A venom metalloproteinase from the parasitic wasp Eulophus pennicornis is toxic towards its host, tomato moth (Lacanobia oleracae).

Abstract:

:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal domain of unknown function. EpMP3 expressed as a recombinant protein in Pichia pastoris had gelatinase activity, which was inhibited by EDTA. Injection of recombinant EpMP3 into fifth instar Lacanobia oleracea (host) larvae resulted in partial insect mortality associated with the moult to sixth instar, with surviving insects showing retarded development and growth. EpMP3 is expressed specifically in venom glands. These results suggest that EpMP3 is a functional component of Eulophus venom, which is able to manipulate host development.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

doi

10.1111/j.1365-2583.2009.00864.x

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

195-202

issue

2

eissn

0962-1075

issn

1365-2583

pii

IMB864

journal_volume

18

pub_type

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