Cloning of a family of serine protease genes from the cat flea Ctenocephalides felis.

Abstract:

:Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDNAs encoding mature serine proteases were subsequently cloned and sequenced. All of the mature proteases contained the histidine, aspartic acid and serine amino acids of the catalytic triad characteristic of serine proteases. The mature C. felis serine proteases had amino acid sequences that were at most 29-53% identical to those known insect and arachnid serine proteases. Two of the C. felis gene sequences had similarity with the Drosophila melanogaster developmental genes snake and stubble. mRNA expression of selected serine protease genes was examined in different life stages, tissues, genders, and in response to bloodfeeding.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Gaines PJ,Sampson CM,Rushlow KE,Stiegler GL

doi

10.1046/j.1365-2583.1999.810011.x

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

11-22

issue

1

eissn

0962-1075

issn

1365-2583

journal_volume

8

pub_type

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