Abstract：
:Culex pipiens quinquefasciatus Say and Culex pipiens pipiens Linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. The two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic traits. Within the North American zone of sympatry, populations of Cx. p. quinquefasciatus and Cx. p. pipiens undergo extensive introgression and hybrid forms have been reported in nature. Both Cx. p. quinquefasciatus and Cx. p. pipiens are infected with the endosymbiotic bacteria Wolbachia pipientis. Here, we report the presence of a transposable element belonging to the IS256 family (IS256wPip) associated with Wolbachia in both Cx. p. quinquefasciatus and Cx. p. pipiens populations. Using reverse transcriptase PCR and sequence analysis, we show that IS256wPip has disrupted the wspB locus, a paralogue of the Wolbachia outer membrane protein (wspA) gene. The inactivation of the wspB appears to be specific to Cx. p. quinquefasciatus and to hybrids of the two forms, and was not observed in the surveyed Cx. p. pipiens mosquitoes. Our results support the hypothesis of a different origin of North American Cx. p. quinquefasciatus and Cx. p. pipiens populations. The flux of mobile genetic elements in the Wolbachia wPip genome could explain the high level of crossing types observed among different Culex populations. The insertion of IS256wPip into wspB may comprise a genetic candidate for discriminating Wolbachia symbionts in Culex.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Sanogo YO,Dobson SL,Bordenstein SR,Novak RJdoi
10.1111/j.1365-2583.2006.00707.xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
143-54issue
2eissn
0962-1075issn
1365-2583pii
IMB707journal_volume
16pub_type
杂志文章abstract：:Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...
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journal_title：Insect molecular biology
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更新日期：2001-08-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：2020-02-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12390
更新日期：2018-08-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2011.01085.x
更新日期：2011-10-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2001.00256.x
更新日期：2001-06-01 00:00:00
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journal_title：Insect molecular biology
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doi：10.1111/j.1365-2583.2009.00911.x
更新日期：2009-11-01 00:00:00
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journal_title：Insect molecular biology
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