2001-10-01 00:00:00
abstract::Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00915.x
更新日期:2010-02-01 00:00:00
abstract::Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12602
更新日期:2019-12-01 00:00:00
abstract::In the ant Camponotus festinatus, two different hexamerins accumulate stage-specifically during the late larval period and at various times in adults. These hexamerins serve as storage proteins and play important roles in brood nourishment and colony founding. We report an analysis of the cDNA sequence of C. festinatu...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00204.x
更新日期:2000-08-01 00:00:00
abstract::The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Med...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00737.x
更新日期:2007-08-01 00:00:00
abstract::Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00674.x
更新日期:2006-10-01 00:00:00
abstract::The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12130
更新日期:2014-12-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
abstract::The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1994.tb00142.x
更新日期:1993-01-01 00:00:00
abstract::The P450 monooxygenases of insects are important in the metabolism of numerous endogenous and exogenous compounds. However, identity of the P450 isoform(s) involved in these reactions is rarely known. A critical first step in the identification of important P450s is the cloning and sequencing of their genes. Toward th...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00256.x
更新日期:2001-06-01 00:00:00
abstract::Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12058
更新日期:2014-02-01 00:00:00
abstract::A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00249.x
更新日期:2001-04-01 00:00:00
abstract::Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.0962-1075.2004.00520.x
更新日期:2004-12-01 00:00:00
abstract::In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...
journal_title:Insect molecular biology
pub_type: 杂志文章,评审
doi:10.1111/imb.12599
更新日期:2019-12-01 00:00:00
abstract::The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily of pheromone/odourant binding proteins. Although D7 proteins are among the most abundant salivary proteins in adult female mosquitoes and sand flies, their role in blood feeding remains elusive. In the present w...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2002.00319.x
更新日期:2002-04-01 00:00:00
abstract::We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference be...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00628.x
更新日期:2006-04-01 00:00:00
abstract::Insulin-like peptides (ILPs) including insulin, insulin-like growth factor (IGF) and relaxin are evolutionarily conserved hormones in metazoans, and they are involved in diverse physiological processes. The migratory brown planthopper (BPH), Nilaparvata lugens, encodes four ILP genes (Nlilp1, Nlilp2, Nlilp3 and Nlilp4...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12636
更新日期:2020-06-01 00:00:00