Hidden from the host: Spiroplasma bacteria infecting Drosophila do not cause an immune response, but are suppressed by ectopic immune activation.

abstract：:Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...

journal_title：Insect molecular biology

authors： Foy BD,Myles KM,Pierro DJ,Sanchez-Vargas I,Uhlírová M,Jindra M,Beaty BJ,Olson KE

更新日期：2004-02-01 00:00:00

abstract：:Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

journal_title：Insect molecular biology

authors： Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

更新日期：1992-01-01 00:00:00

abstract：:Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...

journal_title：Insect molecular biology

authors： Lin Y,Liu H,Yang C,Gu J,Shen G,Zhang H,Chen E,Han C,Zhang Y,Xu Y,Wu J,Xia Q

更新日期：2017-10-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...

journal_title：Insect molecular biology

authors： Nagoshi RN,Meagher R

更新日期：2003-10-01 00:00:00

abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

journal_title：Insect molecular biology

authors： Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

更新日期：1994-05-01 00:00:00

abstract：:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...

journal_title：Insect molecular biology

authors： Pedra JH,Brandt A,Westerman R,Lobo N,Li HM,Romero-Severson J,Murdock LL,Pittendrigh BR

更新日期：2003-08-01 00:00:00

abstract：:The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...

journal_title：Insect molecular biology

authors： Fenn JD,Cameron SL,Whiting MF

更新日期：2007-04-01 00:00:00

abstract：:A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...

journal_title：Insect molecular biology

authors： Zhao JT,Bennett CL,Stewart GJ,Frommer M,Raphael KA

更新日期：2003-06-01 00:00:00

abstract：:Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...

journal_title：Insect molecular biology

authors： Pannebakker BA,Niehuis O,Hedley A,Gadau J,Shuker DM

更新日期：2010-02-01 00:00:00

abstract：:E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...

journal_title：Insect molecular biology

authors： Tanaka H,Sagisaka A,Suzuki N,Yamakawa M

更新日期：2016-10-01 00:00:00

abstract：:Baculovirus-host interactions are important models for studying the biological control of lepidopteran pests. Research on baculovirus-host interactions has focussed on baculovirus manipulation of cellular signalling pathways, including the extracellular signal-regulated kinase (ERK) and phosphatidylinositol-3-kinases/...

journal_title：Insect molecular biology

authors： Jin S,Cheng T,Guo Y,Lin P,Zhao P,Liu C,Kusakabe T,Xia Q

更新日期：2018-08-01 00:00:00

abstract：:To begin to explore the molecular dynamics of rickettsial tick interaction, subtractive hybridization was used to screen mRNAs in Rickettsia montanensis-infected and uninfected Dermacentor variabilis. We isolated 30 cDNA fragments, 22 of which were up-regulated and eight were down-regulated in response to rickettsial ...

journal_title：Insect molecular biology

authors： Mulenga A,Macaluso KR,Simser JA,Azad AF

更新日期：2003-04-01 00:00:00

abstract：:The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilized for insect germline transformation. The described ...

journal_title：Insect molecular biology

authors： Gregory M,Alphey L,Morrison NI,Shimeld SM

更新日期：2016-06-01 00:00:00

abstract：:Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDN...

journal_title：Insect molecular biology

authors： Gaines PJ,Sampson CM,Rushlow KE,Stiegler GL

更新日期：1999-02-01 00:00:00

abstract：:PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...

journal_title：Insect molecular biology

authors： Thompson GJ,Crozier YC,Crozier RH

更新日期：2003-02-01 00:00:00

abstract：:Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

journal_title：Insect molecular biology

authors： Zandawala M,Hamoudi Z,Lange AB,Orchard I

更新日期：2015-04-01 00:00:00

abstract：:We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...

journal_title：Insect molecular biology

authors： Sinclair BJ,Gibbs AG,Roberts SP

更新日期：2007-08-01 00:00:00

abstract：:Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...

journal_title：Insect molecular biology

authors： Petrova A,Smith CM

更新日期：2014-02-01 00:00:00

abstract：:Previous research has led to the idea that derived traits can arise through the evolution of novel roles for conserved genes. We explored whether neuropeptide Y (NPY)-like signalling, a conserved pathway that regulates food-related behaviour, is involved in a derived, nutritionally-related trait, the division of labou...

journal_title：Insect molecular biology

authors： Ament SA,Velarde RA,Kolodkin MH,Moyse D,Robinson GE

更新日期：2011-06-01 00:00:00

abstract：:Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...

journal_title：Insect molecular biology

authors： Merlin C,Rosell G,Carot-Sans G,François MC,Bozzolan F,Pelletier J,Jacquin-Joly E,Guerrero A,Maïbèche-Coisne M

更新日期：2007-02-01 00:00:00

abstract：:Glutathione transferases (GSTs) are a diverse family of enzymes found ubiquitously in aerobic organisms. They play a central role in the detoxification of both endogenous and xenobiotic compounds and are also involved in intracellular transport, biosynthesis of hormones and protection against oxidative stress. Interes...

journal_title：Insect molecular biology

authors： Enayati AA,Ranson H,Hemingway J

更新日期：2005-01-01 00:00:00

abstract：:In fat body of the mosquito, Aedes aegypti, a cycle of ribosome accumulation and degradation accompanies synthesis of the yolk protein precursor, vitellogenin. Here we compare the transcription and translation of ribosomal proteins rpS6, rpL8 and rpL34, relative to rRNA and vitellogenin genes in Aedes aegypti fat body...

journal_title：Insect molecular biology

authors： Niu LL,Fallon AM

更新日期：2000-12-01 00:00:00

abstract：:A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...

journal_title：Insect molecular biology

authors： Marinotti O,Nguyen QK,Calvo E,James AA,Ribeiro JM

更新日期：2005-08-01 00:00:00

abstract：:Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...

journal_title：Insect molecular biology

authors： Yang F,Zhu B,Liu J,Liu Y,Jiang C,Sheng Q,Qiu J,Nie Z

更新日期：2020-02-01 00:00:00

abstract：:Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...

journal_title：Insect molecular biology

authors： Gu J,Huang LX,Shen Y,Huang LH,Feng QL

更新日期：2012-10-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp24D) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 271 amino acid protein that contains the conserved serine, histidine and aspartic acid residues found in serine proteases, and has the highest identity to ...

journal_title：Insect molecular biology

authors： Han YS,Salazar CE,Reese-Stardy SR,Cornel A,Gorman MJ,Collins FH,Paskewitz SM

更新日期：1997-11-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prop...

journal_title：Insect molecular biology

authors： Paskewitz SM,Reese-Stardy S,Gorman MJ

更新日期：1999-08-01 00:00:00

abstract：:Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...

journal_title：Insect molecular biology

authors： Luo C,Shen B,Manley JL,Zheng L

更新日期：2001-10-01 00:00:00

abstract：:We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other specie...

journal_title：Insect molecular biology

authors： Scott MJ,Kriticou D,Robinson AS

更新日期：1993-01-01 00:00:00